Effects of the association between whole cottonseed and calcium salts of fatty acids on nutrient intake, feedlot performance, and carcass characteristics of Bos indicus animals offered a high-concentrate diet

This experiment evaluated the effects of feeding whole cottonseed (WC) and/or calcium salts of fatty acids (CSFA) on dry matter intake (DMI), performance, and carcass characteristics of Bos indicus animals receiving a high-concentrate diet during the finishing phase. On day 0, 96 Nellore bulls were blocked according to initial shrunk body weight (BW; 302 ± 26.7 kg) into group pens (4 animals/pen) and, within blocks, pens were randomly assigned to receive: 1) 15% of WC and 2% of CSFA [dry matter (DM) basis] of palm, cottonseed, and soybean oil (15WC; n = 6), 2) 10% of WC and 3% of CSFA (DM basis) of palm, cottonseed, and soybean oil (10WC; n = 6), 3) 5% of WC and 4% of CSFA (DM basis) of palm, cottonseed, and soybean oil (5WC; n = 6), and 4) 0% of WC and 5% of CSFA (DM basis) of palm, cottonseed, and soybean oil (0WC; n = 6). Diets were formulated to be isocaloric, isonitrogenous, and isolipidic. Experimental period lasted 108 days, whereas dry matter intake (DMI) was evaluated daily and blood samples and carcass measurements were obtained on days 0, 55, and 108 of the study. Upon slaughter on day 109, steaks were collected for determination of the chemical and fatty acid (FA) profile of the meat. No treatment effects (P ≥ 0.35) were observed on DMI, performance, average daily gain (ADG), carcass ultrasound measurements, and chemical variables of the steak. Nonetheless, including WC into the diets increased C12:0, C16:0, C16:1 trans-9, C17:0, C18:0, C18:1 cis-9, C18:2 cis-9,cis-12, C18:3 cis-9,cis-12,cis-15, saturated, and unsaturated FA intake (P < 0.01). Moreover, adding WC increased DMI fluctuation and feed efficiency (P = 0.03), but decreased marbling (P ≤ 0.03). A treatment × day interaction was observed (P < 0.01) for serum leptin concentration, as 10WC animals had greater leptin concentration on d 103 vs. other treatments (P < 0.01). Regarding steak FA profile, WC addition into the diet increased C18:2 cis-7,trans-9 and C18:3 cis-9,cis-12,cis-15 (P < 0.001), whereas saturated FA was quadratically affected (P = 0.02) and unsaturated FA was reduced for 15WC (P < 0.04). In summary, increasing levels of CSFA into isolipidic finishing diets containing WC did not negatively impact feedlot performance, but reduced feed efficiency and increased marbling scores of Bos indicus bulls, demonstrating its feasibility as a technology to improve carcass traits of low-marbling animals.

281 The Effects of Whole Cottonseed Supplementation on Performance, Breeding Soundness Exams, and Manganese Superoxide Dismutase Concentrations in the Blood and Semen of Beef Bulls

The objective of this research was to determine the effects of whole cottonseed containing gossypol on post-pubertal beef bulls. In a two-year study, 46 Angus and Red Angus bulls (body weight 457.0 ± 85.2 kg; 16 to 20-months of age) were stratified by BW and randomly assigned to 1 of 3 levels of whole cottonseed supplementation while receiving ad libitum roughage. The treatments included: DD (n = 14, control; 3.18 kg/d of dried distillers grain, W/D (n = 16; 1.59 kg/d of whole cottonseed and 1.59 kg/d of dried distillers grain), or WW (n = 16 3.18 kg/d of whole cottonseed). Diets were formulated to be isocaloric and isonitrogenous. In both years, bulls were weighed, administered a breeding soundness exam, and morphological characteristics of spermatozoa were measured on d 0, 28, and 60. In year 1, blood and semen samples were collected on d 0, 28, and 60 to measure manganese superoxide dismutase activity. There was no treatment effect for blood or semen manganese superoxide dismutase concentration (P > 0.255). There was a treatment effect (P < 0.02) for average daily gain as bulls fed WW gained less compared to those fed WD and DD (0.96, 1.23, and 1.39 kg/d, respectively). No treatment effect was observed for scrotal circumference (P > 0.50). There were no treatment × time interactions, treatment, or time main effects for percentages of normal spermatozoa, spermatozoa with coiled or folded tails, or proximal droplets (P > 0.12). There was a treatment effect on the amount of swollen midpiece or abnormal head as bull in the DD treatment had a greater (P < 0.02) percentage of other abnormalities compared to WW, but WD was not different (P > 0.16). Inclusion of whole cottonseed may affect average daily gain, but does not affect reproductive parameters of beef bulls.

317 Effect of the Whole Cottonseed with Pre-treatment on the Digestibility, Productivity, and Milk Fat Profile of Dairy Cattle

To improve the efficiency of whole cottonseed (WCS) on dairy cattle, crush and alkali treatment were combined (Table 1) in this study. The gastrointestinal tract digestibility of WCS were measured by in situ ruminal incubation and in vitro fermentation, respectively. Finally, animal feeding experiment was conducted to evaluate the effect of processed WCS on dairy cattle. A total of 30 Holstein dairy cows with similar physiological status were allocated to three groups (CON, Group I, and Group II). Ration for the CON group was a basic ration with no WCS, ration with about 8% non-processed WCS was for Group I (DM basis), while the Group II was provided by the ration added with 8% Crush-Alkali (4% mixed alkali solids with NaOH/CaO ratio at 1:1) treated WCS. Results of the in situ and in vitro experiments showed that, compared with non-treated WCS, Crushing-Alkali treatment groups significantly improved WCS ruminal effective degradation rate of dry matter (DM), crude protein (CP), ether extract (EE), and neutral detergent fiber (NDF) (P < 0.05). Furthermore, Crush-Alkali (4% mixed alkali solids with NaOH/CaO ratio at 1:1) was the most beneficial to improve WCS gastrointestinal degradation of DM, CP and NDF, as well as enhance WCS rumen-bypass of EE. With the lowest free gossypol content (P< 0.05). Results of animal feeding experiment showed that DMI, 4% fat corrected milk production (4% FCM), milk protein, milk fat, and content of short-chain saturated fatty acids in milk in Group II significantly increased (P < 0.05) compared with CON group. DMI, ω-6 polyunsaturated fatty acids (PUFA) in milk, and the ratio of ω-6 to ω-3 PUFA were significantly higher in Group II than that in Group I (P < 0.05). Additionally, free gossypol concentration in serum and milk, as well as parameters reflecting liver function were not notably different among the three groups (P > 0.05).

Health concerns of lambs fed cottonseed hulls combined with chitosan by examining the blood metabolic profile and histopathology of the kidney, liver, and rumen

This study analysed the effect of cottonseed hulls and chitosan in diets for lambs by determining the blood metabolite profile, and the histopathology of the kidney, liver, and rumen. Eighty non-castrated Santa Inês lambs, approximately 120 days of age and a mean initial body weight (b.w.) of 22.6 (standard deviation ± 2.2 kg) were assigned to a completely randomised design, with a 2 × 2 factorial arrangement. Two chitosan levels and two cottonseed forms were evaluated. The experimental diets were following: diet containing whole cottonseed hulls (WC) without the addition of chitosan; diet containing WC with 136 mg/kg b.w. chitosan added; diet containing ground cottonseed hulls (GC) without the addition of chitosan; diet containing GC with 136 mg/kg b.w. chitosan added. The blood metabolites and hepatic enzymes ALT, AST, GGT were not significantly influenced in the treatment groups, except for the serum cholesterol concentration which was lower (P < 0.05) when the chitosan was combined with the whole cottonseed hulls. There were histopathological alterations (P < 0.05) in the liver and kidney tissue and moderate changes in the rumen samples in the animals fed cottonseed without chitosan, however, when chitosan was added, the changes were less marked. The combination of chitosan with cottonseed hulls (ground or whole) can be supplied safely to feedlot finishing lambs without compromising their health.

Effect of high-concentrate diets with calcium lignosulfonate and cottonseed processing method on quantitative traits and non-carcass components of feedlot cull ewes

This study examined the effects of cottonseed processing form and the inclusion of calcium lignosulfonate in high-concentrate diets for feedlot cull ewes on carcass traits and non-carcass components. Thirty Santa Inês cull ewes with an average body weight of 44.2 ± 5.2 kg and an average age of 50 months were distributed into collective stalls in a completely randomized design. The treatments consisted of diets including whole cottonseed, crushed cottonseed, whole cottonseed treated with lignosulfonate (100 g/kg, as fed), crushed cottonseed treated with lignosulfonate (100 g/kg, as fed), and a control diet without cottonseed. The experimental diets did not influence (P>0.05) average daily weight gain (0.195 kg/day), slaughter weight (51.74 kg), or in vivo biometric and on-carcass measurements. There was no difference (P>0.05) in loin-eye area or subcutaneous fat thickness as evaluated in vivo by ultrasound. There was no diet effect on hot carcass weight and yield (24.8 kg and 47.8 %), cold carcass weight and yield (24.2 kg and 46.8 %), or chilling loss (2.1 %). Non-carcass components did not differ in response to the diets (P>0.05). Dietary inclusion of calcium lignosulfonate increases the proportions of udder and liver relative to empty body weight (P<0.05). Neither the cottonseed processing method nor the inclusion of calcium lignosulfonate in high-concentrate diets for cull ewes affects their performance, biometric or morphometric measurements, non-carcass components, or qualitative traits of their carcass.

In situ rumen degradation characteristics and bacterial colonization of whole cottonseed, cottonseed hull and cottonseed meal with different gossypol content

Regarding whole cottonseed (WCS), cottonseed meal (CSM), and cottonseed hull (CSH), in situ rumen incubation was applied to determine their nutrient and gossypol degradation characteristics and bacterial colonization profile in lactating Holstein cows. Nylon bags containing the cotton by-products were incubated for 0, 6, 12, 24, 36, 48 and 72 h in the rumen, respectively. The relationship between nutrient degradability and free gossypol (FG) content were examined, and the differences in the composition and inferred gene function of the colonized microbiota were studied. As a result, CSM presented highest effective degradability of dry matter, neutral detergent fibre and acid detergent fibre, but the highest effective degradability of crude protein was found in WCS. Free gossypol disappearance rate increased significantly in the first 6 h, and it reached approximately 94% at 72 h of incubation among all samples. The level of FG did not affect nutrient degradability of cotton by-products. Significant differences were noted in attached bacterial community structure among cotton by-products after 24 h rumen incubation. Among the most abundant taxa at genus level, a greater abundance of Cercis gigantea and Succiniclasticum was observed in WCS samples, whereas the CSH and CSM samples contained a greater proportion of Prevotella 1 and Rikenellaceae RC9 gut group. The redundancy analysis revealed that the level of neutral detergent fibre, ether extract, and FG in cotton by-products were significantly positive related with the composition of the attached bacteria. Collectively, our results revealed the dynamics of degradation characteristics, and the difference in the composition of bacterial colonization. These findings are of importance for the targeted improvement of cotton by-products nutrient use efficiency in ruminants and further understanding of the gossypol degradation mechanism in the rumen.

Evaluation of Whole Cottonseed Intake and in Situ digestibility in Beef Cattle

Whole cottonseed (WCS) is a byproduct of the cotton industry and can be used to supplement protein and energy to beef cattle. Given rapid industry changes in cotton variety use and their influence on WCS size, density, and quality characteristics, periodic evaluation of cotton byproducts is needed to determine appropriate feeding strategies. The objective of this study was to evaluate total WCS intake, gossypol intake, and digestibility of a widely-planted common variety of WCS in the southeastern U.S., Delta Pine 1646 (DP1646), compared with a low-gossypol variety, Ultra-Low Gossypol Cottonseed (ULGCS). Six Angus and Angus-cross calves (two steers and one bull per group) were used in a 28-day feeding trial in a crossover design. Each group was fed either DP1646 or ULGCS for a 7-d acclimation period followed by a 7-d measured intake period. Treatment groups were then switched to the other variety for a second acclimation and measured period. Intake was greater (P = 0.0011) for ULGCS than for DP1646, with an average intake of 2.20 ± 0.24 kg and 1.06 ± 0.24 kg, respectively. Free gossypol intake was not different (P = 0.9371) among treatments with an average daily intake of 13 ± 0.1 ppm and 2 ± 0.8 ppm for DP1646 and ULGCS, respectively. An in situ digestibility trial was conducted using two ruminally-cannulated steers to compare the 72-h digestibility of DP1646 and ULGCS. There were no differences (P = 0.5910) between treatments in total DM digestibility between WCS varieties or treatment by time interaction (P = 0.1270). When evaluating digestibility relative to time, differences were observed (P ≤ 0.0013) at 24, 48, and 72 h. These results indicate that WCS intake was greater for the ULGCS than DP1646, and significant digestibility of either variety was not observed until 24 h of digestion.

Cotton Byproduct Use in Southeastern Beef Cattle Diets: Quality, Intake, and Changes in Feed Characteristics

Cotton is grown on over 4.5 million hectares in the southeastern U.S. annually, and byproducts of cotton production are utilized as feedstuffs in beef systems. Cotton breeding efforts and variety recommendations for crop production represent potential changes in cotton seed size, density, and quality, which may influence whole cottonseed feed quality characteristics. Also, cotton breeding to reduce gossypol concentration without compromising yield may improve value in ruminant diets. A recent feed intake trial demonstrated greater consumption of ultra-low gossypol seed compared with seed derived from a more widely planted cotton variety (0.072% vs 0.53% free gossypol, respectively) in growing steers (1.9 and 1.1 kg/hd/d, respectively). Historically, cottonseed is limited in bull diets due to the associated of decreased fertility in males due to gossypol. However, a recent study found that feeding up to 3.2 kg/hd/d over a 60-d period to developing beef bulls did not affect sperm quality. Heat damage commonly occurs in cottonseed during storage after the ginning process, but the feed value of heat damaged cottonseed is unknown. A recent study indicated that in situ dry matter (52.2 vs 69.2%) and N (78.1 vs 91.6%) disappearance decreased for heat damaged compared to normal cottonseed, respectively. Cotton gin trash remains a prevalent byproduct with traditionally limited use. Some gins have equipment for baling gin trash in 180-to-270 kg modules, which allow ease of access and transport of gin trash waste for cow-calf producers. Similar to gin trash, grazing cotton crop residue after harvest can decrease the amount of hay fed during the winter. A recent 3-yr study indicated a 2.5 hd/ha stocking rate of mature, non-lactating cows over a 30-d period decreased hay consumption by 65 %. By understanding the feed characteristics and management needed to utilize cotton byproducts, cattle operations can decrease feed cost while maintaining animal performance.

Availability and Management of Cotton Byproducts in North Carolina: A State Survey

Whole cottonseed and cotton gin byproduct, also known as gin trash or cotton burrs, are known to be economical feed ingredients for beef cattle diets. There are several sources of information available to producers regarding the feeding of cotton byproducts; however, there is little information on the local supply chain and availability of these products. The objective was to survey cotton gins in North Carolina to determine how whole cottonseed and cotton gin byproduct are marketed. The 19-question survey was developed using Qualtrics survey software and emailed to all cotton gins in the state. Those who failed to respond to the weblink were contacted by phone. This resulted in a 73.5% response rate (n = 25 responses). Data were analyzed using the reporting feature of Qualtrics. On average, 7,716 metric tons of cottonseed and 1,523 metric tons of gin byproduct are produced per gin during a typical season. All respondents reported that the majority of their whole cottonseed is marketed out-of-state as livestock feed. All respondents reported that they either already sell or would consider selling cottonseed locally as cattle feed. In contrast, 78% of respondents reported that the majority of their gin byproduct stays in the state, with 40% being sold as cattle feed and the remaining 60% either being stockpiled and allowed to decompose on-site or used as compost. Of the respondents, 95% already sell gin byproduct locally as cattle feed or would consider doing so. The majority of respondents (92%) answered that they would like to be added to a directory to be distributed to cattle producers. This survey demonstrates that North Carolina cotton gins have cotton byproducts available and that they are interested in selling these locally to cattle producers.