scholarly journals AFM1 Detection in Milk by Fab’ Functionalized Si3N4 Asymmetric Mach–Zehnder Interferometric Biosensors

Toxins ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 409 ◽  
Author(s):  
Tatevik Chalyan ◽  
Cristina Potrich ◽  
Erik Schreuder ◽  
Floris Falke ◽  
Laura Pasquardini ◽  
...  
Keyword(s):  
Rate Constants ◽  
Maximum Level ◽  
Aflatoxin M1 ◽  
Kinetic Rate ◽  
Minimum Concentration ◽  
Milk Samples ◽  
Naturally Occurring ◽  
Kinetic Rate Constants ◽  
Concentrated Milk ◽  
Kinetics Of

Aflatoxins (AF) are naturally occurring mycotoxins, produced by many species of Aspergillus. Among aflatoxins, Aflatoxin M1 (AFM1) is one of the most frequent and dangerous for human health. The acceptable maximum level of AFM1 in milk according to EU regulation is 50 ppt, equivalent to 152 pM, and 25 ppt, equivalent to 76 pM, for adults and infants, respectively. Here, we study a photonic biosensor based on Si 3 N 4 asymmetric Mach–Zehnder Interferometers (aMZI) functionalized with Fab’ for AFM1 detection in milk samples (eluates). The minimum concentration of AFM1 detected by our aMZI sensors is 48 pM (16.8 pg/mL) in purified and concentrated milk samples. Moreover, the real-time detection of the ligand-analyte binding enables the study of the kinetics of the reaction. We measured the kinetic rate constants of the Fab’-AFM1 interaction.

The Role of Temperature in the Entanglement Kinetics of a Polymer Melt

1995 ◽  
Vol 62 (3) ◽  
pp. 794-801 ◽  
Author(s):  
R. S. Jeyaseelan ◽  
A. J. Giacomin
Keyword(s):  
Network Theory ◽  
Rate Constants ◽  
Polymer Melt ◽  
Large Amplitude Oscillatory Shear ◽  
Kinetic Rate ◽  
Kinetic Rate Constants ◽  
Different Temperatures ◽  
Transient Network ◽  
Kinetics Of

Large-amplitude oscillatory shear (LAOS) experiments were conducted at different temperatures on a molten low-density polyethylene standard, designated IUPAC LDPE X. Jeyaseelan et al. (1993) have successfully employed a simplification of transient network theory to describe the LAOS behavior of this polymer melt, at 150°C. The transient network is described by two kinetic rate constants, one for the formation of entanglements due to Brownian motion (k1), and another for the destruction of entanglements (k2) due to the imposed deformation. Upon comparison of the predictions of this transient network theory with the measured LAOS behavior of this polymer, we find that the kinetic rate constants k1 and k2 are invariant in the range of temperatures examined (150 to 190°C). The temperature dependence of departures from linear viscoelasticity is fully accounted for in the equilibrium entanglement kinetics.

Interfacial catalysis by phospholipase A2: determination of the interfacial kinetic rate constants

Biochemistry ◽  
1991 ◽  
Vol 30 (29) ◽  
pp. 7283-7297 ◽  
Author(s):  
Otto G. Berg ◽  
Bao Zhu Yu ◽  
Joe Rogers ◽  
Mahendra Kumar Jain
Keyword(s):  
Phospholipase A2 ◽  
Rate Constants ◽  
Kinetic Rate ◽  
Kinetic Rate Constants ◽  
Interfacial Catalysis

The matrix effects on kinetic rate constants of antibody–antigen interactions reflect solvent viscosity

1998 ◽  
Vol 217 (1-2) ◽  
pp. 51-60 ◽  
Author(s):  
Claire L. Morgan ◽  
David J. Newman ◽  
Jacky M. Burrin ◽  
Christopher P. Price
Keyword(s):  
Rate Constants ◽  
Matrix Effects ◽  
Kinetic Rate ◽  
Solvent Viscosity ◽  
Kinetic Rate Constants ◽  
The Matrix

Ammoniation of Whole Cottonseed at Atmospheric Pressure and Ambient Temperature to Reduce Aflatoxin M1 in Milk1

1982 ◽  
Vol 45 (4) ◽  
pp. 341-344 ◽  
Author(s):  
R. L. PRICE ◽  
O. G. LOUGH ◽  
W. H. BROWN
Keyword(s):  
Dairy Cattle ◽  
Atmospheric Pressure ◽  
Background Level ◽  
Maximum Level ◽  
Aflatoxin M1 ◽  
Milk Samples ◽  
Treatment Conditions ◽  
Bulk Tank ◽  
Whole Cottonseed ◽  
Lactating Dairy Cattle

Aflatoxin-contaminated cottonseed was treated with 1 1/2% ammonia and 10% water and packed into a 3 m in diameter by 30-m long polyethylene bag and held for 21 d. The ammoniated cottonseed was fed to lactating dairy cattle in two different trials. (a) Ammoniated cottonseed was added to a standard dairy ration for 90 cows at a level of 3.5 kg per cow per day for 19 d. Non-ammoniated cottonseed was then fed at the same level for 7 d. Daily analysis of the milk from the bulk tank was performed. No differences in aflatoxin M1 from the background level of 0.1 μg/L was noted until the 22nd day of feeding. The maximum aflatoxin M1 level was reached at 0.55 μg/L on days 25–27. Levels returned to 0.1 μg/L 4 d after resumption of the feeding of the standard dairy ration. (b) Two and two tenths kg per day of ammoniated or non-ammoniated seed were added to rations of each of 6 dairy cows for 7 d. Milk samples were taken two milkings before feeding of the cottonseed, at each milking during the feeding and for 7 d after cottonseed feeding ceased. Analysis of milk for aflatoxin M1 showed a maximum level of 1.8 μg/L from the group receiving the untreated cottonseed and a maximum of 0.18 from the group receiving the ammoniated meal. Milk from both groups was negative for aflatoxin M1 6 d after cottonseed feeding ceased. Ammoniation of whole cottonseed included in the ration of dairy cattle was effective in reducing the amount of aflatoxin M1 in their milk by approximately 90% but not in completely eliminating it at treatment conditions used.

The addition of methanol to Criegee intermediates

2019 ◽  
Vol 21 (32) ◽  
pp. 17760-17771 ◽  
Author(s):  
Gustavo J. R. Aroeira ◽  
Adam S. Abbott ◽  
Sarah N. Elliott ◽  
Justin M. Turney ◽  
Henry F. Schaefer
Keyword(s):  
Ab Initio ◽  
Rate Constants ◽  
Experimental Results ◽  
Kinetic Rate ◽  
Criegee Intermediates ◽  
Kinetic Rate Constants ◽  
High Level

High level ab initio methods are employed to study the addition of methanol to the simplest Criegee intermediates and its methylated analogue. Kinetic rate constants over a range of temperatures are computed and compared to experimental results.

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