scholarly journals Wavefront Shaping Concepts for Application in Optical Coherence Tomography—A Review

Sensors ◽  
2020 ◽  
Vol 20 (24) ◽  
pp. 7044
Author(s):  
Jonas Kanngiesser ◽  
Bernhard Roth
Keyword(s):  
Optical Coherence Tomography ◽  
Scattered Light ◽  
Three Dimensional ◽  
Medical Diagnostics ◽  
Optical Coherence ◽  
Scattering Media ◽  
Destructive Testing ◽  
Non Invasive ◽  
Wavefront Shaping ◽  
Micrometer Scale

Optical coherence tomography (OCT) enables three-dimensional imaging with resolution on the micrometer scale. The technique relies on the time-of-flight gated detection of light scattered from a sample and has received enormous interest in applications as versatile as non-destructive testing, metrology and non-invasive medical diagnostics. However, in strongly scattering media such as biological tissue, the penetration depth and imaging resolution are limited. Combining OCT imaging with wavefront shaping approaches significantly leverages the capabilities of the technique by controlling the scattered light field through manipulation of the field incident on the sample. This article reviews the main concepts developed so far in the field and discusses the latest results achieved with a focus on signal enhancement and imaging.

scholarly journals Non-invasive three-dimensional thickness analysis of oral epithelium based on optical coherence tomography—development and diagnostic performance

Heliyon ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. e06645
Author(s):  
Charlotte Theresa Trebing ◽  
Sinan Sen ◽  
Stefan Rues ◽  
Christopher Herpel ◽  
Maria Schöllhorn ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Diagnostic Performance ◽  
Three Dimensional ◽  
Oral Epithelium ◽  
Optical Coherence ◽  
Non Invasive

Intraluminal three-dimensional optical coherence tomography – a tool for imaging of the Eustachian tube?

2019 ◽  
Vol 133 (2) ◽  
pp. 87-94 ◽  
Author(s):  
R Schuon ◽  
B Mrevlje ◽  
B Vollmar ◽  
T Lenarz ◽  
G Paasche
Keyword(s):  
Optical Coherence Tomography ◽  
Eustachian Tube ◽  
Cross Sections ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Diagnostic Value ◽  
Optical Coherence ◽  
Experimental Conditions ◽  
Non Invasive ◽  
Tube Dysfunction

AbstractObjectivesThe cause of Eustachian tube dysfunction often remains unclear. Therefore, this study aimed to examine the feasibility and possible diagnostic use of optical coherence tomography in the Eustachian tube ex vivo.MethodsTwo female blackface sheep cadaver heads were examined bilaterally. Three conditions of the Eustachian tube were investigated: closed (resting position), actively opened and stented. The findings were compared (and correlated) with segmented histological cross-sections.ResultsIntraluminal placement of the Eustachian tube with the optical coherence tomography catheter was performed without difficulty. Regarding the limited infiltration depth of optical coherence tomography, tissues can be differentiated. The localisation of the stent was accurate as was the lumen.ConclusionThe application of optical coherence tomography in the Eustachian tube under these experimental conditions is considered to be a feasible, rapid and non-invasive diagnostic method, with possible diagnostic value for determining the luminal shape and superficial lining tissue of the Eustachian tube.

scholarly journals Non-Invasive Quantification of the Growth of Cancer Cell Colonies by a Portable Optical Coherence Tomography

Micromachines ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 35 ◽  
Author(s):  
Meng-Tsan Tsai ◽  
Bo-Huei Huang ◽  
Chun-Chih Yeh ◽  
Kin Fong Lei ◽  
Ngan-Ming Tsang
Keyword(s):  
Optical Coherence Tomography ◽  
Cancer Cell ◽  
Cellular Response ◽  
Tumor Development ◽  
Three Dimensional ◽  
Cancer Cell Line ◽  
Optical Coherence ◽  
Non Invasive

Investigation of tumor development is essential in cancer research. In the laboratory, living cell culture is a standard bio-technology for studying cellular response under tested conditions to predict in vivo cellular response. In particular, the colony formation assay has become a standard experiment for characterizing the tumor development in vitro. However, quantification of the growth of cell colonies under a microscope is difficult because they are suspended in a three-dimensional environment. Thus, optical coherence tomography (OCT) imaging was develop in this study to monitor the growth of cell colonies. Cancer cell line of Huh 7 was used and the cells were applied on a layer of agarose hydrogel, i.e., a non-adherent surface. Then, cell colonies were gradually formed on the surface. The OCT technique was used to scan the cell colonies every day to obtain quantitative data for describing their growth. The results revealed the average volume increased with time due to the formation of cell colonies day-by-day. Additionally, the distribution of cell colony volume was analyzed to show the detailed information of the growth of the cell colonies. In summary, the OCT provides a non-invasive quantification technique for monitoring the growth of the cell colonies. From the OCT images, objective and precise information is obtained for higher prediction of the in vivo tumor development.

scholarly journals Optical Coherence Tomography Angiography of Choroidal Neovascularisation

2016 ◽  
Vol 10 (01) ◽  
pp. 29 ◽  
Author(s):  
Lorenzo Iuliano ◽  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Three Dimensional ◽  
Choroidal Neovascularisation ◽  
Diagnostic Tools ◽  
Retinal Diseases ◽  
Optical Coherence ◽  
Promising Technique ◽  
Non Invasive ◽  
Dimensional Reconstruction

Optical coherence tomography angiography (OCTA) is a new promising technique able to provide a rapid, dyeless and non-invasive three-dimensional reconstruction of perfused vessels of retina and choroid. OCTA has been recently added to the diagnostic tools of retinal experts, and its role is still being explored in different retinal diseases. Chorodial neovascularisations (CNVs) are among the most significant disorders where OCTA is enhancing our diagnostic and classification skills, since it demonstrates an excellent capacity to identify and characterise each specific subtype of CNV.

scholarly journals First in vivo visualization of the human subarachnoid space and brain cortex via optical coherence tomography

2019 ◽  
Vol 12 ◽  
pp. 175628641984304 ◽  
Author(s):  
Karl Hartmann ◽  
Klaus-Peter Stein ◽  
Belal Neyazi ◽  
Ibrahim Erol Sandalcioglu
Keyword(s):  
Optical Coherence Tomography ◽  
Subarachnoid Space ◽  
Brain Cortex ◽  
Three Dimensional ◽  
Optical Coherence ◽  
Pia Mater ◽  
Anatomical Structures ◽  
Non Invasive ◽  
The Brain

The present work explores optical coherence tomography (OCT) as a suitable in vivo neuroimaging modality of the subarachnoid space (SAS). Patients ( n = 26) with frontolateral craniotomy were recruited. The temporal and frontal arachnoid mater and adjacent anatomical structures were scanned using microscope-integrated three-dimensional OCT, (iOCT). Analysis revealed a detailed depiction of the SAS (76.9%) with delineation of the internal microanatomical structures such as the arachnoid barrier cell membrane (ABCM; 96.2%), trabecular system (50.2%), internal blood vessels (96.2%), pia mater (26.9%) and the brain cortex (96.2%). Orthogonal distance measuring was possible. The SAS showed a mean depth of 570 µm frontotemporal. The ABCM showed a mean depth of 74 µm frontotemporal. These results indicate that OCT provides a dynamic, non-invasive tool for real-time imaging of the SAS and adjacent anatomical structures at micrometer spatial resolution. Further studies are necessary to evaluate the value of OCT during microsurgical procedures.

scholarly journals Double Interferometer Design for Independent Wavefront Manipulation in Spectral Domain Optical Coherence Tomography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jonas Kanngiesser ◽  
Maik Rahlves ◽  
Bernhard Roth
Keyword(s):  
Optical Coherence Tomography ◽  
Adaptive Optics ◽  
Three Dimensional ◽  
Beam Shaping ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Structured Illumination ◽  
Scattering Media ◽  
Light Modulator ◽  
Sd Oct

Abstract Spectral domain optical coherence tomography (SD-OCT) is a highly versatile method which allows for three dimensional optical imaging in scattering media. A number of recent publications demonstrated the technique to benefit from structured illumination and beam shaping approaches, e.g. to enhance the signal-to-noise ratio or the penetration depth with samples such as biological tissue. We present a compact and easy to implement design for independent wavefront manipulation and beam shaping at the reference and sample arm of the interferometric OCT device. The design requires a single spatial light modulator and can be integrated to existing free space SD-OCT systems by modifying the source arm only. We provide analytical and numerical discussion of the presented design as well as experimental data confirming the theoretical analysis. The system is highly versatile and lends itself for applications where independent phase or wavefront control is required. We demonstrate the system to be used for wavefront sensorless adaptive optics as well as for iterative optical wavefront shaping for OCT signal enhancement in strongly scattering media.

scholarly journals Three-dimensional segmentation and depth-encoded visualization of choroidal vasculature using swept-source optical coherence tomography

2021 ◽  
pp. 153537022110285
Author(s):  
Hao Zhou ◽  
Tommaso Bacci ◽  
K Bailey Freund ◽  
Ruikang K Wang
Keyword(s):  
Optical Coherence Tomography ◽  
Three Dimensional ◽  
Age Related Macular Degeneration ◽  
Optical Coherence ◽  
Full Thickness ◽  
Swept Source ◽  
Automated Method ◽  
Choroidal Imaging ◽  
Choroidal Vasculature ◽  
Choroidal Vessels

The choroid provides nutritional support for the retinal pigment epithelium and photoreceptors. Choroidal dysfunction plays a major role in several of the most important causes of vision loss including age-related macular degeneration, myopic degeneration, and pachychoroid diseases such as central serous chorioretinopathy and polypoidal choroidal vasculopathy. We describe an imaging technique using depth-resolved swept-source optical coherence tomography (SS-OCT) that provides full-thickness three-dimensional (3D) visualization of choroidal anatomy including topographical features of individual vessels. Enrolled subjects with different clinical manifestations within the pachychoroid disease spectrum underwent 15 mm × 9 mm volume scans centered on the fovea. A fully automated method segmented the choroidal vessels using their hyporeflective lumens. Binarized choroidal vessels were rendered in a 3D viewer as a vascular network within a choroidal slab. The network of choroidal vessels was color depth-encoded with a reference to the Bruch’s membrane segmentation. Topographical features of the choroidal vasculature were characterized and compared with choroidal imaging obtained with indocyanine green angiography (ICGA) from the same subject. The en face SS-OCT projections of the larger choroid vessels closely resembled to that obtained with ICGA, with the automated SS-OCT approach proving additional depth-encoded 3D information. In 16 eyes with pachychoroid disease, the SS-OCT approach added clinically relevant structural details, including choroidal thickness and vessel depth, which the ICGA studies could not provide. Our technique appears to advance the in vivo visualization of the full-thickness choroid, successfully reveals the topographical features of choroidal vasculature, and shows potential for further quantitative analysis when compared with other choroidal imaging techniques. This improved visualization of choroidal vasculature and its 3D structure should provide an insight into choroid-related disease mechanisms as well as their responses to treatment.

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