scholarly journals Current Prevalence of Oral Helicobacter pylori among Japanese Adults Determined Using a Nested Polymerase Chain Reaction Assay

Pathogens ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 10
Author(s):  
Ryoko Nagata ◽  
Tatsuya Ohsumi ◽  
Shoji Takenaka ◽  
Yuichiro Noiri
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Infection Prevalence ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Lower Incisor ◽  
Characteristic Distribution ◽  
Japanese Adults ◽  
Polymerase Chain ◽  
H Pylori

In Japan, gastric Helicobacter pylori infection prevalence has markedly decreased with socioeconomic development. We aimed to investigate the prevalence of oral H. pylori in Japanese adults in 2020 by sex, age, sampling site, and medical history. Unstimulated saliva, supragingival biofilm, and tongue coating were obtained from 88 subjects–with no complaints of upper digestive symptoms–attending a dentist’s office for dental check-up or disorders. Supragingival biofilm was collected from the upper incisors, lower incisors, upper right molars and lower left molars to analyze the characteristic distribution. Oral H. pylori was detected using nested polymerase chain reaction. Oral H. pylori prevalence did not statistically differ by sex or age. Supragingival biofilm (30.7%) was the most common oral H. pylori niche; it was also detected in 4.5% of saliva and 2.3% of tongue samples. The lower incisor was the most common site among the supragingival biofilm samples, followed by the upper incisors, lower left molars, and upper right molars. Oral H. pylori DNA was frequently detected in patients with a history of gastric H. pylori infection. Oral H. pylori has a characteristic distribution independent of sex and age, suggesting that it is part of the normal microflora in the adult oral cavity.

scholarly journals Detection of Helicobacter pylori using nested polymerase chain reaction in gastric biopsy samples

2008 ◽  
Vol 23 (3) ◽  
pp. 243-245 ◽  
Author(s):  
Divya Mahajan ◽  
Anju Jain ◽  
Varsha Singh ◽  
A. K. Jain ◽  
G. R. K. Rao ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Prevalence of the Helicobacter pylori babA2 Gene in Children Mainly Depends on the PCR Primer Set Used

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Anja Šterbenc ◽  
Maja M. Lunar ◽  
Matjaž Homan ◽  
Boštjan Luzar ◽  
Nina Zidar ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Diagnostic Tool ◽  
Clinical Relevance ◽  
Detection Methods ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Primer Sets ◽  
H Pylori ◽  
Pcr Primer

Various polymerase chain reaction- (PCR-) based methods with varying positivity rates were designed to detect the Helicobacter pylori babA2 gene. To compare different primer sets, babA2 prevalence was determined in 279 H. pylori-positive pediatric samples using the 832 bp, 139 bp, and 271 bp PCR primer sets, resulting in 34.0%, 51.3%, and 79.6% prevalence of the babA2 gene, respectively. The babA2 status determined using the 832 bp and 139 bp PCR primer sets significantly correlated with bacterial density and activity of inflammation, whereas no such correlations were found using the 271 bp PCR primer set. The 139 and 832 bp PCR primer sets concordantly detected the babA2 gene in 93 cases; however, in comparison to the 832 bp PCR primer set, the 139 bp PCR primer set detected additional 50 babA2 cases, whereas only two 832 bp positive cases were missed. The 271 bp PCR primer set missed 32 babA2 cases that were 832 bp and/or 139 bp PCR positive, but tested solely positive in 109 cases. Interestingly, cloning of a subset of 271 bp PCR positive samples revealed amplification of the babA/B gene chimera. Hence, in our opinion, the 271 bp PCR protocol is not a reliable diagnostic tool for detecting the babA2 gene in children. Our results reaffirm previous observations that the use of certain babA2 PCR primer sets can significantly impact estimation of the prevalence and clinical relevance of the H. pylori babA2 gene in children, suggesting babA2 detection methods should be carefully selected.

scholarly journals Detection of Helicobacter pylori glmM gene in bovine milk using Nested polymerase chain reaction

2015 ◽  
Vol 8 (7) ◽  
pp. 913-917 ◽  
Author(s):  
Eyman Y. Osman ◽  
A. M. S. El-Eragi ◽  
Abuobeida M. Musa ◽  
Salma B. El-Magboul ◽  
Magdi B. A/Rahman ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Clarithromycin-resistant Helicobacter pylori in Recife, Brazil, directly identified from gastric biopsies by polymerase chain reaction

2010 ◽  
Vol 47 (4) ◽  
pp. 379-382 ◽  
Author(s):  
Ana Kelly Lins ◽  
Roberto A Lima ◽  
Marcelo Magalhães
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Susceptibility Testing ◽  
Point Mutations ◽  
Chain Reaction ◽  
Clarithromycin Resistance ◽  
Polymerase Chain ◽  
Pre Treatment ◽  
H Pylori ◽  
Phenotypic Methods

CONTEXT: Clarithromycin is the most effective drug used in the eradication of infection by Helicobacter pylori. Due to worldwide increase in resistance, pre-treatment susceptibility testing for clarithromycin is recommended. OBJECTIVES: To evaluate the prevalence of clarithromycin resistance of H. pylori in Recife, a city in Northeast Brazil. METHODS: From January 2006 to December 2007, 114 gastric biopsy samples positive for H. pylori at culture were directly assayed by polymerase chain reaction (PCR) to detect the most frequent point mutations involved in clarithromycin resistance. Results were compared with those obtained by Etests. RESULT: Molecular and phenotypic methods showed 111 (97.4%) susceptible or resistant concordant results. PCR detected 3 (2.6%) biopsy specimens with H. pylori-resistant genotypes, which were misdiagnosed as susceptible by Etests. In Recife, based on PCR results, primary clarithromycin resistance was found in 15 (16.5%) patients, prevalence close to that observed in Southeast Brazil. Resistance increased to 52% among previously treated patients. The point mutation A2143G was present in 20 (71.4%) of specimens and A2142G, in 8 (28.6%) of specimens. A2142C was not found. CONCLUSION: In Recife, the prevalence of primary clarithromycin resistance, 16.5%, showed the need for pretreatment susceptibility testing in H. pylori infections.

scholarly journals Refluks Helicobacter pylori di mukosa hidung penderita rinosinusitis kronik disertai refluks laringofaring

2019 ◽  
Vol 48 (2) ◽  
pp. 148
Author(s):  
Sinta Sari Ratunanda ◽  
Billy Talakua ◽  
Teti Madiadipoera ◽  
Thaufiq Boesoirie ◽  
Ratna Anggraeni ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Real Time ◽  
Chronic Rhinosinusitis ◽  
Nasal Mucosa ◽  
Nasal Discharge ◽  
Chain Reaction ◽  
Qrt Pcr ◽  
Polymerase Chain ◽  
H Pylori

Latar belakang: Rinosinusitis kronik masih menjadi problema di seluruh dunia. Faktor yang berasosiasi dengan Rinosinusitis Kronik (RSK) diduga multifaktorial, salah satunya adalah refluks laringofaring (RLF). Isi refluks cairan lambung antara lain adalah bakteri Helicobacter pylori (H. pylori) yang dengan patomekanisme refluks, diduga dapat mencapai mukosa laringofaring bahkan sampai mukosa sinonasal, dan menyebabkan RSK. Tujuan: Mendeteksi  H. pylori di mukosa hidung akibat refluks pada penderita RSK disertai RLF. Bila terdeteksi H. pylori, tata laksana harus lebih komprehensif, sehingga diharapkan RSK menjadi terkontrol. Metode: Penelitian deskriptif untuk mengetahui ada tidaknya H. pylori di mukosa sinonasal penderita RSK dengan RLF. Deteksi H. pylori menggunakan teknik quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) dari bahan penyikatan mukosa hidung. Hasil: Didapatkan 86 orang penderita RSK disertai RLF, terdiri dari 30 (35%) pasien laki-laki dan 56 (65,0%) pasien wanita, dengan rerata usia 43,25±6,30 tahun. Keluhan RSK terbanyak adalah hidung tersumbat dengan skor VAS > 7 sebesar 76,8%. Skor nasoendoskopi RSK terbesar pada skor 2 untuk edema mukosa sebesar 65,3% dan skor 2 untuk sekret hidung sebesar 58,2%. Rata-rata skor gejala refluks (SGR) adalah 26,43±4,03 dan rata-rata total skor temuan refluks (STR) adalah 11,28±1,21. Hasil pemeriksaan deteksi H. pylori dengan qRT-PCR, 100% tidak menemukan H. pylori dari penyikatan mukosa hidung. Kesimpulan: Refluks berupa H. pylori tidak ditemukan pada mukosa hidung  penderita RSK disertai RLF. Penelitian lebih lanjut diperlukan dengan menggunakan gabungan beberapa metode pemeriksaan  bersamaan untuk deteksi H. pylori akibat refluks di mukosa sinonasal  penderita RSK disertai RLF.  Background: Chronic rhinosinusitis is presently still a worldwide problem. Assosiating factors  to chronic rhinosinusitis (CRS) are multifactorial, one of them is laryngopharyngeal reflux (LPR). The gastric juice contains Helicobacter pylori (H. pylori), which by pathologic reflux could reach laryngopharyngeal and sinonasal area causing CRS. Purpose: To detect H. pylori in nasal mucosa caused by reflux, which suspected of causing CRS with LPR disease. Should H. pylori be found in nasal mucosa, the management of the disease must be comprehensive to enable  controlling CRS. Methods: A descriptive study to detect H. pylori in nasal mucosa CRS with LPR patients, using Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) through nasal brushing. Results: Eighty-six CRS with LPR patients as study objects consisted of 30 (35%) male, and 56 (65%) female, the age mean was 43.25±6.3 years old. Visual Analoque Scale (VAS) score for nasal obstruction more than 7 was the highest complaint (76.8%). Nasal endoscopic score of mucosal edema (65.3%) and nasal discharge (58,2%) had score 2. The average total score reflux symptom index (RSI) was 26.43±4.03 and the total score reflux finding score (RFS) was 11.28±1.21. H. pylori detection found negative 100% in CRS with LPR specimens. Conclusion: This study did not find reflux containing H. pylori in nasal mucosa of CRS with LPR patients.    Suggesting further study using simultaneously several methods to detect H. pylori in nasal mucosa  CRS with LPR patients.

Absence ofHelicobacter pyloriin healthy laryngeal mucosa

2011 ◽  
Vol 126 (2) ◽  
pp. 196-199 ◽  
Author(s):  
I Pajić-Penavić ◽  
D Đanić ◽  
S Maslovara ◽  
K Gall-Trošelj
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Breath Test ◽  
Gastrointestinal Disease ◽  
Urea Breath Test ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Laryngeal Mucosa ◽  
Polymerase Chain

AbstractObjectives:To evaluate the presence ofHelicobacter pyloriin healthy laryngeal mucosa.Design:Prospective analysis ofHelicobacter pyloricolonisation in healthy laryngeal mucosa, using the13C urea breath test and polymerase chain reaction analysis.Subjects:Twenty randomly chosen men (28–78 years) without laryngeal pathology or gastrointestinal disease were investigated. All subjects were scheduled for elective operative procedures, under general, endotracheal anaesthesia. Cytobrush samples were taken forHelicobacter pyloriDNA detection. Nested polymerase chain reaction testing was performed on DNA solutions using two primer pairs from the urease A gene of theHelicobacter pylorigenome. The13C urea breath test was performed on two exhalation samples.Results:Eight (40 per cent) of the subjects were positive for urease on urea breath testing; none were positive forHelicobacter pyloriDNA on polymerase chain reaction testing.Conclusion:Based on these results, we do not considerHelicobacter pylorito be a normal constituent of healthy laryngeal microflora.

Molecular Identification of Helicobacter pylori and IceA Genes Frequency from Dental Plaques Isolated from People Using PCR Method

2020 ◽  
Author(s):  
Zahra Salari ◽  
Atefeh Ranjkesh ◽  
Emad Behboudi
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Chain Reaction ◽  
Ulcer Disease ◽  
Two Samples ◽  
Pcr Method ◽  
Dental Plaques ◽  
Polymerase Chain ◽  
H Pylori ◽  
Development And Evolution

Background and Aims: Helicobacter pylori (H. pylori) is a gram-negative, microaerophilic, spiral-shaped flagellated bacterium that is urease, catalase and oxidase positive. One of its pathogenicity factors is the iceA gene. H. pylori has recently been recognized as a genetic indicator for the development and evolution of duodenal ulcer disease in the East. This study aimed to determine the presence of this bacterium in gingival plaques in non-endocrine patients in Bojnourd city, and the polymerase chain reaction technique examined the percentage of iceA gene. Materials and Methods: A total of 100 samples of dental plaque were taken and transferred to a tube that has been physiologically placed. After DNA extraction, primer design was performed, and then the polymerase chain reaction was performed for the whole sample. Results: Of 100 samples examined in this study, two samples of H. pylori were positive (2%), and the frequency of the iceA gene of two samples was positive (100%). Conclusion: In the Bojnord city, the frequency of iceA gene in people is high, and the frequency of H. pylori in tooth plaques is low. Also, iceA gene can be considered as an indicator for predicting the contamination and risk of H. pylori infection in the region. To confirm the results, more molecular studies are required in other populations.

Detection of Helicobacter pylori in various oral lesions by nested polymerase chain reaction (PCR)

2007 ◽  
Vol 27 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Marinka Mravak-Stipetić ◽  
Koraljka Gall-Tros̆elj ◽  
Josip Lukac̆ ◽  
Zvonko Kusić ◽  
Kres̆imir Pavelić ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Oral Lesions ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Is the polymerase chain reaction or cure of Helicobacter pylori infection of help in the differential diagnosis of early gastric mucosa-associated lymphatic tissue lymphoma?

1997 ◽  
Vol 15 (3) ◽  
pp. 1104-1109 ◽  
Author(s):  
B Rudolph ◽  
E Bayerdörffer ◽  
M Ritter ◽  
S Müller ◽  
C Thiede ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Differential Diagnosis ◽  
Gastric Mucosa ◽  
Malt Lymphoma ◽  
Complete Regression ◽  
Lymphatic Tissue ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
H Pylori

PURPOSE The differential diagnosis of early gastric mucosa-associated lymphatic tissue (MALT) lymphoma based on Helicobacter pylori gastritis may be difficult when lymphoepithelial lesions are not detected. The aim of the present study was to investigate the question whether the polymerase chain reaction (PCR) or cure of H pylori infection may be of help in this respect. PATIENTS AND METHODS Twenty patients with suspected low-grade gastric MALT lymphomas were treated in a double-blinded, randomized, crossover trial with 2,250 mg of either amoxicillin or placebo, both in combination with omeprazole, for 14 days with the aim to cure H pylori infection. PCR was performed using primers specific for the CDR3 region to detect monoclonal B cells. RESULTS In five of 20 patients, MALT lymphomas were finally diagnosed. Three of these five patients went into complete remission, while two were referred to surgery. In the 15 patients with gastritis, complete regression was observed in all cases. With respect to PCR, monoclonal bands were detected in all four of the analyzed lymphoma patients before histology showed lymphoma. In addition, monoclonal bands were found in three patients with gastritis. In the patients with gastritis and monoclonal PCR, complete regression took longer as compared with the remaining 12 patients with polyclonal PCR and gastritis (P = .0209). Successful H pylori eradication was associated with earlier diagnosis of the MALT lymphoma (P = .0237). CONCLUSION CDR3-PCR may be of help in the differential diagnosis of early gastric MALT lymphoma. Furthermore, H pylori eradication may lead to earlier diagnosis.

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