The Effect of a Host on the Primary Metabolic Profiling of Cuscuta Campestris’ Main Organs, Haustoria, Stem and Flower

Cuscuta campestris (dodder) is a stem holoparasitic plant without leaves or roots that parasitizes various types of host plants and causes damage to certain crops worldwide. This study aimed at gaining more knowledge about the effect of the hosts on the parasite’s levels of primary metabolites. To this end, metabolic profiling analyses were performed on the parasite’s three main organs, haustoria, stem and flowers, which developed on three hosts, Heliotropium hirsutissimum, Polygonum equisetiforme and Amaranthus viridis. The results showed significant differences in the metabolic profiles of C. campestris that developed on the different hosts, suggesting that the parasites rely highly on the host’s metabolites. However, changes in the metabolites’ contents between the organs that developed on the same host suggest that the parasite can also self-regulate its metabolites. Flowers, for example, have significantly higher levels of most of the amino acids and sugar acids, while haustoria and stem have higher levels of several sugars and polyols. Determination of total soluble proteins and phenolic compounds showed that the same pattern is detected in the organs unrelated to the hosts. This study contributes to our knowledge about the metabolic behavior of this parasite.

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DETERMINATION OF AMINO ACIDS AND SUGARS CONTENT IN ANTENNARIA DIOICA GAERTN

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019v11i5.33909 ◽

2019 ◽

pp. 39-43 ◽

Cited By ~ 2

Author(s):

LIUDMYLA SLOBODIANIUK ◽

LILIIA BUDNIAK ◽

SVITLANA MARCHYSHYN ◽

ROXOLANA BASARABA

Keyword(s):

Amino Acids ◽

Free Amino ◽

Hplc Method ◽

Biologically Active Substances ◽

Biologically Active ◽

New Drugs ◽

Raw Material ◽

Primary Metabolites ◽

Active Substances

Objective: The purpose of our study was to determine sugars and amino acids content of Antennaria dioica Gaertn. herb. In continuation of the investigation of biologically active substances from Antennaria dioica Gaertn., it advisable to study the qualitative composition and quantitative contents of sugars and amino acids from the herb of this plant. Methods: The herb of Antennaria dioica Gaertn. was analyzed for the content of sugars by GC/MS. The amino acids were identified and quantified by HPLC method. Results: The results of GC/MS analysis showed that in Antennaria dioica Gaertn. herb D-glucose had the highest content (7.16±0.09) mg/g, followed by D-fructose (5.27±0.06) mg/g and D-saccharose (6.72±0.08) mg/g. In the raw material a large amount of monosaccharides derivative–Myo-inositol was revealed, a content of which was (2.12±0.06) mg/g. We determined 17 bound and 16 free amino acids in the Antennaria dioica Gaertn. by HPLC method. Conclusion: The contents of primary metabolites provide opportunities for creating medicine and food supplements. The results show that Antennaria dioica Gaertn. is a rich source of these important biologically active substances. The resulting data will be used with the further purpose to produce new drugs of natural origin.

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Nitrogenous compounds, phenolic compounds and morphological aspects of leaves: comparison of deciduous and semideciduous arboreal legumes

Scientia Agricola ◽

10.1590/s0103-90162006000100007 ◽

2006 ◽

Vol 63 (1) ◽

pp. 40-45 ◽

Cited By ~ 3

Author(s):

Ana Lúcia da Silva Lima ◽

Fábio Zanella ◽

Marlene Aparecida Schiavinato ◽

Claudia Regina Baptista Haddad

Keyword(s):

Amino Acids ◽

Phenolic Compounds ◽

Natural Habitat ◽

Secondary Compounds ◽

Evergreen Species ◽

Nitrogenous Compounds ◽

Hymenaea Courbaril ◽

Morphological Aspects ◽

Protein Amino Acids

In general, perennial species contain higher concentrations of certain secondary compounds, such as phenolics, lower levels of nitrogenous compounds, and greater specific leaf mass (SLM) than deciduous species. The aim of this study was to verify whether the comparative data reported in the literature regarding deciduous and evergreen species, also applies to four selected species of a semideciduous tropical forest (a remnant of the Atlantic Forest). The four species chosen for this study, each with apparently different leaf life spans, were two semideciduous (Hymenaea courbaril L. var. stilbocarpa (Hayne) Lee et Lang., and Lonchocarpus guilleminianus (Tul.) Malme, and two deciduous (Enterolobium contortisiliquum (Vell.) Morong., and Peltophorum dubium (Spreng.) Taub.). Plants were grown in a greenhouse in plastic pots containing soil taken from their natural habitat. Mature leaves were harvested for determination of the SLM and contents of phenolic compounds, tannins, nitrate, protein, free amino acids, chlorophyll, and nitrogen. Data registered are in agreement with those of the literature. Leaves with the longest life span had the highest content of phenolics and the lowest levels of tannins, nitrate, protein, amino acids, chlorophyll and nitrogen, as well as presenting the greatest SLM.

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Determination of amino acids content of the Tagetes lucida Cav. by GC/MS

Pharmacia ◽

10.3897/pharmacia.68.e73325 ◽

2021 ◽

Vol 68 (4) ◽

pp. 859-867

Author(s):

Liudmyla Slobodianiuk ◽

Liliia Budniak ◽

Svitlana Marchyshyn ◽

Liliya Kostyshyn ◽

Maria Ezhned

Keyword(s):

Amino Acids ◽

Free Amino ◽

Raw Materials ◽

Food Reinforcement ◽

Raw Material ◽

Primary Metabolites ◽

Plant Raw Materials ◽

Prevention And Treatment ◽

Amino Acids Composition

Plant raw materials are widely used for the prevention and treatment providing of many diseases. The interest is the in-depth research of the flowers, leaves, and herb of Tagetes lucida. Therefore, the study aimed to determine the content of primary metabolites, namely amino acids in the raw materials of this plant. The amino acids composition and content in flowers, leaves, and herb were determined by the GC/MS method. The results of the study revealed that the raw material of Tagetes lucida contains more bound and less free amino acids. Free and bound L-proline, L-isoleucine were present in all the analyzed samples in the greatest amount (1.909 mg/g and 20.999 mg/g, 0.804 mg/g and 18.908 mg/g in the flowers; 2.721 mg/g and 18.973 mg/g, 3.459 mg/g and 28.518 mg/g in the leaves; 6.436 mg/g and 18.817 mg/g, 0.245 mg/g and 0.222 mg/g in the herb). Another free amino acid with a high content in flowers (1.321 mg/g) and herb (0.825 mg/g) of Tagetes lucida was L-aspartic acid. In addition, high content of L-phenylalanine in bound form was found in the leaves (11.843 mg/g) of the study plant. These amino acids to be considered distinguishing markers of the Tagetes lucida. This research contributes to already known information of Tagetes lucida use as herbal medicine, nutraceutical, and food reinforcement.

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