scholarly journals Generation of Transgenic Self-Incompatible Arabidopsis thaliana Shows a Genus-Specific Preference for Self-Incompatibility Genes

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 570 ◽  
Author(s):  
Tong Zhang ◽  
Guilong Zhou ◽  
Daphne R. Goring ◽  
Xiaomei Liang ◽  
Stuart Macgregor ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Species ◽  
The Self ◽  
Self Incompatibility ◽  
Arabidopsis Halleri ◽  
Downstream Signaling ◽  
Pollen Rejection ◽  
Brassicaceae Species ◽  
Its Gene ◽  
Self Compatibility

Brassicaceae species employ both self-compatibility and self-incompatibility systems to regulate post-pollination events. Arabidopsis halleri is strictly self-incompatible, while the closely related Arabidopsis thaliana has transitioned to self-compatibility with the loss of functional S-locus genes during evolution. The downstream signaling protein, ARC1, is also required for the self-incompatibility response in some Arabidopsis and Brassica species, and its gene is deleted in the A. thaliana genome. In this study, we attempted to reconstitute the SCR-SRK-ARC1 signaling pathway to restore self-incompatibility in A. thaliana using genes from A. halleri and B. napus, respectively. Several of the transgenic A. thaliana lines expressing the A. halleri SCR13-SRK13-ARC1 transgenes displayed self-incompatibility, while all the transgenic A. thaliana lines expressing the B. napus SCR1-SRK1-ARC1 transgenes failed to show any self-pollen rejection. Furthermore, our results showed that the intensity of the self-incompatibility response in transgenic A. thaliana plants was not associated with the expression levels of the transgenes. Thus, this suggests that there are differences between the Arabidopsis and Brassica self-incompatibility signaling pathways, which perhaps points to the existence of other factors downstream of B. napus SRK that are absent in Arabidopsis species.

scholarly journals Autophagy is required for self-incompatible pollen rejection in two transgenic Arabidopsis thaliana accessions

2020 ◽  
Author(s):  
Stuart R. Macgregor ◽  
Hyun Kyung Lee ◽  
Hayley Nelles ◽  
Daniel C. Johnson ◽  
Tong Zhang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
The Self ◽  
Self Incompatibility ◽  
Arabidopsis Lyrata ◽  
Additional Layer ◽  
Cellular Events ◽  
Pollen Rejection ◽  
Incompatible Pollen ◽  
Transgenic Lines ◽  
Stigmatic Papillae

AbstractSuccessful reproduction in the Brassicaceae is mediated by a complex series of interactions between the pollen and the pistil, and some species have an additional layer of regulation with the self-incompatibility trait. While the initial activation of the self-incompatibility pathway by the pollen S-locus protein11/S-locus cysteine-rich peptide and the stigma S Receptor Kinase is well characterized, the downstream mechanisms causing self-pollen rejection are still not fully understood. In previous studies, we had detected the presence of autophagic bodies with self-incompatible pollinations in Arabidopsis lyrata and transgenic A. thaliana lines, but it was not known if autophagy was essential for self-pollen rejection. Here, we investigated the requirement of autophagy in this response by crossing mutations in the essential AUTOPHAGY7 (ATG7) gene into two different transgenic self-incompatible A. thaliana lines in the Col-0 and C24 accessions. By using these previously characterized transgenic lines that express A. lyrata and A. halleri self-incompatibility genes, we demonstrated that disrupting autophagy can weaken their self-incompatible responses in the stigma. When the atg7 mutation was present, an increased number of self-incompatible pollen were found to hydrate and form pollen tubes that successfully fertilized the self-incompatible pistils. Additionally, we confirmed the presence of GFP-ATG8a labelled autophagosomes in the stigmatic papillae following self-incompatible pollinations. Together, these findings support the requirement of autophagy in the self-incompatibility response and add to the growing understanding of the cellular events that take place in the stigma to reject self-pollen.One Sentence SummaryIn self-incompatible transgenic Arabidopsis thaliana lines, autophagy is an integral part of the cellular responses in the stigma to efficiently block fertilization by self-incompatible pollen.

scholarly journals Patterns of polymorphism at the self-incompatibility locus in 1,083 Arabidopsis thaliana genomes

2017 ◽  
Author(s):  
Takashi Tsuchimatsu ◽  
Pauline M. Goubet ◽  
Sophie Gallina ◽  
Anne-Catherine Holl ◽  
Isabelle Fobis-Loisy ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Glacial Refugia ◽  
The Self ◽  
Ice Age ◽  
Self Incompatibility ◽  
S Locus ◽  
Sequencing Data ◽  
Functional Studies ◽  
Large Deletions ◽  
Last Ice Age

AbstractAlthough the transition to selfing in the model plant Arabidopsis thaliana involved the loss of the self-incompatibility (SI) system, it clearly did not occur due to the fixation of a single inactivating mutation at the locus determining the specificities of SI (the S-locus). At least three groups of divergent haplotypes (haplogroups), corresponding to ancient functional S-alleles, have been maintained at this locus, and extensive functional studies have shown that all three carry distinct inactivating mutations. However, the historical process of loss of SI is not well understood, in particular its relation with the last glaciation. Here, we took advantage of recently published genomic re-sequencing data in 1,083 Arabidopsis thaliana accessions that we combined with BAC sequencing to obtain polymorphism information for the whole S-locus region at a species-wide scale. The accessions differed by several major rearrangements including large deletions and inter-haplogroup recombinations, forming a set of haplogroups that are widely distributed throughout the native range and largely overlap geographically. ‘Relict’ A. thaliana accessions that directly derive from glacial refugia are polymorphic at the S-locus, suggesting that the three haplogroups were already present when glacial refugia from the last Ice Age became isolated. Inter-haplogroup recombinant haplotypes were highly frequent, and detailed analysis of recombination breakpoints suggested multiple independent origins. These findings suggest that the complete loss of SI in A. thaliana involved independent self-compatible mutants that arose prior to the last Ice Age, and experienced further rearrangements during post-glacial colonization.

scholarly journals Patterns of Polymorphism at the Self-Incompatibility Locus in 1,083 Arabidopsis thaliana Genomes

2017 ◽  
Vol 34 (8) ◽  
pp. 1878-1889 ◽  
Author(s):  
Takashi Tsuchimatsu ◽  
Pauline M. Goubet ◽  
Sophie Gallina ◽  
Anne-Catherine Holl ◽  
Isabelle Fobis-Loisy ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
The Self ◽  
Self Incompatibility ◽  
Incompatibility Locus

Assessment of Mating System in Helianthus annuus and H. petiolaris (Asteraceae) Populations

Helia ◽  
2020 ◽  
Vol 43 (72) ◽  
pp. 15-32
Author(s):  
Agustina Gutierrez ◽  
Daiana Scaccia Baffigi ◽  
Monica Poverene
Keyword(s):  
Gene Flow ◽  
Helianthus Annuus ◽  
Genetic System ◽  
The Self ◽  
Self Incompatibility ◽  
S Alleles ◽  
Self Fertilization ◽  
Self Compatibility ◽  
Incompatibility Alleles ◽  
Crop Area

AbstractHelianthus annuus subsp. annuus and H. petiolaris are wild North American species that have been naturalized in central Argentina. They have a sporophytic self-incompatibility genetic system that prevent self-fertilization but the occurrence of self-compatible plants in Argentina was observed in both species and could in part explain their highly invasive ability. Their geographical distribution coincides with the major crop area. The domestic sunflower is self-compatible, can hybridize with both species and presents a considerable amount of gene flow. The aim of this study is to understand the self-incompatibility mechanism in both wild Helianthus species. Reciprocal crossing and seed production were used to identify self-compatible genotypes, the number and distribution of self-incompatibility alleles within populations and the type and extent of allelic interactions in the pollen and pistil. The behaviour of S alleles within each population was explained by five functional S alleles and one non-functional allele in each species, differing in their presence and frequency within accessions. In both species, the allelic interactions were of dominance/recessiveness and codominance in pollen, whereas it was only codominance in the pistil. Inbreeding effects in wild materials appeared in the third generation of self-pollination, with lethal effects in most plants. The number of S alleles is low and they behave in a similar way of other Asteraceae species. The self-compatibility was addressed to non-functional S alleles introgressed in wild Helianthus plants through gene flow from self-compatible sunflower.

scholarly journals Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2186
Author(s):  
Zhiquan Liu ◽  
Bing Li ◽  
Yong Yang ◽  
Changbin Gao ◽  
Bin Yi ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Locus ◽  
Recognition System ◽  
Inbred Lines ◽  
The Self ◽  
Self Incompatibility ◽  
S Locus ◽  
S Haplotype ◽  
Self Compatibility

Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus.

scholarly journals Self-incompatibility Expression in Self-compatible Almond Genotypes May Be Due to Inbreeding

2005 ◽  
Vol 130 (6) ◽  
pp. 865-869 ◽  
Author(s):  
José Manuel Alonso ◽  
Rafael Socias i Company
Keyword(s):  
Pollen Tube ◽  
Differential Expression ◽  
Pollen Tube Growth ◽  
Pollen Tubes ◽  
Lower Number ◽  
The Self ◽  
Lower Percentage ◽  
Self Incompatibility ◽  
Prunus Amygdalus ◽  
Self Compatibility

Pollen tube growth after selfing was studied in four almond (Prunus amygdalus Batsch) families derived from crosses between self-compatible `Tuono' and self-incompatible `Ferragnès' and `Ferralise' in both directions, in order to ascertain the phenotypic expressions of the different genotypes. A differential expression of self-compatibility was observed in the seedlings of the different families. The genetic self-compatible offspring of `Ferralise' showed a lower percentage of pistils with pollen tubes at the style base and a lower number of pollen tubes at the pistil base after self-pollination than those observed in the self-compatible offspring of `Ferragnès'. This low level of self-compatibility expression observed in some `Ferralise' seedlings may be due to the inbreeding present in `Ferralise'. As a consequence, caution must be taken in almond breeding to avoid the increase of inbreeding by the utilization of related parents and to diversify the sources of self-compatibility, at present mostly limited to `Tuono.'

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