scholarly journals Development of a Full-Length Infectious cDNA Clone of the Grapevine Berry Inner Necrosis Virus

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1340
Author(s):  
Xudong Fan ◽  
Zunping Zhang ◽  
Fang Ren ◽  
Guojun Hu ◽  
Chen Li ◽  
...  

Grapevine berry inner necrosis virus (GINV) belongs to the genus Trichovirus in the family Betaflexiviridae. The GINV isolate LN_BETA_RS was obtained from a “Beta” grapevine (Vitis riparia × Vitis labrusca) exhibiting chlorotic mottling and ring spot in Xingcheng, Liaoning Province, China. To verify the correlation between GINV and grapevine chlorotic mottling and ring spot disease, we constructed an infectious cDNA clone of GINV isolate LN_BETA_RS using the seamless assembly approach. Applied treatments of agroinfiltration infectious cDNA confirmed systemic GINV infection of the Nicotianaoccidentalis 37B by reverse transcription polymerase chain reaction (RT-PCR) and transmission electron microscopy, exhibiting chlorotic mottling symptoms on leaves. Infectious cDNA was also transmitted to new healthy N. occidentalis plants through rub-inoculation. Moreover, the cDNA clone was agroinfiltrated into “Beta” and “Thompson Seedless” grapevine plantlets, and the inoculated grapevines exhibited leaf chlorotic mottling and ringspot during the two years of observation. GINV-inoculated “Beta” grapevines had serious leaf chlorotic mottling and ringspot symptoms on the whole plant, while relatively few symptoms were observed on the leaves of agroinoculated “Thompson Seedless” grapevines in early spring and only weak ring spot gradually appeared later in the top young leaves. Our experiments fulfilled Koch’s postulates and revealed the causative role of GINV in grapevine chlorotic mottling and ring spot disease.

2016 ◽  
Vol 161 (7) ◽  
pp. 2025-2027 ◽  
Author(s):  
Xudong Fan ◽  
Ni Hong ◽  
Zunping Zhang ◽  
Zuokun Yang ◽  
Fang Ren ◽  
...  

Plant Disease ◽  
2017 ◽  
Vol 101 (1) ◽  
pp. 144-149 ◽  
Author(s):  
X. D. Fan ◽  
Z. P. Zhang ◽  
F. Ren ◽  
G. J. Hu ◽  
J. Zhou ◽  
...  

To investigate the prevalence and genetic diversity of Grapevine berry inner necrosis virus (GINV) in China, 195 grapevine samples from 15 Chinese provinces and regions were tested using reverse-transcription polymerase chain reaction. The samples included symptomatic and asymptomatic cultivars, with 35.9% (70 of 195) of samples testing positive for GINV. Seventeen samples had obvious ring spot symptoms, and 94.1% (16 of 17) tested positive for GINV, suggesting that GINV may be highly associated with the ring spot symptom. The genetic diversity of GINV isolates was analyzed based on the partial nucleotide and amino acid sequences of the coat protein (CP) and movement protein (MP) genes. Phylogenetic analyses of the MP and CP gene sequences divided the GINV isolates into three groups. The majority of the Chinese isolates were in groups 1 and 2, and only one Chinese isolate, along with a previously reported Japanese isolate, was in group 3. This is the first report on the genetic diversity of GINV isolates and their prevalence and distribution in China.


2000 ◽  
Vol 31 (1) ◽  
pp. 59-60
Author(s):  
P. J.G.M. Steverink ◽  
J. M.A. Pol ◽  
J.N.A. Bos-de Ruijter ◽  
J. J.M. Meulenberg

Author(s):  
Hongqing Zhu ◽  
Yin Liu ◽  
Yingyun Cai ◽  
Dongdong Yu ◽  
Yinghui Pu ◽  
...  

2005 ◽  
Vol 21 (4) ◽  
pp. 361-368 ◽  
Author(s):  
Ju-Yeon Yoon ◽  
Jin-Sung Hong ◽  
Min-Jea Kim ◽  
Ju-Hee Ha ◽  
Gug-Seon Choi ◽  
...  

2002 ◽  
Vol 76 (16) ◽  
pp. 8494-8503 ◽  
Author(s):  
Christiane Meyer ◽  
Martina von Freyburg ◽  
Knut Elbers ◽  
Gregor Meyers

ABSTRACT Cloned cDNA derived from the genome of the virulent type 2 bovine viral diarrhea virus (BVDV) strain NY'93/C was sequenced and served for establishment of the infectious cDNA clone pKANE40A. Virus recovered from pKANE40A exhibited growth characteristics similar to those of wild-type BVDV NY'93/C and proved to be clinically indistinguishable from the wild-type virus in animal experiments. A virus mutant in which the RNase residing in the viral glycoprotein Erns was inactivated, revealed an attenuated phenotype. The plasmid pKANE40A represents the first infectious cDNA clone established for a type 2 BVDV and offers a variety of new approaches to analyze the mechanisms of BVDV-induced disease in cattle.


2009 ◽  
Vol 142 (1-2) ◽  
pp. 204-207 ◽  
Author(s):  
Wu-yang Zhu ◽  
Shi-hong Fu ◽  
Jing-lin Wang ◽  
Ying He ◽  
Qing Tang ◽  
...  

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