scholarly journals Anti-Cancer Effect of Moroccan Cobra Naja haje Venom and Its Fractions against Hepatocellular Carcinoma in 3D Cell Culture

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 402
Author(s):  
Ayoub Lafnoune ◽  
Su-Yeon Lee ◽  
Jin-Yeong Heo ◽  
Imane Gourja ◽  
Bouchra Darkaoui ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Stellate Cell ◽  
Primary Liver Cancer ◽  
Gel Filtration ◽  
Cancer Cell Line ◽  
Public Health Problem ◽  
Anticancer Effect ◽  
Gel Filtration Chromatography ◽  
Naja Haje

Hepatocellular carcinoma (HCC) is the most common primary liver cancer in adults, the fifth most common malignancy worldwide and the third leading cause of cancer related death. An alternative to the surgical treatments and drugs, such as sorafenib, commonly used in medicine is necessary to overcome this public health problem. In this study, we determine the anticancer effect on HCC of Moroccan cobra Naja haje venom and its fraction obtained by gel filtration chromatography against Huh7.5 cancer cell line. Cells were grown together with WI38 human fibroblast cells, LX2 human hepatic stellate cell line, and human endothelial cells (HUVEC) in MCTS (multi-cellular tumor spheroids) models. The hepatotoxicity of venom and its fractions were also evaluated using the normal hepatocytes cell line (Fa2N-4 cells). Our results showed that an anti HCC activity of Moroccan cobra Naja haje venom and, more specifically, the F7 fraction of gel filtration chromatography exhibited the greatest anti-hepatocellular carcinoma effect by decreasing the size of MCTS. This effect is associated with a low toxicity against normal hepatocytes. These results strongly suggest that the F7 fraction of Moroccan cobra Naja haje venom obtained by gel filtration chromatography possesses the ability to inhibit cancer cells proliferation. More research is needed to identify the specific molecule(s) responsible for the anticancer effect and investigate their mechanism of action.

Anticancer effect of X-Ray triggered methotrexate conjugated albumin coated bismuth sulfide nanoparticles on SW480 colon cancer cell line

2020 ◽  
Vol 582 ◽  
pp. 119320
Author(s):  
Mohammad Hasan Faghfoori ◽  
Hamed Nosrati ◽  
Hamed Rezaeejam ◽  
Jalil Charmi ◽  
Saeed Kaboli ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Bismuth Sulfide ◽  
Anticancer Effect ◽  
X Ray

Black Phosphorus Nanoparticles Potentiate the Anticancer Effect of Oxaliplatin in Ovarian Cancer Cell Line

2017 ◽  
Vol 27 (36) ◽  
pp. 1701955 ◽  
Author(s):  
Michaela Fojtů ◽  
Xinyi Chia ◽  
Zdeněk Sofer ◽  
Michal Masařík ◽  
Martin Pumera
Keyword(s):  
Ovarian Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Ovarian Cancer Cell Line ◽  
Cancer Cell Line ◽  
Black Phosphorus ◽  
Anticancer Effect

scholarly journals ANTICANCER EFFECT OF SALVIA (MARAMIA) EXTRACT ON HEP-2 CANCER CELL LINE

2017 ◽  
Vol 63 (4) ◽  
pp. 3253-3261
Author(s):  
Ahmed Fahmi ◽  
Amr Elbolok ◽  
Naglaa Kamal
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Anticancer Effect

Synthesis, Characterization and Anti-hepatoma Activity of New Hederagenin Derivatives

2020 ◽  
Vol 20 (3) ◽  
pp. 252-257
Author(s):  
Xiao Liu ◽  
Lu Sun ◽  
Qing-Hua Liu ◽  
Bao-Quan Chen ◽  
Yu-Ming Liu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Normal Cell ◽  
Biological Significance ◽  
Cancer Cell Line ◽  
Human Hepatocellular Carcinoma ◽  
Hepg2 Cell Line ◽  
Normal Cell Line

Background: Based on the biological significance of hederagenin-type saponins found in our previous investigation, a series of new hederagenin derivatives were designed and synthesized. Methods : Their in vitro antiproliferative activities were evaluated against the HepG2 liver cancer cell line and normal cell line L929 by MTT assay. Results: The preliminary bioassay results demonstrated that all the tested compounds 1-7 showed potent anti-hepatoma activities, and some compounds exhibited better effects than 5-fluorouracil against human hepatocellular carcinoma HepG2 cell line. Furthermore, compound 5 showed a significant antihepatoma activity against HepG2 cells with an IC50 value of 1.88 µM. Besides, all of the tested compounds showed a low cytotoxic effect against the normal cell line L929. Conclusion: All the compounds 1-7 displayed superior selectivity against human hepatocellular carcinoma HepG2 cell line, and the results suggest that the structural modifications of C ring on the hederagenin backbone are vital for modulating anti-hepatoma activities.

scholarly journals Tumour calcitonin. Interaction with specific calcitonin receptors

1980 ◽  
Vol 190 (3) ◽  
pp. 545-550 ◽  
Author(s):  
J Ham ◽  
M L Ellison ◽  
J Lumsden
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Line ◽  
Medullary Thyroid Carcinoma ◽  
Gel Filtration ◽  
Bronchial Carcinoma ◽  
Binding Activity ◽  
Gel Filtration Chromatography ◽  
Medullary Thyroid ◽  
Molecular Weights ◽  
Receptor Binding Activity

The human epidermoid bronchial carcinoma (BEN) cell line has been shown to have specific membrane binding sites for calcitonin and to secrete high-molecular-weight forms (ranging from 40000 to 10000) of immunoreactive calcitonin. Synthetic salmon and human calcitonins and a thyroid extract of porcine calcitonin have been shown to displace 125I-labelled salmon calcitonin from the receptors in a dose-related fashion. The binding to these receptors of calcitonins derived from the BEN cell line and a medullary thyroid carcinoma with molecular weights ranging from 28000 to 3500 (both separated by gel-filtration chromatography) has been investigated. Neither major peaks of BEN-cell-line calcitonin showed receptor binding activity. Only one form of medullary thyroid carcinoma calcitonin, that which co-eluted with synthetic calcitonin monomer on gel-filtration chromatography, caused any significant displacement of labelled hormone from the receptors.

Erratum to Algerian Propolis Potentiates Doxorubicin Mediated Anticancer Effect Against Human Pancreatic PANC-1 Cancer Cell Line through Cell Cycle Arrest, Apoptosis Induction and P-Glycoprotein Inhibition

2019 ◽  
Vol 18 (9) ◽  
pp. 1364-1364
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Apoptosis Induction ◽  
Anticancer Effect ◽  
Cycle Arrest ◽  
P Glycoprotein ◽  
Glycoprotein Inhibition

Due to an oversight one of the author’s name was published wrong in the article entitled “Algerian Propolis Potentiates Doxorubicin Mediated Anticancer Effect Against Human Pancreatic PANC-1 Cancer Cell Line through Cell Cycle Arrest, Apoptosis Induction and P-Glycoprotein Inhibition” in “Anti-Cancer Agents in Medicinal Chemistry, 2018, Vol. 18, No. 3, pp. 375.” <p> The correct names of all authors are given below: <p> Rouibah H, Kebsa W, Lahouel M, Zihlif M, Ahram M, Aburmeleih B, Mustafa E, El-Ameer HJ.

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