scholarly journals Mutation in the Cadherin Gene Is a Key Factor for Pink Bollworm Resistance to Bt Cotton in China

Toxins ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 23
Author(s):  
Ling Wang ◽  
Dong Xu ◽  
Yunxin Huang ◽  
Huazhong Zhou ◽  
Weiguo Liu ◽  
...  

Transgenic crops producing Bacillus thuringiensis (Bt) toxins are widely planted for insect control, but their efficacy may decrease as insects evolve resistance. Understanding the genetic basis of insect resistance is essential for developing an integrated strategy of resistance management. To understand the genetic basis of resistance in pink bollworm (Pectinophora gossypiella) to Bt cotton in the Yangtze River Valley of China, we conducted an F2 screening for alleles associated with resistance to the Bt (Cry1Ac) protein for the first time. A total of 145 valid single-paired lines were screened, among which seven lines were found to carry resistance alleles. All field parents in those seven lines carried recessive resistance alleles at the cadherin locus, including three known alleles, r1, r13 and r15, and two novel alleles, r19 and r20. The overall frequency of resistance alleles in 145 lines was 0.0241 (95% CI: 0.0106–0.0512). These results demonstrated that resistance was rare and that recessive mutation in the cadherin gene was the primary mechanism of pink bollworm resistance to Bt cotton in the Yangtze River Valley of China, which will provide a scientific basis for implementing targeted resistance management statics of pink bollworm in this region.

2008 ◽  
Vol 27 (9) ◽  
pp. 1269-1276 ◽  
Author(s):  
Naiyin Xu ◽  
Michel Fok ◽  
Lixin Bai ◽  
Zhiguo Zhou

Water ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3294
Author(s):  
Chentao He ◽  
Jiangfeng Wei ◽  
Yuanyuan Song ◽  
Jing-Jia Luo

The middle and lower reaches of the Yangtze River valley (YRV), which are among the most densely populated regions in China, are subject to frequent flooding. In this study, the predictor importance analysis model was used to sort and select predictors, and five methods (multiple linear regression (MLR), decision tree (DT), random forest (RF), backpropagation neural network (BPNN), and convolutional neural network (CNN)) were used to predict the interannual variation of summer precipitation over the middle and lower reaches of the YRV. Predictions from eight climate models were used for comparison. Of the five tested methods, RF demonstrated the best predictive skill. Starting the RF prediction in December, when its prediction skill was highest, the 70-year correlation coefficient from cross validation of average predictions was 0.473. Using the same five predictors in December 2019, the RF model successfully predicted the YRV wet anomaly in summer 2020, although it had weaker amplitude. It was found that the enhanced warm pool area in the Indian Ocean was the most important causal factor. The BPNN and CNN methods demonstrated the poorest performance. The RF, DT, and climate models all showed higher prediction skills when the predictions start in winter than in early spring, and the RF, DT, and MLR methods all showed better prediction skills than the numerical climate models. Lack of training data was a factor that limited the performance of the machine learning methods. Future studies should use deep learning methods to take full advantage of the potential of ocean, land, sea ice, and other factors for more accurate climate predictions.


2021 ◽  
Vol 35 (4) ◽  
pp. 557-570
Author(s):  
Licheng Wang ◽  
Xuguang Sun ◽  
Xiuqun Yang ◽  
Lingfeng Tao ◽  
Zhiqi Zhang

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