scholarly journals Physical Modification of PLA for Increasing Its Durability (Polarizing Light Microscopy Study)

Proceedings ◽  
2019 ◽  
Vol 29 (1) ◽  
pp. 18
Author(s):  
Beatrice Girbaciu ◽  
Mariana Cristea ◽  
Daniela Ionita ◽  
Silvia Mathe ◽  
Doina Dimonie

The European Strategy for plastic materials in a circular economy underlines the importance of using renewable polymers for long-lasting applications. [...]

1986 ◽  
Vol 150 (3) ◽  
pp. 187-194 ◽  
Author(s):  
D. St. John Collier ◽  
James A. Pain ◽  
Derek G. D. Wight ◽  
Penny Lovat ◽  
Michael E. Bailey

1990 ◽  
Vol 38 (1) ◽  
pp. 79-85 ◽  
Author(s):  
J Lherminier ◽  
G Prensier ◽  
E Boudon-Padieu ◽  
A Caudwell

Flavescence dorée (FD), a grapevine yellows disease, is caused by a mycoplasma-like organism (MLO). A colloidal gold indirect immunolabeling technique identified MLO in salivary glands of a vector leafhopper, Euscelidius variegatus. After aldehyde fixation, tissue samples were prepared by cryoultramicrotomy or embedding in acrylic resins. Double fixation with aldehydes and osmium retroxide, followed by embedding in epon, was also performed. Thin or semi-thin serial sections were treated with polyclonal anti-FD-MLO rabbit antibodies, then with gold-conjugated anti-rabbit IgG. Labeling was revealed using the silver enhancement technique for light microscopy. MLO in frozen thin sections of glands were efficiently labeled. Optimal results were obtained with 4% paraformaldehyde-0.1% glutaraldehyde fixation and low-temperature embedding in LR White resin. Both scattered MLO and unusual dense forms of MLO were easily detected with the electron-dense gold probe. This method distinguished MLO from other membrane-limited bodies and provided a good tool for studying infection in large regions of FD-infected tissues by light microscopy.


2020 ◽  
Vol 51 (12) ◽  
pp. 5074-5083
Author(s):  
Martin Sserwadda ◽  
Nancy Nevejan ◽  
Ronald Ntanzi ◽  
Pieter Cornillie ◽  
Wim Van den Broeck ◽  
...  

1988 ◽  
Vol 22 (1) ◽  
pp. 76-82 ◽  
Author(s):  
H. S. Bal ◽  
N. G. Ghoshal

The histomorphology of the terminal bronchiolar region of the mouse, rat, hamster, guineapig, gerbil and rabbit was studied. Although some general structural similarities existed in the progressive intrapulmonary branching pattern of the airway tree between species, there were conflicting accounts in the literature about the presence of the respiratory bronchioles in common laboratory mammals. In our light microscopy study we failed to detect the existence of typical respiratory bronchioles with characteristic interruptions on their walls projecting into the alveoli. Frequently in these species the terminal bronchioles were short and abrupt, opening directly into several alveolar ductules.


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