scholarly journals A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor

Polymers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1023
Author(s):  
Lili Guo ◽  
Shuang Chao ◽  
Pei Huang ◽  
Xiukai Lv ◽  
Quanquan Song ◽  
...  
Keyword(s):  
Schiff Base ◽  
Quartz Crystal Microbalance ◽  
Concanavalin A ◽  
Quartz Crystal ◽  
Sensor Surface ◽  
Con A ◽  
Ulex Europaeus ◽  
Plant Lectins ◽  
Photochemical Method ◽  
Ulex Europaeus Agglutinin I

A universal photochemical method to prepare carbohydrate sensors based on perfluorophenylazide (PFPA) modified polydopamine (PDA) for the study of carbohydrate–lectin interactions by a quartz crystal microbalance (QCM) biosensor was developed. The PFPA was immobilized on PDA-coated gold sensors via Schiff base reactions. Upon light irradiation, the underivatized carbohydrates were inserted into the sensor surface, including mannose, galactose, fucose and N-acetylglucosamine (GlcNAc). Carbohydrate sensors were evaluated for the binding to a series of plant lectins. A kinetic study of the interactions between mannose and concanavalin A (Con A), fucose and Ulex europaeus agglutinin I (UEA-I) were performed. This method can eliminate the tedious modification of carbohydrates, improve the experimental efficiency, and reduce the experimental cost, which is of great significance for the development of QCM biosensors and the study of biomolecular interactions.

scholarly journals Fabrication of Carbohydrate Chips Based on Polydopamine for Real-Time Determination of Carbohydrate–Lectin Interactions by QCM Biosensor

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1275 ◽  
Author(s):  
Kun Shang ◽  
Siyu Song ◽  
Yaping Cheng ◽  
Lili Guo ◽  
Yuxin Pei ◽  
...  
Keyword(s):  
Real Time ◽  
Con A ◽  
Quartz Crystals ◽  
Ulex Europaeus ◽  
Novel Approach ◽  
Ulex Europaeus Agglutinin I ◽  
Flow Through ◽  
Time Determination ◽  
Kinetics Of

A novel approach for preparing carbohydrate chips based on polydopamine (PDA) surface to study carbohydrate–lectin interactions by quartz crystal microbalance (QCM) biosensor instrument has been developed. The amino-carbohydrates were immobilized on PDA-coated quartz crystals via Schiff base reaction and/or Michael addition reaction. The resulting carbohydrate-chips were applied to QCM biosensor instrument with flow-through system for real-time detection of lectin–carbohydrate interactions. A series of plant lectins, including wheat germ agglutinin (WGA), concanavalin A (Con A), Ulex europaeus agglutinin I (UEA-I), soybean agglutinin (SBA), and peanut agglutinin (PNA), were evaluated for the binding to different kinds of carbohydrate chips. Clearly, the results show that the predicted lectin selectively binds to the carbohydrates, which demonstrates the applicability of the approach. Furthermore, the kinetics of the interactions between Con A and mannose, WGA and N-Acetylglucosamine were studied, respectively. This study provides an efficient approach to preparing carbohydrate chips based on PDA for the lectin–carbohydrate interactions study.

Kinetic analyses for bindings of concanavalin A to dispersed and condensed mannose surfaces on a quartz crystal microbalance

2009 ◽  
Vol 395 (2) ◽  
pp. 211-216 ◽  
Author(s):  
Toshiaki Mori ◽  
Momoko Toyoda ◽  
Tatsuro Ohtsuka ◽  
Yoshio Okahata
Keyword(s):  
Quartz Crystal Microbalance ◽  
Concanavalin A ◽  
Quartz Crystal

scholarly journals Preferential association of glycoproteins to the euchromatin regions of cross-fractured nuclei is revealed by fracture-label.

1986 ◽  
Vol 102 (2) ◽  
pp. 576-586 ◽  
Author(s):  
F W Kan ◽  
P P da Silva
Keyword(s):  
Concanavalin A ◽  
Colloidal Gold ◽  
Freeze Fracture ◽  
Ultrastructural Localization ◽  
Automated Image Analysis ◽  
Cell Nuclei ◽  
Con A ◽  
Gold Particles ◽  
Ulex Europaeus ◽  
Original Label

We used fracture-label to establish ultrastructural localization of glycoproteins in cross-fractured nuclei of duodenal columnar and exocrine pancreatic cells. Mannose residues were detected in cell nuclei by labeling freeze-fractured tissues with concanavalin A-horseradish peroxidase X colloidal gold (Con A-HRP X CG) or direct concanavalin A X colloidal gold (Con A X CG); fucose residues were detected with Ulex Europaeus I X colloidal gold (UEA I X CG) markers. Areas of the three main intranuclear compartments (euchromatin, heterochromatin, and nucleolus) exposed by freeze-fracture were determined by automated image analysis. Colloidal gold particles bound to each nuclear subcompartment were counted and the results expressed in number of colloidal gold particles per square micrometer +/- SEM. Duodenal and pancreatic tissues fractured and labeled with Con A-HRP X CG complex or direct Con A X CG conjugates showed that the vast majority of Con A binding sites was confined to euchromatin regions with only sparse labeling of the heterochromatin and nucleolus. UEA I labeling of duodenal columnar cells showed that colloidal gold particles were almost exclusively confined to cross-fractured areas where euchromatin is exposed. Trypsinization of the fractured tissues before labeling with Con A and UEA I abolished 95-100% of the original label. Our results show that, within the nucleoplasm, mannose and fucose are residues of glycoproteins preferentially located within the regions of euchromatin.

Immobilization of carbohydrate clusters on a quartz crystal microbalance sensor surface

2013 ◽  
Vol 393 ◽  
pp. 257-263 ◽  
Author(s):  
Masayo Ogiso ◽  
Koji Matsuoka ◽  
Tomoko Okada ◽  
Tomoko Imai ◽  
Miki Itoh ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Sensor Surface ◽  
Quartz Crystal Microbalance Sensor

scholarly journals Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Sri Wahyuni ◽  
Srihadi Agungpriyono ◽  
I. Ketut Mudite Adnyane ◽  
Hamny Hamny ◽  
Muhammad Jalaluddin ◽  
...  
Keyword(s):  
Histochemical Study ◽  
Ricinus Communis ◽  
Testicular Tissue ◽  
Spermatogenic Cells ◽  
Peanut Agglutinin ◽  
Con A ◽  
Ulex Europaeus ◽  
Ricinus Communis Agglutinin ◽  
Ulex Europaeus Agglutinin I ◽  
Muntiacus Muntjak

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 µg/ml of concentration for PNA lectins and 15µg/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis. Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak

Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface

2015 ◽  
Vol 69 (1) ◽  
Author(s):  
Tomáš Juřík ◽  
Petr Skládal
Keyword(s):  
Hydrogen Peroxide ◽  
Horseradish Peroxidase ◽  
Modified Electrode ◽  
Quartz Crystal Microbalance ◽  
Electrode Surface ◽  
Quartz Crystal ◽  
Sensor Surface ◽  
Low Concentration ◽  
Enzyme Product ◽  
Insoluble Product

AbstractBiocatalysed precipitation of an insoluble product accumulated on the enzyme-modified electrode surface was applied as the amplification path for low concentration sensing of hydrogen peroxide and glucose. Sensitive electrochemical and quartz-crystal microbalance (QCM) biosensors based on biocatalytic precipitation were developed. A horseradish peroxidase (HRP) monolayer-modified electrode was used to sense H

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