scholarly journals Molecular Detection and Characterization of Borrelia garinii (Spirochaetales: Borreliaceae) in Ixodes nipponensis (Ixodida: Ixodidae) Parasitizing a Dog in Korea

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 289 ◽  
Author(s):  
Seung-Hun Lee ◽  
Youn-Kyoung Goo ◽  
Paul John L. Geraldino ◽  
Oh-Deog Kwon ◽  
Dongmi Kwak
Keyword(s):  
Molecular Detection ◽  
Single Gene ◽  
Protein A ◽  
Intergenic Spacer ◽  
Surface Protein ◽  
23S Rrna ◽  
Borrelia Garinii ◽  
Intergenic Spacer Region ◽  
Individual Level ◽  
Pcr Targeting

The present study aimed to detect and characterize Borrelia spp. in ticks attached to dogs in Korea. Overall, 562 ticks (276 pools) attached to dogs were collected and tested for Borrelia infection by PCR targeting the 5S-23S rRNA intergenic spacer region (rrf-rrl). One tick larva (pool level, 0.4%; individual level, 0.2%) was confirmed by sequencing Borrelia garinii, a zoonotic pathogen. For molecular characterization, the outer surface protein A (ospA) and flagellin genes were analyzed. Phylogenetic ospA analysis distinguished B. garinii from B. bavariensis, which has been recently identified as a novel Borrelia species. On the other hand, phylogenetic analysis showed that single gene analysis involving rrf-rrl or flagellin was not sufficient to differentiate B. garinii from B. bavariensis. In addition, the B. garinii-infected tick was identified as Ixodes nipponensis by sequencing according to mitochondrial 16S rRNA and the second transcribed spacer region. To our knowledge, this is the first study to report the molecular detection of B. garinii in I. nipponensis parasitizing a dog in Korea. Continuous monitoring of tick-borne pathogens in ticks attached to animals is required to avoid disease distribution and possible transmission to humans.

scholarly journals Molecular Identification of Borrelia spp. from Ticks in Pastures nearby Livestock Farms in Korea

Insects ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1011
Author(s):  
Haeseung Lee ◽  
Seung-Hun Lee ◽  
SungShik Shin ◽  
Dongmi Kwak
Keyword(s):  
Tick Species ◽  
Pathogenic Bacteria ◽  
Intergenic Spacer ◽  
23S Rrna ◽  
Haemaphysalis Longicornis ◽  
Intergenic Spacer Region ◽  
Close Relationship ◽  
Haemaphysalis Flava ◽  
Ixodes Ticks ◽  
Pcr Targeting

Ticks are vectors that spread pathogenic bacteria, viruses, and protozoa. As the number of ticks increases due to climate change, the importance of the study of tick-borne pathogens has also increased. This study was conducted to investigate the distribution of the major tick species causing Lyme borreliosis and regional differences in the prevalence of Borrelia spp. by tick species. Borrelia infection was confirmed not only in Ixodes ticks, which are the major vectors of Borrelia spp., but also in Haemaphysalis and Amblyomma ticks. PCR targeting the 5S-23S rRNA intergenic spacer region (rrf-rrl) was performed to confirm Borrelia positivity. A total of 6102 ticks (736 pools) were tested, and the proportion was Haemaphysalis longicornis nymphs and adults at 69.2%, Haemaphysalis flava nymphs and adults at 13.9%, Haemaphysalis spp. larva at 14.3%, Ixodes nipponensis at 0.8%, and Amblyomma testudinarium at 1.9%. Ixodes nipponensis showed the highest minimum infection rate (MIR: 34.00; 17 pools/50 ticks) for Borrelia spp., followed by A. testudinarium (MIR: 0.88), and H. longicornis (MIR: 0.05). In particular, to our knowledge Borrelia infection was first confirmed in A. testudinarium in Korea. As a result of phylogenetic analysis, all sequences were grouped with Borreliaafzelii isolates and showed a close relationship with high identity. Considering that B. afzelii causes infectious zoonotic diseases, continuous monitoring and attention are needed, although it has a low prevalence in this study.

scholarly journals Longitudinal monitoring of the dynamics of infections due to Bartonella species in UK woodland rodents

2001 ◽  
Vol 126 (2) ◽  
pp. 323-329 ◽  
Author(s):  
R. J. BIRTLES ◽  
S. M. HAZEL ◽  
M. BENNETT ◽  
K. BOWN ◽  
D. RAOULT ◽  
...  
Keyword(s):  
Sequence Comparison ◽  
Intergenic Spacer ◽  
23S Rrna ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Bartonella Species ◽  
Intergenic Spacer Region ◽  
Specific Pcr ◽  
Time Specific ◽  
Pcr Targeting

Blood samples were repeatedly collected from 12 sympatric woodland rodents over a 12-month period and DNA extracts from each were incorporated into a bartonella-specific PCR targeting a fragment of the 16S/23S rRNA intergenic spacer region (ISR). The composition of each amplicon was analysed using restriction enzyme analysis (REA) and base sequence comparison. Bartonella DNA was detected in 70 of 109 samples. Eleven samples contained DNA derived from more than one strain. Sequence analysis of 62 samples found 12 sequence variants (ISR genotypes) that were provisionally assigned to 5 different species, 2 of which were newly recognized. Up to five different species were detected in each animal. On about two-thirds of occasions, a species detected 1 month was not there the next, but never was a genotype superseded by another of the same species. However, a genotype could be re-encountered months later in the same animal, even if interim samples contained other genotypes. Our results suggest that although most animals are bacteraemic most of the time, specific infections are often superseded and that a complex and dynamic epidemiology of bartonella bacteraemias exists in woodland rodents.

Genomic fingerprinting ofFrankiamicrosymbionts fromCeanothuscopopulations using repetitive sequences and polymerase chain reactions

1999 ◽  
Vol 77 (9) ◽  
pp. 1220-1230 ◽  
Author(s):  
Soon-Chun Jeong ◽  
David D Myrold
Keyword(s):  
Dna Sequencing ◽  
Dna Analysis ◽  
Repetitive Sequences ◽  
Intergenic Spacer ◽  
Rrna Genes ◽  
23S Rrna ◽  
Genomic Fingerprinting ◽  
Chain Reactions ◽  
Intergenic Spacer Region ◽  
Polymerase Chain

Specificity between Ceanothus species and their microsymbionts, Frankia, were investigated with nodules collected from three geographically separated copopulations of Ceanothus species. Nodules were analyzed using DNA sequencing and repetitive sequence polymerase chain reaction (rep-PCR) techniques. DNA sequencing of the intergenic spacer region between 16S and 23S rRNA genes suggested that Ceanothus-microsymbiotic Frankia are closely related at the intraspecific level. Diversity of the microsymbionts was further analyzed by genomic fingerprinting using repetitive sequences and PCR. A newly designed direct repeat (DR) sequence and a BOX sequence were used as PCR primers after justification that these primers can generate Frankia-specific fingerprints from nodule DNA. Analysis of the nodules using BOX- and DR-PCR showed that Ceanothus-microsymbiotic Frankia exhibited less diversity within each copopulation than among copopulations. These data suggested that geographic separation plays a more important role for divergence of Ceanothus-microsymbiotic Frankia than host plant.Key words: Frankia, Ceanothus, rep-PCR, diversity.

Differentiation of group VIII Spiroplasma strains with sequences of the 16S–23S rDNA intergenic spacer region

2004 ◽  
Vol 50 (12) ◽  
pp. 1061-1067 ◽  
Author(s):  
Laura B Regassa ◽  
Kimberly M Stewart ◽  
April C Murphy ◽  
Frank E French ◽  
Tao Lin ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Analytical Models ◽  
23S Rrna ◽  
Rrna Gene ◽  
Group Viii ◽  
Similarity Coefficients ◽  
Intergenic Spacer Region ◽  
Intergenic Sequences ◽  
The Stability ◽  
The 16S Rrna Gene

Spiroplasma species (Mollicutes: Spiroplasmataceae) are associated with a wide variety of insects, and serology has classified this genus into 34 groups, 3 with subgroups. The 16S rRNA gene has been used for phylogenetic analysis of spiroplasmas, but this approach is uninformative for group VIII because the serologically distinct subgroups generally have similarity coefficients >0.990. Therefore, we investigated the utility of the 16S–23S rRNA spacer region as a means to differentiate closely related subgroups or strains. We generated intergenic sequences and detailed serological profiles for 8 group VIII Spiroplasma strains. Sequence analyses using Maximum Parsimony, Neighbor Joining, and Maximum Likelihood placed the strains into 2 clades. One clade consisted of strains BARC 2649 and GSU5367. The other clade was divided into clusters containing representatives of the 3 designated group VIII subgroups (EA-1, DF-1, and TAAS-1) and 3 previously unclassified strains. The stability of the positions of the strains in various analytical models and the ability to provide robust support for groupings tentatively supported by serology indicates that the 16S–23S intergenic rDNA sequence will prove useful in intragroup analysis of group VIII spiroplasmas.Key words: Mollicutes, Spiroplasma, phylogeny, Tabanidae.

A putative transposase gene in the 16S–23S rRNA intergenic spacer region of Mycoplasma imitans

Microbiology ◽  
2004 ◽  
Vol 150 (4) ◽  
pp. 1023-1029 ◽  
Author(s):  
Ryô Harasawa ◽  
David G. Pitcher ◽  
Ana S. Ramírez ◽  
Janet M. Bradbury
Keyword(s):  
Relative Size ◽  
Intergenic Spacer ◽  
Its Region ◽  
23S Rrna ◽  
Mycoplasma Species ◽  
Insertion Element ◽  
Transposase Gene ◽  
Intergenic Spacer Region ◽  
Pcr Products ◽  
Type Strains

Examination of the nucleotide sequences of the 16S–23S intergenic transcribed spacer (ITS) region of Mycoplasma imitans and Mycoplasma gallisepticum identified a putative transposase gene located only in the ITS of M. imitans, which can be used as a genetic marker to distinguish these two species. The relative size of the PCR products of the ITS region allowed a clear distinction to be made between strains of M. imitans and M. gallisepticum, both of which could be readily discriminated from the type strains of all the other recognized avian Mycoplasma species. In addition, the putative transposase gene assigned in the ITS of M. imitans was shown to include a sequence homologous to that of the P75 gene of M. gallisepticum. This is believed to be the first description of an insertion element in the rRNA operon region of a mycoplasma species.

scholarly journals Characteristics of the 16S-23S rRNA Intergenic Spacer Region of Mycoplasma haemomuris, Previously Classified as `Haemobartonella muris'

2002 ◽  
Vol 64 (12) ◽  
pp. 1161-1164 ◽  
Author(s):  
Ryô HARASAWA ◽  
Makoto KAWAHARA ◽  
Yasuko RIKIHISA
Keyword(s):  
Intergenic Spacer ◽  
23S Rrna ◽  
Intergenic Spacer Region

scholarly journals Sequence analysis of 16S rRNA gene and 16S–23S rRNA gene intergenic spacer region for differentiation of probioticsLactobacillus strains isolated from the gastrointestinal tract of chicken

2008 ◽  
Vol 58 (1) ◽  
pp. 133-140 ◽  
Author(s):  
Chin Mei Lee ◽  
Chin Chin Sieo ◽  
Clemente Michael Vui Ling Wong ◽  
Norhani Abdullah ◽  
Yin Wan Ho
Keyword(s):  
Gastrointestinal Tract ◽  
Sequence Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Intergenic Spacer ◽  
23S Rrna ◽  
Rrna Gene ◽  
Intergenic Spacer Region ◽  
23S Rrna Gene

scholarly journals 16S–23S rRNA Gene Intergenic Spacer Region Variability Helps Resolve Closely Related Sphingomonads

2016 ◽  
Vol 7 ◽  
Author(s):  
Sima Tokajian ◽  
Nahla Issa ◽  
Tamara Salloum ◽  
Joe Ibrahim ◽  
Maya Farah
Keyword(s):  
Intergenic Spacer ◽  
23S Rrna ◽  
Rrna Gene ◽  
Intergenic Spacer Region ◽  
23S Rrna Gene

scholarly journals A diagnostic polymerase chain reaction forMycoplasma iowaeusing primers located in the intergenic spacer region and the 23S rRNA gene

2012 ◽  
Vol 41 (3) ◽  
pp. 317-322 ◽  
Author(s):  
Ana S. Ramírez ◽  
Cynthia Dare ◽  
Christine A. Yavari ◽  
Janet M. Bradbury
Keyword(s):  
Polymerase Chain Reaction ◽  
Intergenic Spacer ◽  
23S Rrna ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Intergenic Spacer Region ◽  
23S Rrna Gene ◽  
Polymerase Chain
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