scholarly journals Assessing Stream Thermal Heterogeneity and Cold-Water Patches from UAV-Based Imagery: A Matter of Classification Methods and Metrics

2021 ◽  
Vol 13 (7) ◽  
pp. 1379
Author(s):  
Johannes Kuhn ◽  
Roser Casas-Mulet ◽  
Joachim Pander ◽  
Juergen Geist

Understanding stream thermal heterogeneity patterns is crucial to assess and manage river resilience in light of climate change. The dual acquisition of high-resolution thermal infrared (TIR) and red–green–blue-band (RGB) imagery from unmanned aerial vehicles (UAVs) allows for the identification and characterization of thermally differentiated patches (e.g., cold-water patches—CWPs). However, a lack of harmonized CWP classification metrics (patch size and temperature thresholds) makes comparisons across studies almost impossible. Based on an existing dual UAV imagery dataset (River Ovens, Australia), we present a semi-automatic supervised approach to classify key riverscape habitats and associated thermal properties at a pixel-scale accuracy, based on spectral properties. We selected five morphologically representative reaches to (i) illustrate and test our combined classification and thermal heterogeneity assessment method, (ii) assess the changes in CWP numbers and distribution with different metric definitions, and (iii) model how climatic predictions will affect thermal habitat suitability and connectivity of a cold-adapted fish species. Our method was successfully tested, showing mean thermal differences between shaded and sun-exposed fluvial mesohabitats of up to 0.62 °C. CWP metric definitions substantially changed the number and distance between identified CWPs, and they were strongly dependent on reach morphology. Warmer scenarios illustrated a decrease in suitable fish habitats, but reach-scale morphological complexity helped sustain such habitats. Overall, this study demonstrates the importance of method and metric definitions to enable spatio-temporal comparisons between stream thermal heterogeneity studies.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Giulia Tedeschi ◽  
Lorenzo Scipioni ◽  
Maria Papanikolaou ◽  
Geoffrey W. Abbott ◽  
Michelle A. Digman

AbstractVoltage-gated potassium (Kv) channels are a family of membrane proteins that facilitate K+ ion diffusion across the plasma membrane, regulating both resting and action potentials. Kv channels comprise four pore-forming α subunits, each with a voltage sensing domain, and they are regulated by interaction with β subunits such as those belonging to the KCNE family. Here we conducted a comprehensive biophysical characterization of stoichiometry and protein diffusion across the plasma membrane of the epithelial KCNQ1-KCNE2 complex, combining total internal reflection fluorescence (TIRF) microscopy and a series of complementary Fluorescence Fluctuation Spectroscopy (FFS) techniques. Using this approach, we found that KCNQ1-KCNE2 has a predominant 4:4 stoichiometry, while non-bound KCNE2 subunits are mostly present as dimers in the plasma membrane. At the same time, we identified unique spatio-temporal diffusion modalities and nano-environment organization for each channel subunit. These findings improve our understanding of KCNQ1-KCNE2 channel function and suggest strategies for elucidating the subunit stoichiometry and forces directing localization and diffusion of ion channel complexes in general.


2021 ◽  
pp. 116927
Author(s):  
Bruna de Ramos ◽  
Melanie Vianna Alencar ◽  
Fábio Lameiro Rodrigues ◽  
Ana Luzia de Figueiredo Lacerda ◽  
Maíra Carneiro Proietti

2021 ◽  
Vol 58 (2) ◽  
pp. 281-299
Author(s):  
Somnath Bar ◽  
Bikash Ranjan Parida ◽  
Gareth Roberts ◽  
Arvind Chandra Pandey ◽  
Prasenjit Acharya ◽  
...  

Author(s):  
Ioannis T. Georgiou

A local damage at the tip of a composite propeller is diagnosed by properly comparing its impact-induced free coupled dynamics to that of a pristine wooden propeller of the same size and shape. This is accomplished by creating indirectly via collocated measurements distributed information for the coupled acceleration field of the propellers. The powerful data-driven modal expansion analysis delivered by the Proper Orthogonal Decomposition (POD) Transform reveals that ensembles of impact-induced collocated coupled experimental acceleration signals are underlined by a high level of spatio-temporal coherence. Thus they furnish a valuable spatio-temporal sample of coupled response induced by a point impulse. In view of this fact, a tri-axial sensor was placed on the propeller hub to collect collocated coupled acceleration signals induced via modal hammer nondestructive impacts and thus obtained a reduced order characterization of the coupled free dynamics. This experimental data-driven analysis reveals that the in-plane unit components of the POD modes for both propellers have similar shapes-nearly identical. For the damaged propeller this POD shape-difference is quite pronounced. The shapes of the POD modes are used to compute indices of difference reflecting directly damage. At the first POD energy level, the shape-difference indices of the damaged composite propeller are quite larger than those of the pristine wooden propeller.


2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Xin Wang ◽  
António Pinto-Duarte ◽  
M. Margarita Behrens ◽  
Xianjin Zhou ◽  
Terrence J. Sejnowski

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