Sensitive Aptamer SERS and RRS Assays for Trace Oxytetracycline Based on the Catalytic Amplification of CuNCs

A new method for the determination of oxytetracycline (OTC) has been established by coupling the catalytic amplification reaction of copper nanoclusters (CuNCs) with the aptamer reaction. CuNCs prepared by a wet chemical method have the catalytic activity for the formation of gold nanoparticles (AuNPs) resulting from a HAuCl4-ethanol (En) reaction. The experimental results showed that OTC aptamer (Apt) can be adsorbed on the surface of CuNCs in a non-specific way, thus inhibiting its catalytic activity. When OTC was added to the solution, the OTC-Apt complex was generated by a specific reaction, which made the CuNCs desorb and restore their catalytic activity. With the increase of OTC, the recovery of the catalytic activity of CuNCs is strengthened, the reaction speed is accelerated, and the number of AuNPs is increased. The generated AuNPs exhibited surface enhanced Raman scattering (SERS) signals at 1615 cm−1 in the presence of Vitoria blue 4R (VB4R) molecular probes, and a resonance Rayleigh scattering (RRS) peak at 586 nm. There is a good linear relationship between the intensities of SERS, or RRS, and OTC concentration at the range of 37.5–300 ng/L or 37.5–225 ng/L, respectively. A new SERS and RRS assay for the determination of trace OTC based on the regulation of CuNCs catalysis was established.

Download Full-text

A Highly Sensitive SERS and RRS Coupled Di-Mode Method for CO Detection Using Nanogolds as Catalysts and Bifunctional Probes

Nanomaterials ◽

10.3390/nano10030450 ◽

2020 ◽

Vol 10 (3) ◽

pp. 450 ◽

Cited By ~ 1

Author(s):

Dongmei Yao ◽

Guiqing Wen ◽

Lingbo Gong ◽

Chongning Li ◽

Aihui Liang ◽

...

Keyword(s):

Rayleigh Scattering ◽

Molecular Probes ◽

Resonance Rayleigh Scattering ◽

Highly Sensitive ◽

Surface Enhanced ◽

Mode Method ◽

Surface Enhanced Raman ◽

Enhanced Raman Scattering ◽

Co Detection

Carbon monoxide (CO) is a commonly poisonous gas. It is important to detect CO in daily life. Herein, a new and sensitive surface enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) coupled di-mode method was developed for CO, based on gold nano-enzyme catalysis and gold nanoprobes. CO can react with HAuCl4 to generate gold nanoparticles (AuNPs) in pH 5.2 HAc-NaAc buffer. The generated AuNPs exhibited SERS activity at 1620 cm−1 in the presence of Vitoria blue B (VBB) molecular probes, and an RRS peak at 290 nm. Based on the AuNP bifunctional probes, the increased SERS and RRS intensities respond linearly with the concentration of CO in the range of 100–1500 ng/mL and 30–5230 ng/mL, respectively. To improve the sensitivity, the produced AuNPs were used as nano-enzyme catalysts for the new indicator reaction of HAuCl4-ethanol (En) to amplify the signal. The sensitive SERS method was coupled with the accurate RRS method to develop a sensitive and accurate SERS/RRS di-mode method for determination of 3.0–413 ng/mL CO, based on the AuNP-HAuCl4-En nanocatalytic reaction and its product of AuNPs as SERS and RRS bifunctional probes.

Download Full-text

Development of thin-film tunable band-pass filters based hyper-spectral imaging system applied for both surface enhanced Raman scattering and plasmon resonance Rayleigh scattering

Review of Scientific Instruments ◽

10.1063/1.4763572 ◽

2012 ◽

Vol 83 (10) ◽

pp. 103707 ◽

Cited By ~ 8

Author(s):

Mitsuhiro Iga ◽

Nobuyuki Kakuryu ◽

Takeo Tanaami ◽

Jiro Sajiki ◽

Katsumi Isozaki ◽

...

Keyword(s):

Thin Film ◽

Rayleigh Scattering ◽

Imaging System ◽

Resonance Rayleigh Scattering ◽

Hyper Spectral ◽

Surface Enhanced ◽

Surface Enhanced Raman ◽

Hyper Spectral Imaging ◽

Enhanced Raman Scattering ◽

Band Pass

Download Full-text

Doped N/Ag Carbon Dot Catalytic Amplification SERS Strategy for Acetamiprid Coupled Aptamer with 3,3′-Dimethylbiphenyl-4,4′-diamine Oxidizing Reaction

Nanomaterials ◽

10.3390/nano9030480 ◽

2019 ◽

Vol 9 (3) ◽

pp. 480 ◽

Cited By ~ 8

Author(s):

Xiaozhen Feng ◽

Chongning Li ◽

Aihui Liang ◽

Yanghe Luo ◽

Zhiliang Jiang

Keyword(s):

Catalytic Activity ◽

Analysis Method ◽

Carbon Dot ◽

Surface Enhanced ◽

Surface Enhanced Raman ◽

Enhanced Raman Scattering ◽

H2o2 Oxidation ◽

Quantitative Sers ◽

Co Doped

The as-prepared co-doped N/Ag carbon dot (CDNAg) has strong catalysis of H2O2 oxidation of 3,3′-dimethylbiphenyl-4,4′-diamine (DBD). It forms an oxidation product (DBDox) with surface-enhanced Raman scattering (SERS) activity at 1605 cm−1 in the silver nanosol substrate, and a CDNAg catalytic amplification with SERS analytical platform can be structured based on aptamer (Apt) with the DBD oxidizing reaction. For example, the aptamer (Apt) of acetamiprid (ACT) can be adsorbed on the surface of CDNAg, resulting in inhibited catalytic activity, the reduced generation of DBDox, and a weakened SERS intensity. When the target molecule ACT was added, it formed a stable Apt-ACT complex and free CDNAg that restored catalytic activity and linearly enhanced the SERS signal. Based on this, we proposed a new quantitative SERS analysis method for the determination of 0.01–1.5 μg ACT with a detection limit of 0.006 μg/L.

Download Full-text

Facile synthesis of a highly SERS active nanosilver sol using microwaves and its application in the detection of E. coli using Victoria blue B as a molecular probe

Analytical Methods ◽

10.1039/c6ay00728g ◽

2016 ◽

Vol 8 (24) ◽

pp. 4881-4887 ◽

Cited By ~ 6

Author(s):

Yaohui Wang ◽

Xinghui Zhang ◽

Guiqing Wen ◽

Aihui Liang ◽

Zhiliang Jiang

Keyword(s):

Silver Nanoparticles ◽

Raman Scattering ◽

Rayleigh Scattering ◽

Molecular Probe ◽

Resonance Rayleigh Scattering ◽

Facile Synthesis ◽

E Coli ◽

Surface Enhanced ◽

Surface Enhanced Raman ◽

Enhanced Raman Scattering

A green microwave procedure was developed to synthesize highly surface-enhanced Raman scattering (SERS) active silver nanoparticles (AgNPs) with a resonance Rayleigh scattering (RRS) peak at 450 nm, and a new SERS enhancement mechanism was proposed.

Download Full-text