scholarly journals Impact of Alkanediols on Stratum Corneum Lipids and Triamcinolone Acetonide Skin Penetration

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1451
Author(s):  
Melanie Sigg ◽  
Rolf Daniels
Keyword(s):  
Stratum Corneum ◽  
Triamcinolone Acetonide ◽  
Alkyl Chain ◽  
Skin Penetration ◽  
Alkyl Chain Length ◽  
Drug Penetration ◽  
Confocal Raman Spectroscopy ◽  
Enhancing Effect ◽  
Stratum Corneum Lipid ◽  
Stratum Corneum Lipids

Alkanediols are widely used as multifunctional ingredients in dermal formulations. In addition to their preservative effect, considering their possible impact on drug penetration is also essential for their use. In the present study, the influence of 2-methyl-2,4-pentanediol, 1,2-pentanediol, 1,2-hexanediol and 1,2-octanediol on the skin penetration of triamcinolone acetonide from four different semisolid formulations was investigated. Furthermore, confocal Raman spectroscopy measurements were performed to examine the influence of the alkanediols on stratum corneum lipid content and order. Alkanediols were found to increase the penetration of triamcinolone acetonide. However, the extent depends strongly on the formulation used. In certain formulations, 1,2-pentanediol showed the highest effect, while in others the penetration-enhancing effect increased with the alkyl chain length of the alkanediol used. None of the tested alkanediols extracted lipids from the stratum corneum nor reduced its thickness. Notwithstanding the above, the longer-chained alkanediols cause the lipids to be converted to a more disordered state, which favors drug penetration. This behavior could not be detected for the shorter-chained alkanediols. Therefore, their penetration-enhancing effect is supposed to be related to an interaction with the hydrophilic regions of the stratum corneum.

scholarly journals Stratum corneum lipid liposomes for investigating skin penetration enhancer effects

RSC Advances ◽  
2018 ◽  
Vol 8 (48) ◽  
pp. 27464-27469 ◽  
Author(s):  
Mónika Bakonyi ◽  
Attila Gácsi ◽  
Szilvia Berkó ◽  
Anita Kovács ◽  
Erzsébet Csányi
Keyword(s):  
Stratum Corneum ◽  
Skin Penetration ◽  
Penetration Enhancer ◽  
Stratum Corneum Lipid ◽  
Stratum Corneum Lipid Liposomes ◽  
Lipid Liposomes

The aim of this work was to investigate the applicability of stratum corneum lipid liposomes as in vitro skin models for studying skin penetration enhancer effect of Kolliphor RH40 and Transcutol.

scholarly journals High-Throughput Screening of Potential Skin Penetration-Enhancers Using Stratum Corneum Lipid Liposomes: Preliminary Evaluation for Different Concentrations of Ethanol

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Pajaree Sakdiset ◽  
Yuki Kitao ◽  
Hiroaki Todo ◽  
Kenji Sugibayashi
Keyword(s):  
Stratum Corneum ◽  
High Throughput ◽  
High Throughput Screening ◽  
Skin Permeation ◽  
Skin Penetration ◽  
Penetration Enhancers ◽  
Skin Impedance ◽  
Preliminary Evaluation ◽  
Stratum Corneum Lipid ◽  
Stratum Corneum Lipid Liposomes

In this study, we developed a technique for high-throughput screening (HTS) of skin penetration-enhancers using stratum corneum lipid liposomes (SCLLs). A fluorescent marker, sodium fluorescein (FL), entrapped in SCLLs was prepared to provide a preliminary evaluation of the effect of different concentrations of ethanol on the disruption effect of SCLLs, which is an alternative for skin penetration-enhancing effects. In addition, SCLLs containing a fluorescent probe (DPH, TMA-DPH, or ANS) were also prepared and utilized to investigate SCLL fluidity. The results using SCLL-based techniques were compared with conventional skin permeation and skin impedance test using hairless rat skin. The obtained correlations were validated between FL leakage, SCLL fluidity with various probes, or skin impedance and increases in the skin permeation enhancement ratio (ER) of caffeine as a model penetrant. As a result, FL leakage and SCLL fluidity using ANS were considered to be good indices for the skin penetration-enhancing effect, suggesting that the action of ethanol on the SC lipid and penetration-enhancing is mainly on the polar head group of intercellular lipids. In addition, this screening method using SCLL could be utilized as an alternative HTS technique for conventional animal tests. Simultaneously, the method was found to be time-saving and sensitive compared with a direct assay using human and animal skins.

scholarly journals Skin Diseases Associated with the Depletion of Stratum Corneum Lipids and Stratum Corneum Lipid Substitution Therapy

2015 ◽  
Vol 28 (1) ◽  
pp. 42-55 ◽  
Author(s):  
Fitsum F. Sahle ◽  
Tsige Gebre-Mariam ◽  
Bodo Dobner ◽  
Johannes Wohlrab ◽  
Reinhard H.H. Neubert
Keyword(s):  
Stratum Corneum ◽  
Skin Diseases ◽  
Substitution Therapy ◽  
Stratum Corneum Lipid ◽  
Stratum Corneum Lipids

scholarly journals Electron Microscopic Observations of Stratum Corneum Intercellular Lipids in Normal and Atopic Dogs

2001 ◽  
Vol 38 (6) ◽  
pp. 720-723 ◽  
Author(s):  
A. O. Inman ◽  
T. Olivry ◽  
S. M. Dunston ◽  
N. A. Monteiro-Riviere ◽  
H. Gatto
Keyword(s):  
Stratum Corneum ◽  
Transepidermal Water Loss ◽  
Dry Skin ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Stratum Corneum Lipid ◽  
Structural Differences ◽  
Stratum Corneum Lipids ◽  
Intercellular Lipids ◽  
Intercellular Lipid

The barrier function of mammalian skin is maintained by intercellular stratum corneum lipids. In human patients with atopic dermatitis, an abnormal lipid barrier results in dry skin and increased transepidermal water loss. At this time, it is not known if a defective lipid barrier is present in atopic dogs. Normal and atopic canine skin were postfixed in ruthenium tetroxide and studied using transmission electron microscopy to determine structural differences within stratum corneum lipids. Intercellular lipid lamellae were graded on a semiquantitative scale. The deposition of stratum corneum lipid lamellae in atopic canine skin appeared markedly heterogeneous compared with that seen in normal canine skin. When present, the lamellae often exhibited an abnormal structure. The continuity and thickness of the intercellular lipid lamellae were significantly less in nonlesional atopic than in normal canine skin. These preliminary observations suggest that the epidermal lipid barrier is defective in atopic canine skin. Additional studies are needed to further characterize the biochemical defect and to possibly correct it with nutritional and/or pharmacologic intervention.

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