An R2R3-MYB Transcription Factor OsMYBAS1 Promotes Seed Germination under Different Sowing Depths in Transgenic Rice

MYB-type transcription factors play essential regulatory roles in seed germination and the response to seedling establishment stress. This study isolated a rice R2R3-MYB gene, OsMYBAS1, and functionally characterized its role in seed germination by generating transgenic rice plants with the overexpression and knockout of OsMYBAS1. Gene expression analysis suggested that OsMYBAS1 was highly expressed in brown rice and root, respectively. Subcellular localization analysis determined that OsMYBAS1 was localized in the nucleus. No significant differences in seed germination rate were observed among wild-type (WT) and transgenic rice plants at the 0-cm sowing depth. However, when sown at a depth of 4 cm, higher germination rates, root lengths and seedling heights were obtained in OsMYBAS1-overexpressing plants than in WT. Furthermore, the opposite results were recorded between the osmybas1 mutants and WT. Moreover, OsMYBAS1-overexpressing plants significantly enhanced superoxide dismutase (SOD) enzyme activity and suppressed the accumulation of malondialdehyde (MDA) content at the 4-cm sowing depth. These results indicate that the MYB transcription factor OsMYBAS1 may promote rice seed germination and subsequent seedling establishment under deep-sowing conditions. These findings can provide valuable insight into rice seed-quality breeding to facilitate the development of a dry, direct-seeding production system.

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OsNAC111, a Blast Disease–Responsive Transcription Factor in Rice, Positively Regulates the Expression of Defense-Related Genes

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-03-14-0065-r ◽

2014 ◽

Vol 27 (10) ◽

pp. 1027-1034 ◽

Cited By ~ 35

Author(s):

Naoki Yokotani ◽

Tomoko Tsuchida-Mayama ◽

Hiroaki Ichikawa ◽

Nobutaka Mitsuda ◽

Masaru Ohme-Takagi ◽

...

Keyword(s):

Transcription Factor ◽

Transgenic Rice ◽

Transcriptional Activation ◽

Rice Blast ◽

Rice Blast Fungus ◽

Blast Disease ◽

Pr Genes ◽

Rice Plants ◽

Blast Fungus ◽

Transgenic Rice Plants

Plants respond to pathogen attack by transcriptionally regulating defense-related genes via various types of transcription factors. We identified a transcription factor in rice, OsNAC111, belonging to the TERN subgroup of the NAC family that was transcriptionally upregulated after rice blast fungus (Magnaporthe oryzae) inoculation. OsNAC111 was localized in the nucleus of rice cells and had transcriptional activation activity in yeast and rice cells. Transgenic rice plants overexpressing OsNAC111 showed increased resistance to the rice blast fungus. In OsNAC111-overexpressing plants, the expression of several defense-related genes, including pathogenesis-related (PR) genes, was constitutively high compared with the control. These genes all showed blast disease-responsive expression in leaves. Among them, two chitinase genes and one β-1,3-glucanase gene showed reduced expression in transgenic rice plants in which OsNAC111 function was suppressed by a chimeric repressor (OsNAC111-SRDX). OsNAC111 activated transcription from the promoters of the chitinase and β-1,3-glucanase genes in rice cells. In addition, brown pigmentation at the infection sites, a defense response of rice cells to the blast fungus, was lowered in OsNAC111-SRDX plants at the early infection stage. These results indicate that OsNAC111 positively regulates the expression of a specific set of PR genes in the disease response and contributes to disease resistance.

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The NAC-type transcription factor GmNAC20 improves cold, salinity tolerance, and lateral root formation in transgenic rice plants

Functional & Integrative Genomics ◽

10.1007/s10142-021-00790-z ◽

2021 ◽

Author(s):

Rajesh Yarra ◽

Wei Wei

Keyword(s):

Transcription Factor ◽

Transgenic Rice ◽

Salinity Tolerance ◽

Lateral Root ◽

Root Formation ◽

Lateral Root Formation ◽

Rice Plants ◽

Transgenic Rice Plants

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EVALUATION OF THE POTENTIAL WEEDINESS AND BIOCHEMICAL PROPERTIES OF TRANSGENIC RICE WITH GLU-1DX5 GENE

KnE Life Sciences ◽

10.18502/kls.v2i1.200 ◽

2015 ◽

Vol 2 (1) ◽

pp. 495

Author(s):

Nono Carsono ◽

A. Quddus BM ◽

Diani Damayanti ◽

Gigih IP ◽

Murdaningsih HK ◽

...

Keyword(s):

Transgenic Rice ◽

Nutritive Value ◽

Biochemical Properties ◽

Rice Seed ◽

Rice Plants ◽

Transgenic Rice Plants ◽

Biochemical Evaluation ◽

Biochemical Testing ◽

Transgenic Rice Seed ◽

Screen House

<p>A Glu-1Dx5 allele, encoding for a high molecular weight glutenin sub unit Dx5, has been experimentally proven to be a major determinant for dough elasticity and functionality of bread wheat. Considering its important role, this gene has been successfully transferred to rice cv Fatmawati using particle bombardment. Some promising lines derived from consecutive generations have been obtained, however early biosafety assessment for transgenic plant is required in order to scientifically evaluate the equality of transgenic rice lines to their counterpart.Two promising lines (T3-19 and T3-20) which are supposed to be homozygous were subjected to weediness and biochemical evaluation. The evaluation of invasiveness as a measure of weediness was carried out in biosafety containment of BB Biogen, Bogor by growing a mixture of transgenic and nontransgenic. Biochemical testing was evaluated for nitrogen, carbohydrate, protein, fat, vitamin, ash, minerals (Ca and Mg), and 17 amino acids. Weediness testing revealed that competitiveness of transgenic rice plants grown in screen house as well as biosafety containment was equal with those of non transgenic plants, suggesting that transgenic rice plants have not high potency to be invasive. Transgenic rice seed had equal nutritive value with those of counterpart. These results indicate that transgenic rice plants (seeds) are substantially equivalent to non transgenic original lines except for the presence of Glu-1Dx5 gene. </p><p>Keywords: Glutenin, Invasiveness, Nutrient composition, Transgenic rice.</p>

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Cloning of New Herbicide Resistant Gene in Soil Metagenomics and Generation of Transgenic Rice Plants

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2014.01190 ◽

2014 ◽

Vol 40 (7) ◽

pp. 1190

Author(s):

Yun-Peng WANG ◽

Jing-Yong MA ◽

Rui MA ◽

Jian MA ◽

Wen-Guo LIU

Keyword(s):

Transgenic Rice ◽

Herbicide Resistant ◽

Rice Plants ◽

Resistant Gene ◽

Transgenic Rice Plants ◽

Soil Metagenomics

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A single nucleotide polymorphism in an R2R3 MYB transcription factor gene triggers the male sterility in soybean ms6 (Ames1)

Theoretical and Applied Genetics ◽

10.1007/s00122-021-03920-0 ◽

2021 ◽

Author(s):

Junping Yu ◽

Guolong Zhao ◽

Wei Li ◽

Ying Zhang ◽

Peng Wang ◽

...

Keyword(s):

Transcription Factor ◽

Glycine Max ◽

Male Sterility ◽

Bulked Segregant Analysis ◽

Soybean Protein ◽

Anther Development ◽

Hybrid Breeding ◽

Myb Transcription Factor ◽

Male Sterile ◽

R2r3 Myb

Abstract Key message Identification and functional analysis of the male sterile gene MS6 in Glycine max. Abstract Soybean (Glycine max (L.) Merr.) is an important crop providing vegetable oil and protein. The male sterility-based hybrid breeding is a promising method for improving soybean yield to meet the globally growing demand. In this research, we identified a soybean genic male sterile locus, MS6, by combining the bulked segregant analysis sequencing method and the map-based cloning technology. MS6, highly expressed in anther, encodes an R2R3 MYB transcription factor (GmTDF1-1) that is homologous to Tapetal Development and Function 1, a key factor for anther development in Arabidopsis and rice. In male sterile ms6 (Ames1), the mutant allele contains a missense mutation, leading to the 76th leucine substituted by histidine in the DNA binding domain of GmTDF1-1. The expression of soybean MS6 under the control of the AtTDF1 promoter could rescue the male sterility of attdf1 but ms6 could not. Additionally, ms6 overexpression in wild-type Arabidopsis did not affect anther development. These results evidence that GmTDF1-1 is a functional TDF1 homolog and L76H disrupts its function. Notably, GmTDF1-1 shows 92% sequence identity with another soybean protein termed as GmTDF1-2, whose active expression also restored the fertility of attdf1. However, GmTDF1-2 is constitutively expressed at a very low level in soybean, and therefore, not able to compensate for the MS6 deficiency. Analysis of the TDF1-involved anther development regulatory pathway showed that expressions of the genes downstream of TDF1 are significantly suppressed in ms6, unveiling that GmTDF1-1 is a core transcription factor regulating soybean anther development.

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