The NAC-type transcription factor GmNAC20 improves cold, salinity tolerance, and lateral root formation in transgenic rice plants

Plants respond to pathogen attack by transcriptionally regulating defense-related genes via various types of transcription factors. We identified a transcription factor in rice, OsNAC111, belonging to the TERN subgroup of the NAC family that was transcriptionally upregulated after rice blast fungus (Magnaporthe oryzae) inoculation. OsNAC111 was localized in the nucleus of rice cells and had transcriptional activation activity in yeast and rice cells. Transgenic rice plants overexpressing OsNAC111 showed increased resistance to the rice blast fungus. In OsNAC111-overexpressing plants, the expression of several defense-related genes, including pathogenesis-related (PR) genes, was constitutively high compared with the control. These genes all showed blast disease-responsive expression in leaves. Among them, two chitinase genes and one β-1,3-glucanase gene showed reduced expression in transgenic rice plants in which OsNAC111 function was suppressed by a chimeric repressor (OsNAC111-SRDX). OsNAC111 activated transcription from the promoters of the chitinase and β-1,3-glucanase genes in rice cells. In addition, brown pigmentation at the infection sites, a defense response of rice cells to the blast fungus, was lowered in OsNAC111-SRDX plants at the early infection stage. These results indicate that OsNAC111 positively regulates the expression of a specific set of PR genes in the disease response and contributes to disease resistance.

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An R2R3-MYB Transcription Factor OsMYBAS1 Promotes Seed Germination under Different Sowing Depths in Transgenic Rice

Plants ◽

10.3390/plants11010139 ◽

2022 ◽

Vol 11 (1) ◽

pp. 139

Author(s):

Xiaomin Wang ◽

Rong Wu ◽

Tongshu Shen ◽

Zhenan Li ◽

Chengyong Li ◽

...

Keyword(s):

Transcription Factor ◽

Seed Germination ◽

Transgenic Rice ◽

Seedling Establishment ◽

Myb Transcription Factor ◽

Rice Seed ◽

Sowing Depth ◽

Rice Plants ◽

Transgenic Rice Plants ◽

R2r3 Myb

MYB-type transcription factors play essential regulatory roles in seed germination and the response to seedling establishment stress. This study isolated a rice R2R3-MYB gene, OsMYBAS1, and functionally characterized its role in seed germination by generating transgenic rice plants with the overexpression and knockout of OsMYBAS1. Gene expression analysis suggested that OsMYBAS1 was highly expressed in brown rice and root, respectively. Subcellular localization analysis determined that OsMYBAS1 was localized in the nucleus. No significant differences in seed germination rate were observed among wild-type (WT) and transgenic rice plants at the 0-cm sowing depth. However, when sown at a depth of 4 cm, higher germination rates, root lengths and seedling heights were obtained in OsMYBAS1-overexpressing plants than in WT. Furthermore, the opposite results were recorded between the osmybas1 mutants and WT. Moreover, OsMYBAS1-overexpressing plants significantly enhanced superoxide dismutase (SOD) enzyme activity and suppressed the accumulation of malondialdehyde (MDA) content at the 4-cm sowing depth. These results indicate that the MYB transcription factor OsMYBAS1 may promote rice seed germination and subsequent seedling establishment under deep-sowing conditions. These findings can provide valuable insight into rice seed-quality breeding to facilitate the development of a dry, direct-seeding production system.

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Cloning of New Herbicide Resistant Gene in Soil Metagenomics and Generation of Transgenic Rice Plants

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2014.01190 ◽

2014 ◽

Vol 40 (7) ◽

pp. 1190

Author(s):

Yun-Peng WANG ◽

Jing-Yong MA ◽

Rui MA ◽

Jian MA ◽

Wen-Guo LIU

Keyword(s):

Transgenic Rice ◽

Herbicide Resistant ◽

Rice Plants ◽

Resistant Gene ◽

Transgenic Rice Plants ◽

Soil Metagenomics

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A Novel Mechanism Underlying Multi-walled Carbon Nanotube-Triggered Tomato Lateral Root Formation: the Involvement of Nitric Oxide

Nanoscale Research Letters ◽

10.1186/s11671-020-3276-4 ◽

2020 ◽

Vol 15 (1) ◽

Cited By ~ 2

Author(s):

Zeyu Cao ◽

Heng Zhou ◽

Lingshuai Kong ◽

Longna Li ◽

Rong Wang ◽

...

Keyword(s):

Nitric Oxide ◽

Carbon Nanotube ◽

Lateral Root ◽

Root Formation ◽

Lateral Root Formation ◽

Multi Walled Carbon Nanotube

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Lateral root formation and nutrients: nitrogen in the spotlight

Plant Physiology ◽

10.1093/plphys/kiab145 ◽

2021 ◽

Author(s):

Pierre-Mathieu Pélissier ◽

Hans Motte ◽

Tom Beeckman

Keyword(s):

Signaling Pathways ◽

Root System ◽

Root Development ◽

Lateral Root ◽

Molecular Mechanisms ◽

Root Formation ◽

Lateral Roots ◽

Nutrient Use Efficiency ◽

Lateral Root Formation ◽

Lateral Root Development

Abstract Lateral roots are important to forage for nutrients due to their ability to increase the uptake area of a root system. Hence, it comes as no surprise that lateral root formation is affected by nutrients or nutrient starvation, and as such contributes to the root system plasticity. Understanding the molecular mechanisms regulating root adaptation dynamics towards nutrient availability is useful to optimize plant nutrient use efficiency. There is at present a profound, though still evolving, knowledge on lateral root pathways. Here, we aimed to review the intersection with nutrient signaling pathways to give an update on the regulation of lateral root development by nutrients, with a particular focus on nitrogen. Remarkably, it is for most nutrients not clear how lateral root formation is controlled. Only for nitrogen, one of the most dominant nutrients in the control of lateral root formation, the crosstalk with multiple key signals determining lateral root development is clearly shown. In this update, we first present a general overview of the current knowledge of how nutrients affect lateral root formation, followed by a deeper discussion on how nitrogen signaling pathways act on different lateral root-mediating mechanisms for which multiple recent studies yield insights.

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