scholarly journals Enzyme-Responsive Amphiphilic Peptide Nanoparticles for Biocompatible and Efficient Drug Delivery

Pharmaceutics ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 143
Author(s):  
Su Jeong Song ◽  
Joon Sig Choi
Keyword(s):  
Drug Delivery ◽  
Hela Cells ◽  
Nile Red ◽  
Cell Penetration ◽  
Nanoparticle System ◽  
Amphiphilic Peptide ◽  
Peptide Nanoparticles ◽  
Self Assembled ◽  
Nanoparticle Structure ◽  
Formulation Stability

Self-assembled peptide nanostructures recently have gained much attention as drug delivery systems. As biomolecules, peptides have enhanced biocompatibility and biodegradability compared to polymer-based carriers. We introduce a peptide nanoparticle system containing arginine, histidine, and an enzyme-responsive core of repeating GLFG oligopeptides. GLFG oligopeptides exhibit specific sensitivity towards the enzyme cathepsin B that helps effective controlled release of cargo molecules in the cytoplasm. Arginine can induce cell penetration, and histidine facilitates lysosomal escape by its buffering capacity. Herein, we propose an enzyme-responsive amphiphilic peptide delivery system (Arg-His-(Gly-Phe-Lue-Gly)3, RH-(GFLG)3). The self-assembled RH-(GFLG)3 globular nanoparticle structure exhibited a positive charge and formulation stability for 35 days. Nile Red-tagged RH-(GFLG)3 nanoparticles showed good cellular uptake compared to the non-enzyme-responsive control groups with d-form peptides (LD (LRH-D(GFLG)3), DL (DRH-L(GFLG)3), and DD (DRH-D(GFLG)3). The RH-(GFLG)3 nanoparticles showed negligible cytotoxicity in HeLa cells and human RBCs. To determine the drug delivery efficacy, we introduced the anticancer drug doxorubicin (Dox) in the RH-(GFLG)3 nanoparticle system. LL-Dox exhibited formulation stability, maintaining the physical properties of the nanostructure, as well as a robust anticancer effect in HeLa cells compared to DD-Dox. These results indicate that the enzyme-sensitive RH-(GFLG)3 peptide nanoparticles are promising candidates as drug delivery carriers for biomedical applications.

Self-assembled nanoparticles based on a cationic conjugated polymer/hyaluronan–cisplatin complex as a multifunctional platform for simultaneous tumor-targeting cell imaging and drug delivery

2017 ◽  
Vol 41 (12) ◽  
pp. 4998-5006 ◽  
Author(s):  
Yan-Qin Huang ◽  
Rui Zhang ◽  
Yong-Kang Zhao ◽  
Hao Chen ◽  
Rong-Cui Jiang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Conjugated Polymer ◽  
Tumor Targeting ◽  
Cell Imaging ◽  
Multifunctional Nanoparticle ◽  
Nanoparticle System ◽  
Self Assembled ◽  
Multifunctional Platform

A multifunctional nanoparticle system based on a cationic conjugated polymer/hyaluronan–cisplatin complex for tumor-targeting cell imaging and drug delivery.

Self-assembled peptide nanoparticles with endosome escaping permits for co-drug delivery

Talanta ◽  
2021 ◽  
Vol 221 ◽  
pp. 121572
Author(s):  
Yingshu Guo ◽  
Yinhua Hu ◽  
Xiaofei Zheng ◽  
Xiuping Cao ◽  
Qiong Li ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Peptide Nanoparticles ◽  
Self Assembled

Self-Assembled Nanocarriers Based on Amphiphilic Natural Polymers for Anti- Cancer Drug Delivery Applications

2018 ◽  
Vol 23 (35) ◽  
Author(s):  
Sally Sabra ◽  
Mona Abdelmoneem ◽  
Mahmoud Abdelwakil ◽  
Moustafa Taha Mabrouk ◽  
Doaa Anwar ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cancer Drug ◽  
Natural Polymers ◽  
Anti Cancer ◽  
Drug Delivery Applications ◽  
Self Assembled ◽  
Cancer Drug Delivery

Multifunctional self-assembled cationic peptide nanostructures efficiently carry plasmid DNA in vitro and exhibit antimicrobial activity with minimal toxicity

2014 ◽  
Vol 2 (30) ◽  
pp. 4848-4861 ◽  
Author(s):  
Santosh Yadav ◽  
Manohar Mahato ◽  
Rajiv Pathak ◽  
Diksha Jha ◽  
Bipul Kumar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Plasmid Dna ◽  
Cationic Peptide ◽  
Minimal Toxicity ◽  
Amphiphilic Peptide ◽  
Self Assembled ◽  
Multifunctional Properties

An amphiphilic peptide–aminoglycoside (Pep–Neo) conjugate has been synthesized, self-assembled into nanostructures and evaluated for its multifunctional properties.

Self-assembled drug delivery systems. Part 6: In vitro/in vivo studies of anticancer N-octadecanoyl gemcitabine nanoassemblies

2012 ◽  
Vol 430 (1-2) ◽  
pp. 276-281 ◽  
Author(s):  
Yiguang Jin ◽  
Yanju Lian ◽  
Lina Du ◽  
Shuangmiao Wang ◽  
Chang Su ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
In Vivo Studies ◽  
Self Assembled

Thermo-responsive self-assembled polymeric micelles for drug delivery in vitro

2000 ◽  
Vol 205 (1-2) ◽  
pp. 165-172 ◽  
Author(s):  
In-Sook Kim ◽  
Young-Il Jeong ◽  
Chong-Su Cho ◽  
Sung-Ho Kim
Keyword(s):  
Drug Delivery ◽  
Polymeric Micelles ◽  
Self Assembled

Novel self-assembled pH-responsive biomimetic nanocarriers for drug delivery

2016 ◽  
Vol 64 ◽  
pp. 346-353 ◽  
Author(s):  
Minming Wu ◽  
Zhaoyu Cao ◽  
Yunfei Zhao ◽  
Rong Zeng ◽  
Mei Tu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Ph Responsive ◽  
Self Assembled

scholarly journals A Self-Assembling Amphiphilic Peptide Dendrimer-Based Drug Delivery System for Cancer Therapy

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1092
Author(s):  
Dandan Zhu ◽  
Huanle Zhang ◽  
Yuanzheng Huang ◽  
Baoping Lian ◽  
Chi Ma ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Drug Resistance ◽  
Cancer Therapy ◽  
Self Assembly ◽  
Cancer Drug ◽  
Acquired Drug Resistance ◽  
Self Assembling ◽  
Amphiphilic Peptide ◽  
Peptide Dendrimer

Despite being a mainstay of clinical cancer treatment, chemotherapy is limited by its severe side effects and inherent or acquired drug resistance. Nanotechnology-based drug-delivery systems are widely expected to bring new hope for cancer therapy. These systems exploit the ability of nanomaterials to accumulate and deliver anticancer drugs at the tumor site via the enhanced permeability and retention effect. Here, we established a novel drug-delivery nanosystem based on amphiphilic peptide dendrimers (AmPDs) composed of a hydrophobic alkyl chain and a hydrophilic polylysine dendron with different generations (AmPD KK2 and AmPD KK2K4). These AmPDs assembled into nanoassemblies for efficient encapsulation of the anti-cancer drug doxorubicin (DOX). The AmPDs/DOX nanoformulations improved the intracellular uptake and accumulation of DOX in drug-resistant breast cancer cells and increased permeation in 3D multicellular tumor spheroids in comparison with free DOX. Thus, they exerted effective anticancer activity while circumventing drug resistance in 2D and 3D breast cancer models. Interestingly, AmPD KK2 bearing a smaller peptide dendron encapsulated DOX to form more stable nanoparticles than AmPD KK2K4 bearing a larger peptide dendron, resulting in better cellular uptake, penetration, and anti-proliferative activity. This may be because AmPD KK2 maintains a better balance between hydrophobicity and hydrophilicity to achieve optimal self-assembly, thereby facilitating more stable drug encapsulation and efficient drug release. Together, our study provides a promising perspective on the design of the safe and efficient cancer drug-delivery nanosystems based on the self-assembling amphiphilic peptide dendrimer.

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