scholarly journals Resveratrol-Loaded Lipid-Core Nanocapsules Modulate Acute Lung Inflammation and Oxidative Imbalance Induced by LPS in Mice

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 683
Author(s):  
Maria Talita Pacheco de Oliveira ◽  
Diego de Sá Coutinho ◽  
Sílvia Stanisçuaski Guterres ◽  
Adriana Raffin Pohlmann ◽  
Patrícia Machado Rodrigues e Silva ◽  
...  
Keyword(s):  
Lung Injury ◽  
Pulmonary Inflammation ◽  
Pharmacological Therapy ◽  
Free Form ◽  
Therapeutic Effects ◽  
Acute Lung Inflammation ◽  
Lung Injury Model ◽  
Anti Inflammatory ◽  
Pre Treatment ◽  
Antioxidant Effects

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are inflammatory and oxidative imbalance lung conditions with no successful pharmacological therapy and a high mortality rate. Resveratrol (RSV) is a plant-derived stilbene that presents anti-inflammatory and antioxidant effects. However, its therapeutic application remains limited due to its poor bioavailability, which can be solved by the use of nanocarriers. Previously, we demonstrated that nanoencapsulated RSV (RSV-LNC) pre-treatment, performed 4 h before lipopolysaccharide (LPS) stimulation in mice, increased its anti-inflammatory properties. In this study, we evaluated the anti-inflammatory and antioxidant effects, and lung distribution of RSV-LNCs administered therapeutically (6 h post LPS exposure) in a lung injury mouse model. The results showed that RSV-LNCs posttreatment improved lung function and diminished pulmonary inflammation. Moreover, RSV-LNC treatment enhanced the antioxidant catalase level together with a decrease in the oxidative biomarker in mouse lungs, which was accompanied by an increase in pulmonary Nrf2 antioxidant expression. Finally, the presence of RSV in lung tissue was significantly detected when mice received RSV-LNCs but not when they received RSV in its free form. Together, our results confirm that RSV nanoencapsulation promotes an increase in RSV bioavailability, enhancing its therapeutic effects in an LPS-induced lung injury model.

The therapeutic effects of anti-oxidant and anti-inflammatory drug quercetin on aspiration-induced lung injury in rats

2013 ◽  
Vol 45 (2) ◽  
pp. 195-203 ◽  
Author(s):  
Mehmet Ziya Yilmaz ◽  
Aygul Guzel ◽  
Aysun Caglar Torun ◽  
Ali Okuyucu ◽  
Osman Salis ◽  
...  
Keyword(s):  
Lung Injury ◽  
Therapeutic Effects ◽  
Inflammatory Drug ◽  
Anti Oxidant ◽  
Anti Inflammatory

Investigation of anti-inflammatory effects of oxygen nanobubbles in a rat hydrochloric acid lung injury model

Nanomedicine ◽  
2020 ◽  
Vol 15 (27) ◽  
pp. 2647-2654
Author(s):  
Keisuke Yoshida ◽  
Yukihiro Ikegami ◽  
Shinju Obara ◽  
Keiko Sato ◽  
Masahiro Murakawa
Keyword(s):  
Acute Lung Injury ◽  
Mean Arterial Pressure ◽  
Hydrochloric Acid ◽  
Lung Injury ◽  
Arterial Pressure ◽  
Rat Model ◽  
Injury Model ◽  
Lung Injury Model ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Aim: To investigate the anti-inflammatory effect of oxygen nanobubbles (ONBs) in an acute lung injury rat model. Materials & methods: In a rat hydrochloric acid lung injury model, ONB fluid was administered intravenously in the ONB group (n = 6) and normal saline was administered in the control group (n = 6). 4 h later, arterial partial pressure of oxygen (PaO2), mean arterial pressure and plasma inflammatory cytokines were measured. Results: There were no significant differences in the PaO2, mean arterial pressure or TNF-α and IL-6 levels between the two groups. Conclusions: No anti-inflammatory effect could be confirmed at the present ONB dose in the rat model of acute lung injury.

scholarly journals Prophylactic and therapeutic treatment with the flavonone sakuranetin ameliorates LPS-induced acute lung injury

2017 ◽  
Vol 312 (2) ◽  
pp. L217-L230 ◽  
Author(s):  
Márcia Isabel Bittencourt-Mernak ◽  
Nathalia M. Pinheiro ◽  
Fernanda P. R. Santana ◽  
Marina P. Guerreiro ◽  
Beatriz M. Saraiva-Romanholo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Lung Injury ◽  
Lung Function ◽  
Lung Injury ◽  
Collagen Fiber ◽  
Pulmonary Inflammation ◽  
Fiber Formation ◽  
Therapeutic Effects ◽  
Therapeutic Treatment ◽  
Fiber Deposition

Sakuranetin is the main isolate flavonoid from Baccharis retusa ( Asteraceae) leaves and exhibits anti-inflammatory and antioxidative activities. Acute respiratory distress syndrome is an acute failure of the respiratory system for which effective treatment is urgently necessary. This study investigated the preventive and therapeutic effects of sakuranetin on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Animals were treated with intranasal sakuranetin 30 min before or 6 h after instillation of LPS. Twenty-four hours after ALI was induced, lung function, inflammation, macrophages population markers, collagen fiber deposition, the extent of oxidative stress, and the expression of matrix metalloprotease-9 (MMP-9), tissue inhibitor of MMP-9 (TIMP-1) and NF-κB were evaluated. The animals began to show lung alterations 6 h after LPS instillation, and these changes persisted until 24 h after LPS administration. Preventive and therapeutic treatment with sakuranetin reduced the neutrophils in the peripheral blood and in the bronchial alveolar lavage. Sakuranetin treatment also reduced macrophage populations, particularly that of M1-like macrophages. In addition, sakurnaetin treatment reduced keratinocyte-derived chemokines (IL-8 homolog) and NF-κB levels, collagen fiber formation, MMM-9 and TIMP-1-positive cells, and oxidative stress in lung tissues compared with LPS animals treated with vehicle. Finally, sakuranetin treatment also reduced total protein, and the levels of TNF-α and IL-1β in the lung. This study shows that sakuranetin prevented and reduced pulmonary inflammation induced by LPS. Because sakuranetin modulates oxidative stress, the NF-κB pathway, and lung function, it may constitute a novel therapeutic candidate to prevent and treat ALI.

Therapeutic Effects and Anti-inflammatory Mechanisms of Heparin on Acute Lung Injury in Rabbits

2008 ◽  
Vol 15 (7) ◽  
pp. 656-663 ◽  
Author(s):  
Meitang Wang ◽  
Jian He ◽  
Bin Mei ◽  
Xiuqiang Ma ◽  
Zhenglu Huo
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Therapeutic Effects ◽  
Anti Inflammatory

scholarly journals The therapeutic effects of traditional Chinese medicine Fusu agent in LPS-induced acute lung injury model rats

2018 ◽  
Vol Volume 12 ◽  
pp. 3867-3878 ◽  
Author(s):  
Peiyang Gao ◽  
Ziyi Zhao ◽  
Chuantao Zhang ◽  
Chunxia Wang ◽  
Kunlan Long ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Lung Injury ◽  
Therapeutic Effects ◽  
Injury Model ◽  
Lung Injury Model

scholarly journals Baicalin Liposome Alleviates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Inhibiting TLR4/JNK/ERK/NF-κB Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Yu Long ◽  
Yan Xiang ◽  
Songyu Liu ◽  
Yulu Zhang ◽  
Jinyan Wan ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Lung Injury Score ◽  
Therapeutic Effects ◽  
Dependent Manner ◽  
Scutellaria Baicalensis Georgi ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Novel Drug

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are challenging diseases with the high mortality in a clinical setting. Baicalin (BA) is the main effective constituent isolated from the Chinese medical herb Scutellaria baicalensis Georgi, and studies have proved that it has a protective effect on ALI induced by lipopolysaccharide (LPS) due to the anti-inflammatory efficacy. However, BA has low solubility which may limit its clinical application. Hence, we prepared a novel drug delivery system—Baicalin liposome (BA-LP) in previous research—which can improve some physical properties of BA. Therefore, we aimed to explore the effect of BA-LP on ALI mice induced by LPS. In pharmacokinetics study, the values of t 1 / 2 and AUC0- t in the BA-LP group were significantly higher than that of the BA group in normal mice, indicating that BA-LP could prolong the duration time in vivo of BA. The BA-LP group also showed a higher concentration in lung tissues than the BA group. Pharmacodynamics studies showed that BA-LP had a better effect than the BA group at the same dosage on reducing the W/D ratio, alleviating the lung injury score, and decreasing the proinflammatory factors (TNF-α, IL-1β) and total proteins in bronchoalveolar lavage fluids (BALF). In addition, the therapeutic effects of BA-LP showed a dose-dependent manner. Western blot analysis indicated that the anti-inflammatory action of BA could be attributed to the inhibition of the TLR4-NFκBp65 and JNK-ERK signaling pathways. These results suggest that BA-LP could be a valuable therapeutic candidate in the treatment of ALI.

scholarly journals Anti-Inflammatory Effect of Anti-TNF-α SiRNA Cationic Phosphorus Dendrimer Nanocomplexes Administered Intranasally in a Murine Acute Lung Injury Model

2017 ◽  
Vol 18 (8) ◽  
pp. 2379-2388 ◽  
Author(s):  
Adam Bohr ◽  
Nicolas Tsapis ◽  
Ilaria Andreana ◽  
Anais Chamarat ◽  
Camilla Foged ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Injury Model ◽  
Tnf Α ◽  
Lung Injury Model ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Low-dose carbon monoxide treatment attenuates early pulmonary neutrophil recruitment after acid aspiration

2008 ◽  
Vol 294 (4) ◽  
pp. L644-L653 ◽  
Author(s):  
Jean A. Nemzek ◽  
Christopher Fry ◽  
Omorodola Abatan
Keyword(s):  
Carbon Monoxide ◽  
Lung Injury ◽  
Low Dose ◽  
Acidic Solution ◽  
Pulmonary Inflammation ◽  
Neutrophil Recruitment ◽  
Acute Lung Inflammation ◽  
Vascular Integrity ◽  
Acid Aspiration ◽  
Blood Neutrophils

Exogenous carbon monoxide (CO) has anti-inflammatory and cytoprotective properties that show promise in the treatment of numerous pulmonary diseases. However, the effectiveness of CO in acute pulmonary injury associated with direct lung insult has not been shown conclusively. The purpose of this study was to determine if exogenous CO would modulate the pulmonary inflammation and lung injury that develops after acid aspiration. Groups of mice were given intratracheal (IT) injections of either saline or an acidic solution. After the IT injection, some of the mice in each group were allowed to spontaneously inhale CO (500 ppm). Mice exposed to CO for 6 h after IT acid had a significant decrease in bronchoalveolar lavage (BAL) fluid neutrophil counts and in histological evidence of lung injury. These results could not be explained by changes in BAL fluid chemokine levels or altered CXCR2 expression. The reduced neutrophil recruitment was associated with a decrease in the percentage of peripheral blood neutrophils expressing CD11b protein. However, within 24 h, the BAL neutrophil counts increased and were not different from animals without CO exposure. In addition, indices of vascular integrity were not different between animals with acid aspiration regardless of CO exposure at the later time point. These results showed that CO can modulate the early development of acute lung inflammation in this model of acid aspiration. Although these effects were eventually overwhelmed, the results suggest that CO may have efficacy during the initial treatment of aspiration lung injury.

scholarly journals Regional pulmonary inflammation in an endotoxemic ovine acute lung injury model

2012 ◽  
Vol 183 (2) ◽  
pp. 149-158 ◽  
Author(s):  
A. Fernandez-Bustamante ◽  
R.B. Easley ◽  
M. Fuld ◽  
D. Mulreany ◽  
D. Chon ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Pulmonary Inflammation ◽  
Injury Model ◽  
Lung Injury Model
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