scholarly journals Assessment of the Release of Vascular Endothelial Growth Factor from 3D-Printed Poly-ε-Caprolactone/Hydroxyapatite/Calcium Sulfate Scaffold with Enhanced Osteogenic Capacity

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1455 ◽  
Author(s):  
Cheng-Yu Chen ◽  
Chien-Chang Chen ◽  
Chen-Ying Wang ◽  
Alvin Kai-Xing Lee ◽  
Chun-Liang Yeh ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Drug Release ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Calcium Sulfate ◽  
Potential Candidate ◽  
Vascular Endothelial ◽  
Fused Deposition ◽  
Endothelial Growth Factor

Vascular endothelial growth factor (VEGF) is one of the most crucial growth factors and an assistant for the adjustment of bone regeneration. In this study, a 3D scaffold is fabricated using the method of fused deposition modeling. Such a fabricated method allows us to fabricate scaffolds with consistent pore sizes, which could promote cellular ingrowth into scaffolds. Therefore, we drafted a plan to accelerate bone regeneration via VEGF released from the hydroxyapatite/calcium sulfate (HACS) scaffold. Herein, HACS will gradually degrade and provide a suitable environment for cell growth and differentiation. In addition, HACS scaffolds have higher mechanical properties and drug release compared with HA scaffolds. The drug release profile of the VEGF-loaded scaffolds showed that VEGF could be loaded and released in a stable manner. Furthermore, initial results showed that VEGF-loaded scaffolds could significantly enhance the proliferation of human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVEC). In addition, angiogenic- and osteogenic-related proteins were substantially increased in the HACS/VEGF group. Moreover, in vivo results revealed that HACS/VEGF improved the regeneration of the rabbit’s femur bone defect, and VEGF loading improved bone tissue regeneration and remineralization after implantation for 8 weeks. All these results strongly imply that the strategy of VEGF loading onto scaffolds could be a potential candidate for future bone tissue engineering.

scholarly journals Moscow State University of Medicine and Dentistry named after A.I. Evdokimov

2021 ◽  
Vol 19 (4) ◽  
pp. 343-349
Author(s):  
G. A. Volozhin ◽  
E. A. Bazikian ◽  
R. V. Deev ◽  
I. E. Bozo ◽  
E. A. Presnyakov
Keyword(s):  
Tissue Engineering ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Bone Tissue ◽  
Plasmid Dna ◽  
Octacalcium Phosphate ◽  
Vascular Endothelial ◽  
Growth Factor Gene ◽  
Tissue Engineering Construct ◽  
Endothelial Growth Factor

Relevance. The effect of a tissue engineering construct based on synthetic octacalcium phosphate activated with plasmid DNA with vascular endothelial growth factor gene on bone morphogenesis at the jaw defect sites of patients was studied. It is shown that the studied osteoplastic material stimulates osteosynthesis pathways already at early stages, and xenogenic hydroxyapatite, triggers osteogenesis processes with considerable delay and does not have time to form a full-fledged bone structure by 6 months.Aim. Evaluate the dynamics of reparative osteogenesis based on the results of histomorphometric diagnostics in patients with defects in the jaw bones of various configurations and lengths with an implanted bone matrix based on synthetic octacalcium phosphate activated with plasmid deoxyribonucleic acid with genes of vascular endothelial growth factor.Materials and methods. Histomorphological examination of bone tissue biopsy of jaws was carried out in 50 patients of both genders, who needed additional volume of bone tissue to install dental implantation. Patients were divided into 2 groups by type of grafted material. 6 months after the surgery, bone biopsies were taken from the bone sites at the stage of implant placement. Histomorphological patterns and histomycrophotograms were studied in bone biopcies.Results. In bone tissue biopsies of patients implanted with a tissue engineering construct based on synthetic octacalcium phosphate activated with plasmid DNA with vascular endothelial growth factor gene, it was revealed that after 6 months there was prevaluation of mature bone tissue (42,71%), with the proportion of differentiated plate bone tissue being < 90%.Conclusions. Histomorphometric analysis showed that in patients implanted with a tissue engineering construct based on synthetic octacalcium phosphate activated with plasmid DNA with vascular endothelial growth factor gene, after 6 months, early rearrangement of bone tissue into a mechanically dense and highly mineralized structure was detected. 

The Effect of Autogenous Bone Graft Mixed With Recombinant Human Vascular Endothelial Growth Factor on Bone Regeneration

2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Ufuk Tasdemir ◽  
Berrin İyilikçi ◽  
Melisa Cansu Aktürk ◽  
Özmen Ozmen ◽  
Alper Kizildağ ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

Nickel doped nanohydroxyapatite: vascular endothelial growth factor inducing biomaterial for bone tissue engineering

RSC Advances ◽  
2015 ◽  
Vol 5 (89) ◽  
pp. 72515-72528 ◽  
Author(s):  
B. Anu Priya ◽  
K. Senthilguru ◽  
T. Agarwal ◽  
S. N. Gautham Hari Narayana ◽  
S. Giri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Vascular Endothelial ◽  
Effective Option ◽  
Vegf Pathway ◽  
Endothelial Growth Factor

Biomaterial induced activation of vascular endothelial growth factor (VEGF) pathway for angiogenesis is now gaining recognition as an effective option for tissue engineering.

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