scholarly journals Fabrication of Carbohydrate Chips Based on Polydopamine for Real-Time Determination of Carbohydrate–Lectin Interactions by QCM Biosensor

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1275 ◽  
Author(s):  
Kun Shang ◽  
Siyu Song ◽  
Yaping Cheng ◽  
Lili Guo ◽  
Yuxin Pei ◽  
...  

A novel approach for preparing carbohydrate chips based on polydopamine (PDA) surface to study carbohydrate–lectin interactions by quartz crystal microbalance (QCM) biosensor instrument has been developed. The amino-carbohydrates were immobilized on PDA-coated quartz crystals via Schiff base reaction and/or Michael addition reaction. The resulting carbohydrate-chips were applied to QCM biosensor instrument with flow-through system for real-time detection of lectin–carbohydrate interactions. A series of plant lectins, including wheat germ agglutinin (WGA), concanavalin A (Con A), Ulex europaeus agglutinin I (UEA-I), soybean agglutinin (SBA), and peanut agglutinin (PNA), were evaluated for the binding to different kinds of carbohydrate chips. Clearly, the results show that the predicted lectin selectively binds to the carbohydrates, which demonstrates the applicability of the approach. Furthermore, the kinetics of the interactions between Con A and mannose, WGA and N-Acetylglucosamine were studied, respectively. This study provides an efficient approach to preparing carbohydrate chips based on PDA for the lectin–carbohydrate interactions study.

2008 ◽  
Vol 99 (13) ◽  
pp. 5786-5792 ◽  
Author(s):  
Mohamed Kheireddine Aroua ◽  
S.P.P. Leong ◽  
L.Y. Teo ◽  
Chun Yang Yin ◽  
Wan Mohd Ashri Wan Daud

1997 ◽  
Vol 3 (3) ◽  
pp. 231-247 ◽  
Author(s):  
L. H. Catalani ◽  
A. M. Rabello ◽  
F. H. Florenzano ◽  
M. J. Politi ◽  
W. F. Reed

2009 ◽  
Vol 25 (3) ◽  
pp. 122-127 ◽  
Author(s):  
Dirk Ertel ◽  
Tobias Pflederer ◽  
Stephan Achenbach ◽  
Willi A. Kalender

2002 ◽  
Vol 81 (15) ◽  
pp. 2863-2865 ◽  
Author(s):  
S. Martini ◽  
A. A. Quivy ◽  
E. C. F. da Silva ◽  
J. R. Leite

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Shicai Xu ◽  
Jian Zhan ◽  
Baoyuan Man ◽  
Shouzhen Jiang ◽  
Weiwei Yue ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document