scholarly journals Antibody Profile Comparison against MSP1 Antigens of Multiple Plasmodium Species in Human Serum Samples from Two Different Brazilian Populations Using a Multiplex Serological Assay

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1138
Author(s):  
Eliana Ferreira Monteiro ◽  
Carmen Fernandez-Becerra ◽  
Izilda Curado ◽  
Gerhard Wunderlich ◽  
Meire Ioshie Hiyane ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Plasmodium Species ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Plasmodium Malariae ◽  
Multiplex Assay ◽  
Reactivity Index ◽  
Malaria Surveillance ◽  
Serum Samples ◽  
Wide Geographic Distribution

Plasmodium malariae has a wide geographic distribution, but mainly at very low parasitemias and in co-infections, leading to an underestimated prevalence of this species. Studies for the detection of antibodies against Plasmodium recombinant proteins are increasingly used to map geographical distributions, seroprevalence and transmission intensities of malaria infection. However, no seroepidemiological survey using recombinant P. malariae proteins has been conducted in Brazil. This work evaluated the antibody response in serum samples of individuals from endemic regions of Brazil (the Amazon region and Atlantic Forest) against five recombinant proteins of P. malariae merozoite surface protein 1 (MSP1), and the MSP1 C-terminal portions of P. vivax and P. falciparum, in a multiplex assay. The positivity was 69.5% of samples recognizing at least one MSP1 recombinant protein. The mean of the Reactivity Index for the C-terminal portion of the P. falciparum was significantly higher compared to the other recombinant proteins, followed by the C-terminal of P. vivax and the N-terminal of P. malariae. Among the recombinant P. malariae proteins, the N-terminal of P. malariae showed the highest Reactivity Index alone. This study validates the use of the multiplex assay to measure naturally acquired IgG antibodies against Plasmodium MSP1 proteins and demonstrate that these proteins are important tools for seroepidemiological surveys and could be used in malaria surveillance.

scholarly journals Recombinant proteins of Plasmodium malariae merozoite surface protein 1 (PmMSP1): Testing immunogenicity in the BALB/c model and potential use as diagnostic tool

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0219629 ◽  
Author(s):  
Yelina B. Elizardez ◽  
Wesley L. Fotoran ◽  
Andrés J. Galisteo Junior ◽  
Izilda Curado ◽  
Norival Kesper Junior ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Diagnostic Tool ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Plasmodium Malariae ◽  
Merozoite Surface Protein 1 ◽  
Potential Use

scholarly journals Mosquito Bite-Induced Controlled Human Malaria Infection withPlasmodium vivaxorP. falciparumGenerates Immune Responses to Homologous and Heterologous Preerythrocytic and Erythrocytic Antigens

2018 ◽  
Vol 87 (3) ◽  
Author(s):  
Cysha E. Hall ◽  
Lisa M. Hagan ◽  
Elke Bergmann-Leitner ◽  
Donna M. Tosh ◽  
Jason W. Bennett ◽  
...  
Keyword(s):  
Malaria Infection ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Similar Proportion ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Content Type ◽  
Human Malaria ◽  
Before And After ◽  
Controlled Human Malaria Infection

ABSTRACTSeroepidemiological studies on the prevalence of antibodies to malaria antigens are primarily conducted on individuals from regions of endemicity. It is therefore difficult to accurately correlate the antibody responses to the timing and number of prior malaria infections. This study was undertaken to assess the evolution of antibodies to the dominant surface antigens ofPlasmodium vivaxandP. falciparumfollowing controlled human malaria infection (CHMI) in malaria-naive individuals. Serum samples from malaria-naive adults, collected before and after CHMI with eitherP. vivax(n= 18) orP. falciparum(n= 18), were tested for the presence of antibodies to the circumsporozoite protein (CSP) and the 42-kDa fragment of merozoite surface protein 1 (MSP-142) ofP. vivaxandP. falciparumusing an enzyme-linked immunosorbent assay (ELISA). Approximately 1 month following CHMI with eitherP. vivaxorP. falciparum, >60% of subjects seroconverted to homologous CSP and MSP-1. More than 50% of the subjects demonstrated reactivity to heterologous CSP and MSP-142, and a similar proportion of subjects remained seropositive to homologous MSP-142>5 months after CHMI. Computational analysis provides insight into the presence of cross-reactive responses. The presence of long-lived and heterologous reactivity and its functional significance, if any, need to be taken into account while evaluating malaria exposure in field settings.

Protein–protein interaction studies reveal the Plasmodium falciparum merozoite surface protein-1 region involved in a complex formation that binds to human erythrocytes

2018 ◽  
Vol 475 (6) ◽  
pp. 1197-1209 ◽  
Author(s):  
Gourab Paul ◽  
Arunaditya Deshmukh ◽  
Bishwanath Kumar Chourasia ◽  
Md Kalamuddin ◽  
Ashutosh Panda ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Recombinant Proteins ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Human Erythrocytes ◽  
Proteolytic Processing ◽  
Protein Protein Interaction ◽  
Falciparum Merozoite ◽  
Vaccine Candidate Antigen ◽  
Serological Studies

Plasmodium falciparum merozoite surface protein (PfMSP) 1 has been studied extensively as a vaccine candidate antigen. PfMSP-1 undergoes proteolytic processing into four major products, such as p83, p30, p38, and p42, that are associated in the form of non-covalent complex(s) with other MSPs. To delineate MSP1 regions involved in the interaction with other MSPs, here we expressed recombinant proteins (PfMSP-165) encompassing part of p38 and p42 regions and PfMSP-119. PfMSP-165 interacted strongly with PfMSP-3, PfMSP-6, PfMSP-7, and PfMSP-9, whereas PfMSP-119 did not interact with any of these proteins. Since MSP-1 complex binds human erythrocytes, we examined the ability of these proteins to bind human erythrocyte. Among the proteins of MSP-1 complex, PfMSP-6 and PfMSP-9 bound to human erythrocytes. Serological studies showed that PfMSP-165 was frequently recognized by sera from malaria endemic regions, whereas this was not the case for PfMSP-119. In contrast, antibodies against PfMSP-119 showed much higher inhibition of merozoite invasion compared with antibodies against the larger PfMSP-165 fragment. Importantly, anti-PfMSP-119 antibodies recognized both recombinant proteins, PfMSP-119 and PfMSP-165; however, anti-PfMSP-165 antibody failed to recognize the PfMSP-119 protein. Taken together, these results demonstrate that PfMSP-1 sequences upstream of the 19 kDa C-terminal region are involved in molecular interactions with other MSPs, and these sequences may probably serve as a smoke screen to evade antibody response to the membrane-bound C-terminal 19 kDa region.

Immunogenicity of Plasmodium vivax merozoite surface protein-9 recombinant proteins expressed in E. coli

Vaccine ◽  
2004 ◽  
Vol 22 (15-16) ◽  
pp. 2023-2030 ◽  
Author(s):  
Joseli Oliveira-Ferreira ◽  
Esmeralda Vargas-Serrato ◽  
John W Barnwell ◽  
Alberto Moreno ◽  
Mary R Galinski
Keyword(s):  
Plasmodium Vivax ◽  
Recombinant Proteins ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
E Coli

scholarly journals Humoral Responses to Plasmodium falciparum Blood-Stage Antigens and Association with Incidence of Clinical Malaria in Children Living in an Area of Seasonal Malaria Transmission in Burkina Faso, West Africa

2007 ◽  
Vol 76 (2) ◽  
pp. 759-766 ◽  
Author(s):  
Issa Nebie ◽  
Amidou Diarra ◽  
Alphonse Ouedraogo ◽  
Issiaka Soulama ◽  
Edith C. Bougouma ◽  
...  
Keyword(s):  
Malaria Transmission ◽  
Malaria Vaccine ◽  
Vaccine Development ◽  
Malaria Incidence ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Clinical Malaria ◽  
Blood Stage ◽  
Igg Subclass ◽  
Malaria Surveillance

ABSTRACT There is longstanding evidence that immunoglobulin G (IgG) has a role in protection against clinical malaria, and human antibodies of the cytophilic subclasses are thought to be particularly critical in this respect. In this cohort study, 286 Burkinabè children 6 months to 15 years old were kept under malaria surveillance in order to assess the protective role of antibody responses against four antigens which are currently being evaluated as vaccine candidates: apical membrane antigen 1 (AMA1), merozoite surface protein 1-19 (MSP1-19), MSP3, and glutamate-rich protein (GLURP). Total IgG, IgM, and IgG subclass responses were measured just before the malaria transmission season. The incidence of malaria was 2.4 episodes per child year of risk. After adjusting for the confounding effects of age, the level of total IgG to GLURP was strongly associated with reduced malaria incidence (incidence rate ratio associated with a doubling of total IgG, 0.79; 95% confidence interval, 0.66 to 0.94; P = 0.009.); there was a borderline statistically significant association between the level of total IgG to MSP3 and malaria incidence and no evidence of an association for total IgG to AMA1 and to MSP1-19. Of the IgG subclass responses studied, only IgG3 and IgG4 against GLURP and IgG1 against AMA1 were associated with reduced risk of clinical malaria. There was no evidence of an interaction between responses to AMA1 and baseline parasitemia in their effects on malaria incidence. Currently included in malaria vaccine formulations for clinical trials in humans, these blood-stage antigens, AMA1 and GLURP, offer good prospects for malaria vaccine development.

scholarly journals Usefulness of Seminested Multiplex PCR in Surveillance of Imported Malaria in Spain

1999 ◽  
Vol 37 (10) ◽  
pp. 3260-3264 ◽  
Author(s):  
J. M. Rubio ◽  
A. Benito ◽  
P. J. Berzosa ◽  
J. Roche ◽  
S. Puente ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
National Health System ◽  
Central Africa ◽  
Plasmodium Malariae ◽  
Reference Laboratory ◽  
Mixed Infections ◽  
Malaria Surveillance ◽  
Serum Samples ◽  
African Countries ◽  
West Central

The use of a new PCR-based method for the diagnosis of malaria in the Spanish Malaria Reference Laboratory has promoted an increase in confirmed cases of malaria. From August 1997 to July 1998, a total of 192 whole-blood samples and 71 serum samples from 168 patients were received from the hospitals of the Spanish National Health System. Most of the patients came from west-central African countries (85%). This molecular method showed more sensitivity and specificity than microscopy, detecting 12.4% more positive samples than microscopy and 13% of mixed infections undetectable by Giemsa stain. Plasmodium falciparum was the main species detected, with 68% of the total positive malaria cases, followed by Plasmodium malariae(29%), Plasmodium vivax (14%), and Plasmodium ovale (7%), including mixed infections in all cases. This report consists of the first wide, centralized survey of malaria surveillance in Spain. The reference laboratory conducted the analysis of all imported cases in order to detect trends in acquisition. The use of a seminested multiplex PCR permitted confirmation of the origins of the infections and the Plasmodium species involved and confirmation of the effectiveness of drug treatments. This PCR also allowed the detection of the presence in Spain of primaquine-tolerantP. vivax strains from west-central Africa, as well as the detection of a P. falciparum infection induced by transfusion.

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