Sensitivity Validation of EWOD Devices for Diagnosis of Early Mortality Syndrome (EMS) in Shrimp Using Colorimetric LAMP–XO Technique

Electrowetting-on-dielectric (EWOD) is a microfluidic technology used for manipulating liquid droplets at microliter to nanoliter scale. EWOD has the ability to facilitate the accurate manipulation of liquid droplets, i.e., transporting, dispensing, splitting, and mixing. In this work, EWOD fabrication with suitable and affordable materials is proposed for creating EWOD lab-on-a-chip platforms. The EWOD platforms are applied for the diagnosis of early mortality syndrome (EMS) in shrimp by utilizing the colorimetric loop-mediated isothermal amplification method with pH-sensitive xylenol orange (LAMP–XO) diagnosis technique. The qualitative sensitivity is observed by comparing the limit of detection (LOD) while performing the LAMP–XO diagnosis test on the proposed lab-on-a-chip EWOD platform, alongside standard LAMP laboratory tests. The comparison results confirm the reliability of EMS diagnosis on the EWOD platform with qualitative sensitivity for detecting the EMS DNA plasmid concentration at 102 copies in a similar manner to the common LAMP diagnosis tests.

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Lower Limits of Detection for Single Biological Particles Using Impedance Spectroscopy

Design, Synthesis, and Applications ◽

10.1115/nano2005-87060 ◽

2005 ◽

Author(s):

Pahnit Seriburi ◽

Ashutosh Shastry ◽

Angelique Van’t Wout ◽

John Mittler ◽

Shih-Hui Chao ◽

...

Keyword(s):

Theoretical Model ◽

Impedance Spectroscopy ◽

Minimally Invasive ◽

Limit Of Detection ◽

Single Cells ◽

Lab On A Chip ◽

Minimally Invasive Approach ◽

Invasive Approach ◽

Membrane Bound ◽

Lower Limits

Single-cell impedance spectroscopy integrated with lab-on-a-chip systems provides a direct and minimally invasive approach for monitoring and characterizing properties of individual cells in real-time. Here we investigate the theoretical potential and limitations of this technique for analyzing single membrane-bound particles as small as 100 nm in diameter. Our theoretical model suggests a lower limit of detection for single cells on the order of a few microns.

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Identification and Characterization of Vibrio Bacteria Isolated from Shrimp Infected with Early Mortality Syndrome/acute Hepatopancreatic Necrosis Syndrome (EMS/AHPNS) in Viet Nam

Asian Fisheries Science ◽

10.33997/j.afs.2018.31.s1.021 ◽

2018 ◽

Vol 31S ◽

Author(s):

DANG THI HOANG OANH ◽

Keyword(s):

Early Mortality ◽

Viet Nam ◽

Early Mortality Syndrome ◽

Identification And Characterization

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Benefits of a Biofilm System and Low Ammonia Levels to Decrees the Early Mortality Syndrome in Shrimp Larvae (Litopenaeus vannamei) Infected with (Vibrio parahaemolyticus)

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2020/v9i330161 ◽

2020 ◽

pp. 31-40

Author(s):

Guillermo Galindo Reyes

Keyword(s):

Litopenaeus Vannamei ◽

Vibrio Parahaemolyticus ◽

Ammonia Concentration ◽

Early Mortality ◽

Shrimp Aquaculture ◽

Economic Losses ◽

Asian Countries ◽

Early Mortality Syndrome ◽

In Series ◽

System Water

The world shrimp aquaculture, has faced several problems, causing severe losses in shrimp hatcheries; between the most critical has been diseases such as early mortality syndrome (EMS) caused by (Vibrio parahaemolyticus). The EMS was initially detected in Asian countries; after, it was disseminated to Mexico and other countries. In Mexico, EMS caused severe economic losses during 2013-2016; and it has not yet been eradicated. Various causes for EMS have been reported; none is entirely accurate, but water quality is essential for successful shrimp aquaculture; therefore, the aim this work was evaluate the ammonia concentration effect on susceptibility to (EMS) on post-larvae (PL-15) shrimp (Litopenaeus vannamei) infected with (V. parahaemolyticus), using a biofilm system (water with, microalgae, dinoflagellates, protozoa and other planktonic microorganisms). So series of 5 flasks each one were arranged as following: Series S; 900 ml of filtered seawater (FSW) and 10 PL-15 shrimp per flask. Series SB; 840 ml of FSW, 60 ml of biofilm and 10 PL-15 shrimp. Series E; 900 ml of FSW, infected with 2 ml (V. parahaemolyticus) 106 CFU/ ml and 10 PL-15 shrimp. Series EN; fifteen flasks with 900 ml of FSW, 10 PL-15 shrimp, added with NH4Cl (0.535 mg/ml), to get 0.5, 1.0, 1.5, 2.0 and 2.5 mg/l final ammonia concentration in 5 sub-series of 3 flasks each one. During experiment, ammonia concentration and PL-15 shrimp mortality were evaluated in all flask. Ammonia concentration was higher in series EN than in series E; the same was observed in Series S respect to SB, but at lower values. At end of experiment, mortality in series EN was 90% Vs 60% in E. Similarly, mortality in series S was 10% Vs 0% in SB. This results confirm that the ammonia increases PL shrimp mortality, and biofilm system reduce ammonia and consequently PL-15 shrimp mortality.

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Droplet creator based on electrowetting-on-dielectric for lab on a chip

Science in China Series E ◽

10.1007/s11431-006-0248-6 ◽

2006 ◽

Vol 49 (2) ◽

pp. 248-256 ◽

Cited By ~ 3

Author(s):

Xuefeng Zeng ◽

Ruifeng Yue ◽

Jiangang Wu ◽

Huan Hu ◽

Liang Dong ◽

...

Keyword(s):

Lab On A Chip ◽

Electrowetting On Dielectric

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Low-Cost and Disposable Electrowetting-on-Dielectric Lab on a Chip With an Integrated Electrochemical Detector Fabricated by Screen-Printing Process

IEEE Sensors Journal ◽

10.1109/jsen.2019.2921789 ◽

2019 ◽

Vol 19 (19) ◽

pp. 8597-8604 ◽

Cited By ~ 2

Author(s):

Kessararat Ugsornrat ◽

Patiya Pasakon ◽

Chanpen Karuwan ◽

Chakrit Sriprachuabwong ◽

Thitima Maturos ◽

...

Keyword(s):

Screen Printing ◽

Low Cost ◽

Lab On A Chip ◽

Electrochemical Detector ◽

Printing Process ◽

Electrowetting On Dielectric

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Early Mortality Syndrome in Great Lakes Salmonines

Journal of Aquatic Animal Health ◽

10.1577/h04-012.1 ◽

2005 ◽

Vol 17 (1) ◽

pp. 1-3 ◽

Cited By ~ 27

Author(s):

Dale C. Honeyfield ◽

Scott B. Brown ◽

John D. Fitzsimons ◽

Donald E. Tillitt

Keyword(s):

Great Lakes ◽

Early Mortality ◽

Early Mortality Syndrome

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Flotation-Spectrophotometric Method for the Determination of Aluminium ion Using Xylenol Orange

E-Journal of Chemistry ◽

10.1155/2011/637913 ◽

2011 ◽

Vol 8 (4) ◽

pp. 1528-1535 ◽

Cited By ~ 1

Author(s):

F. Nekouei ◽

Sh. Nekouei

Keyword(s):

Standard Deviation ◽

Spectrophotometric Method ◽

Calibration Curve ◽

Limit Of Detection ◽

Xylenol Orange ◽

Molar Absorptivity ◽

Relative Standard ◽

Ion Associate ◽

Sensitive Method

A simple, fast, reproducible and sensitive method for the flotation- spectrophotometric determination of Al3+is reported. The apparent molar absorptivity (ε) of the ion associate was determined to be 8.35×104L mol-1cm-1. The calibration curve was linear in the concentration range of 1.0-50 ng mL-1of Al3+with a correlation coefficient of 0.9997. The limit of detection (LOD) was 0.621 ng mL. The relative standard deviation (RSD) at 10 and 30 ng mL-1of aluminium were 1.580 and 2.410% (n=7) respectively. The method was applied for measuring the amount of aluminium in water samples.

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Thermal conductance switching based on the actuation of liquid droplets through the electrowetting on dielectric (EWOD) phenomenon

Applied Thermal Engineering ◽

10.1016/j.applthermaleng.2015.11.098 ◽

2016 ◽

Vol 98 ◽

pp. 189-195 ◽

Cited By ~ 14

Author(s):

Gilhwan Cha ◽

Chang-Jin Kim ◽

Y. Sungtaek Ju

Keyword(s):

Thermal Conductance ◽

Liquid Droplets ◽

Electrowetting On Dielectric ◽

Conductance Switching

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Selection of Bacillus spp. isolates having enzyme producing ability and antagonistic to Vibrio parahaemolyticus causing the early mortality syndrome (EMS) on shrimp

Science and Technology Development Journal - Natural Sciences ◽

10.32508/stdjns.v1i6.612 ◽

2018 ◽

Vol 1 (6) ◽

pp. 23-31

Author(s):

Dung Thi Thanh Do ◽

Binh Thanh Le ◽

Duong Thi Dang Hoang ◽

Quang Dinh Vo ◽

Trang Thi Phuong Phan

Keyword(s):

Vibrio Parahaemolyticus ◽

Extracellular Enzymes ◽

Early Mortality ◽

Testing Methods ◽

Shrimp Ponds ◽

Early Mortality Syndrome ◽

Rdna Sequencing ◽

Potential Applications ◽

Bacillus Spp ◽

Selection Of

The aim of this study is to select some Bacillus isolates which are capable of yielding several beneficial enzymes and antagonism to Vibrio parahaemolyticus causing the EMS shrimp disease. In this study, we isolated and screened total of 54 Bacillus isolates from 30 mud, water and shrimp samples at shrimp ponds in Soc Trang province. Among these, 19 isolates were resistant against Vibrio parahaemolyticus strains causing the EMS shrimp disease via two testing methods. Three of them including NA2B13, NA10B2, NA8B1 isolates showed strongresistance and strong one to three kinds of extracellular enzymes to produce. Result of 16S rDNA sequencing and MALDI -TOF showed that NA2B13 and NA8B1 were Bacillus subtilis and NA10B2 was B. amyloliquefaciens. These two species were regarded safe and having potential applications in the production of biological products to prevent EMS shrimp disease.

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