scholarly journals Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1258
Author(s):  
N. Arias-Ramos ◽  
L. E. Ibarra ◽  
M. Serrano-Torres ◽  
B. Yagüe ◽  
M. D. Caverzán ◽  
...  

Conjugated polymer nanoparticles (CPNs) have emerged as advanced polymeric nanoplatforms in biomedical applications by virtue of extraordinary properties including high fluorescence brightness, large absorption coefficients of one and two-photons, and excellent photostability and colloidal stability in water and physiological medium. In addition, low cytotoxicity, easy functionalization, and the ability to modify CPN photochemical properties by the incorporation of dopants, convert them into excellent theranostic agents with multifunctionality for imaging and treatment. In this work, CPNs were designed and synthesized by incorporating a metal oxide magnetic core (Fe3O4 and NiFe2O4 nanoparticles, 5 nm) into their matrix during the nanoprecipitation method. This modification allowed the in vivo monitoring of nanoparticles in animal models using magnetic resonance imaging (MRI) and intravital fluorescence, techniques widely used for intracranial tumors evaluation. The modified CPNs were assessed in vivo in glioblastoma (GBM) bearing mice, both heterotopic and orthotopic developed models. Biodistribution studies were performed with MRI acquisitions and fluorescence images up to 24 h after the i.v. nanoparticles administration. The resulting IONP-doped CPNs were biocompatible in GBM tumor cells in vitro with an excellent cell incorporation depending on nanoparticle concentration exposure. IONP-doped CPNs were detected in tumor and excretory organs of the heterotopic GBM model after i.v. and i.t. injection. However, in the orthotopic GBM model, the size of the nanoparticles is probably hindering a higher effect on intratumorally T2-weighted images (T2WI) signals and T2 values. The photodynamic therapy (PDT)—cytotoxicity of CPNs was not either affected by the IONPs incorporation into the nanoparticles.

RSC Advances ◽  
2016 ◽  
Vol 6 (115) ◽  
pp. 114580-114586 ◽  
Author(s):  
Dongliang Yang ◽  
Shuwei Zhang ◽  
Yanling Hu ◽  
Jia Chen ◽  
Biqing Bao ◽  
...  

Novel AIE-active conjugated polymer nanoparticles with bright fluorescence emission and excellent photostability have been prepared for imaging in cells and zebrafish.


Author(s):  
Panagiota Koralli ◽  
Spyridon Tsikalakis ◽  
Maria Goulielmaki ◽  
Stella Arelaki ◽  
Janina Müller ◽  
...  

Conjugated polymer nanoparticles (CPNs) have emerged as a new promising class of cancer theranostic agents due to their desirable optical features, such as high absorption coefficient and photoluminescence quantum yields,...


2021 ◽  
Author(s):  
Dorota Lubanska ◽  
Sami Alrashed ◽  
Gage T. Mason ◽  
Fatima Nadeem ◽  
Mitchell DiPasquale ◽  
...  

Glioblastoma is one of the most aggressive types of cancer with median survival of only 15 months. Successful therapy is hampered by the existence of treatment resistant populations of stem-like tumour initiating cells (TICs) and poor blood-brain barrier drug penetration. Therapies capable of effectively targeting the TIC population are in high demand. Here, we synthesize spherical diketopyrrolopyrrole (DPP)-based conjugated polymer nanoparticles (CPNs) with an average diameter of 109 nm. The CPN were designed to include fluorescein-conjugated hyaluronic acid (HA), a ligand for the CD44 receptor present on one population of TICs. We demonstrate blood-brain barrier permeability of this system and concentration and cell cycle phase-dependent selective uptake of HA-CPNs in CD44 positive GBM-patient derived cultures. Interestingly, we found that uptake alone decreases stemness, invasive properties and proliferation of the CD44-TIC population in zebrafish PDX models in vivo. This study is the first to show surface moiety-driven selectivity of conjugated polymer nanoparticles in targeting TIC populations in brain cancer.


Small ◽  
2013 ◽  
Vol 9 (18) ◽  
pp. 3092-3092 ◽  
Author(s):  
Dan Ding ◽  
Jie Liu ◽  
Guangxue Feng ◽  
Kai Li ◽  
Yong Hu ◽  
...  

2020 ◽  
Vol 21 (18) ◽  
pp. 6597
Author(s):  
Chao-Cheng Chen ◽  
Yang-Yi Chen ◽  
Yi-Hsuan Lo ◽  
Ming-Hsien Lin ◽  
Chih-Hsien Chang ◽  
...  

Malignant melanoma is the most harmful type of skin cancer and its incidence has increased in this past decade. Early diagnosis and treatment are urgently desired. In this study, we conjugated picolinamide/nicotinamide with the pharmacophore of 131I-MIP-1145 to develop 131I-iodofluoropicolinamide benzamide (131I-IFPABZA) and 131I-iodofluoronicotiamide benzamide (131I-IFNABZA) with acceptable radiochemical yield (40 ± 5%) and high radiochemical purity (>98%). We also presented their biological characteristics in melanoma-bearing mouse models. 131I-IFPABZA (Log P = 2.01) was more lipophilic than 131I-IFNABZA (Log P = 1.49). B16F10-bearing mice injected with 131I-IFNABZA exhibited higher tumor-to-muscle ratio (T/M) than those administered with 131I-IFPABZA in planar γ-imaging and biodistribution studies. However, the imaging of 131I-IFNABZA- and 131I-IFPABZA-injected mice only showed marginal tumor uptake in A375 amelanotic melanoma-bearing mice throughout the experiment period, indicating the high binding affinity of these two radiotracers to melanin. Comparing the radiation-absorbed dose of 131I-IFNABZA with the melanin-targeted agents reported in the literature, 131I-IFNABZA exerts lower doses to normal tissues on the basis of similar tumor dose. Based on the in vitro and in vivo studies, we clearly demonstrated the potential of using 131I-IFNABZA as a theranostic agent against melanoma.


2010 ◽  
Vol 46 (10) ◽  
pp. 1617 ◽  
Author(s):  
Sehoon Kim ◽  
Chang-Keun Lim ◽  
Jinhee Na ◽  
Yong-Deok Lee ◽  
Kwangmeyung Kim ◽  
...  

2013 ◽  
Vol 25 (32) ◽  
pp. 4504-4510 ◽  
Author(s):  
Eilaf Ahmed ◽  
Stephen W. Morton ◽  
Paula T. Hammond ◽  
Timothy M. Swager

Small ◽  
2013 ◽  
Vol 9 (18) ◽  
pp. 3093-3102 ◽  
Author(s):  
Dan Ding ◽  
Jie Liu ◽  
Guangxue Feng ◽  
Kai Li ◽  
Yong Hu ◽  
...  

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