Analysis of the Cytotoxic Effects of Eryngium billardieri Delar. Extracts on MCF7 Cell Line

Background: Eryngium is genus flowering plants in the Umbelliferae family having pharmacological properties such as anti-inflammatory and anti-diabetic. Given the nature of melanoma and breast cancers in recent years and the fact that the anti-cancer properties of Eryngium billardieri on mentioned cell lines have not been studied, the present study conducted to explore these properties. Objective: The mechanisms of cytotoxicity and apoptosis of aerial parts of various extracts and fractions of E. billardieri on cancerous cells and normal cells were investigated. Methods: Samples were collected from natural habitats, dried and then extracted by Soxhlet apparatus with solvents of n-Hex, DCM and methanol, respectively. The cytotoxic effects of the extracts were investigated by MTT method on MCF7, B16 and HFF-2 classes for 24 and 48 hours. Flowcytometry, was also used to investigate the mechanism of cytotoxicity and confirming by Real-time PCR of p53 and Bax genes, which codes apoptosis regulator proteins. Meanwhile, volatile compounds of extracts were identified by GC-MS method. Results: The obtained data showed that the n-Hex extract of E. billardieri on B-16 and MCF7 cell lines and dichromethane extract on MCF7 cell line had the most significant cytotoxic effect compared to DMSO control (p value <0.001). Our finding showed that the mechanism of n-Hex extract with 80% and 100% vlc fractions on B16 induced apoptotic compared to HFF-2 control cells, moreover, n-Hex extract and 80% vlc fraction on MCF7 was apoptotic. The major compounds of n-Hex, DCM and 80% and 100% fractions of n-Hex extract obtained from GC-MS are non-terpenoid. Conclusion: Non-terpenoids compounds of E. billardieri can be the responsible for showing the most cytotoxic effects on MCF7 and B16 cell lines with apoptotic mechanism and n-Hex extract had the most significant inhibitory effect on cancerous cells compared to the HFF-2 control cells by the mechanism of apoptosis.

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Novel 1,8-Naphthyridine Derivatives: Design, Synthesis and in vitro screening of their cytotoxic activity against MCF7 cell line

Open Chemistry ◽

10.1515/chem-2019-0097 ◽

2019 ◽

Vol 17 (1) ◽

pp. 943-954

Author(s):

Abeer N. Al-romaizan ◽

Thoraya S. Jaber ◽

Nesreen S. Ahmed

Keyword(s):

Cell Line ◽

Human Breast Cancer ◽

Mcf7 Cell ◽

Cancer Cell Line ◽

Human Breast Cancer Cell ◽

The Other ◽

Human Breast ◽

Design Synthesis ◽

Mcf7 Cell Line

AbstractA series of new 2-phenyl-7-methyl-1,8-naphthyridine derivatives with variable substituents at C3 were synthesized for an in vitro evaluation of their anticancer activity against human breast cancer cell line (MCF7). On one hand, compounds 3f, 6f, 8c, and 10b showed IC50 values (6.53, 7.88, 7.89, 7.79 μM, respectively) compared to that of the mentioned drug staurosparine (IC50 = 4.51 μM). On the other hand, derivatives 10c, 8d, 4d, 10f and 8b displayed better activity than staurosporin with IC50 values (1.47, 1.62, 1.68, 2.30, 3.19 μM, respectively).

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Inference of hierarchical regulatory network of TCF7L2 binding sites in MCF7 cell line

International Journal of Computational Biology and Drug Design ◽

10.1504/ijcbdd.2016.074990 ◽

2016 ◽

Vol 9 (1/2) ◽

pp. 25 ◽

Cited By ~ 2

Author(s):

Yao Wang ◽

Rui Wang ◽

Victor X. Jin

Keyword(s):

Cell Line ◽

Binding Sites ◽

Regulatory Network ◽

Mcf7 Cell ◽

Mcf7 Cell Line

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The effects of TiO2 nanoparticles and doxorubicin complexes on the structure of DNA and inducing of apoptosis in MCF7 cell line

Clinical Biochemistry ◽

10.1016/j.clinbiochem.2011.08.173 ◽

2011 ◽

Vol 44 (13) ◽

pp. S77-S78

Author(s):

Hekmat Azadeh ◽

Saboury Ali Akbar

Keyword(s):

Cell Line ◽

Tio2 Nanoparticles ◽

Mcf7 Cell ◽

Mcf7 Cell Line

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Mixed ligand ruthenium arene complexes containing N-ferrocenyl amino acids: Biomolecular interactions and cytotoxicity against MCF7 cell line

Journal of Organometallic Chemistry ◽

10.1016/j.jorganchem.2017.01.020 ◽

2017 ◽

Vol 833 ◽

pp. 80-87 ◽

Cited By ~ 17

Author(s):

Pulipaka Ramadevi ◽

Rinky Singh ◽

Sarmita S. Jana ◽

Ranjitsinh Devkar ◽

Debjani Chakraborty

Keyword(s):

Amino Acids ◽

Cell Line ◽

Mcf7 Cell ◽

Mixed Ligand ◽

Biomolecular Interactions ◽

Arene Complexes ◽

Mcf7 Cell Line ◽

Ruthenium Arene

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Evaluation of anti-proliferative activity of simvastatin and atorvastatin on MCF7 cell line compared with doxorubicin using MTT test

Al Mustansiriyah Journal of Pharmaceutical Sciences ◽

10.32947/ajps.18.02.0386 ◽

2018 ◽

pp. 163-169

Keyword(s):

Cell Line ◽

Proliferative Activity ◽

Mcf7 Cell ◽

Mevalonate Pathway ◽

Pleiotropic Effect ◽

Receptor Protein ◽

Dependent Manner ◽

Proliferative Effect ◽

Mcf7 Cell Line ◽

Dose Dependent

Statins are effective to lower the cholesterol level and protect against cardiovascular disease. Recent studies showed that statins have pleiotropic effect and can be used to treat many types of diseases like neurodegenerative disorders, stroke, and cancer. Statins inhibit cell proliferation by suppression of mevalonate pathway leading to desregu-lation of cell signal transduction of some membrane receptor protein which is important for gene transcription. This study was done to evaluate the anti-proliferative activity of simvastatin and atorvastatin on MCF7 cell line alone and in combination with anathracycline chemotherapy drug (doxo-rubicin) using MTT assay. The results showed that simvastatin and atorvastatin had significant anti-proliferative effect on MCF7 cell line in dose-dependent manner with an IC50 10.10 μM and 12.3 μM respect-tively. Moreover, the combination of atorvastatin and simvastatin with (1 μM) doxorubicin had higher cytotoxic effect with an IC50 = 0.07 μM and 0.05μM respectively than doxoru-bicin alone IC50 = 1.9 μM. In conclusion, simvastatin and atorvastatin had anti-proliferative effect on MCF7 cell line and displayed significant synergism with doxorubicin which will help in enhancing efficacy of doxorubicin and decrease the adverse effect.

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Synthesis and antitumor activity of new isolated and fused heterobicyclic nitrogen systems containing 1,3,4-thiadiazole moiety derived from N1,N2-diaryl hydrazine compound

Letters in Applied NanoBioScience ◽

10.33263/lianbs91.935940 ◽

2020 ◽

Vol 9 (1) ◽

pp. 935-940

Keyword(s):

Antitumor Activity ◽

Cell Line ◽

Spectral Data ◽

13C Nmr ◽

Mass Spectra ◽

Mcf7 Cell ◽

Nitrogen Compounds ◽

Mcf7 Cell Line ◽

Ft Ir

New pyrazolyls and/ or 1,2,4-triazines bearing 1,3,4-thiadiazole moiety (3-10) have been synthesized via an interaction between 2-[5-(2-(4-chlorophenyl)hydrazineyl)-1,3,4-thiadiazol-2-yl]phenol and bi-oxygen/ chloro nitrogen compounds in different conditions. Former structures of the new systems have been established from their elemental and spectral data (FT-IR, 1H/ 13C NMR, Mass spectra). All the obtained compounds were evaluated against MCF7 cell line cytotoxicity and antitumor cells. Compounds 9>10>2>7>8>4>5>6 exhibited important action.

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Synthesis of artemisinic acid derived glycoconjugates and their anticancer studies

Organic & Biomolecular Chemistry ◽

10.1039/d0ob00216j ◽

2020 ◽

Vol 18 (12) ◽

pp. 2252-2263

Author(s):

Tharun K. Kotammagari ◽

Sayantan Paul ◽

Ganesh K. Barik ◽

Manas K. Santra ◽

Asish K. Bhattacharya

Keyword(s):

Cell Line ◽

Mcf7 Cell ◽

Click Reaction ◽

Anticancer Activities ◽

Artemisinic Acid ◽

Mcf7 Cell Line ◽

Line P

Twenty-four artemisinic acid glycoconjugate hybrids were synthesized using click reaction and evaluated for their anticancer activities against the MCF7 cell line.

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