Genetic Divergence and Chemotype Diversity in the Fusarium Head Blight Pathogen Fusarium poae

Abstract Background Fusarium head blight is a disease of global concern that reduces crop yields and renders grains unfit for consumption due to mycotoxin contamination. Fusarium poae is frequently associated with cereal crops showing symptoms of Fusarium head blight. While previous studies have shown F. poae isolates produce a range of known mycotoxins, including type A and B trichothecenes, fusarins and beauvericin, genomic analysis suggests that this species may have lineage-specific accessory chromosomes with secondary metabolite biosynthetic gene clusters awaiting description. Methods We examined the biosynthetic potential of 38 F. poae isolates from Eastern Canada using a combination of long-read and short-read genome sequencing and untargeted, high resolution mass spectrometry metabolome analysis of extracts from isolates cultured in multiple media conditions. Results A high-quality assembly of isolate DAOMC 252244 (Fp157) contained four core chromosomes as well as seven additional contigs with traits associated with accessory chromosomes. One of the predicted accessory contigs harbours a functional biosynthetic gene cluster containing homologs of all genes associated with the production of apicidins. Metabolomic and genomic analyses confirm apicidins are produced in 4 of the 38 isolates investigated and genomic PCR screening detected the apicidin synthetase gene APS1 in approximately 7% of Eastern Canadian isolates surveyed. Conclusions Apicidin biosynthesis is linked to isolate-specific putative accessory chromosomes in F. poae. The data produced here are an important resource for furthering our understanding of accessory chromosome evolution and the biosynthetic potential of F. poae.

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First Report of Fusarium poae Causing Fusarium Head Blight of Wheat in Georgia, U.S.A.

Plant Disease ◽

10.1094/pdis-08-20-1779-pdn ◽

2020 ◽

pp. PDIS-08-20-1779

Author(s):

B. Ghimire ◽

A. D. Martinez-Espinoza ◽

B. Ghimire ◽

B. C. Harrelson ◽

J. Youmans ◽

...

Keyword(s):

Fusarium Head Blight ◽

Head Blight ◽

First Report ◽

Fusarium Poae

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First Report of Fusarium poae Associated with Fusarium Head Blight in Wheat in Paraguay

Plant Disease ◽

10.1094/pdis-04-18-0692-pdn ◽

2019 ◽

Vol 103 (3) ◽

pp. 580 ◽

Cited By ~ 2

Author(s):

A. A. Arrúa ◽

J. Moura Mendes Arrua ◽

C. C. Cazal ◽

J. M. Iehisa ◽

Y. M. Reyes Caballero ◽

...

Keyword(s):

Fusarium Head Blight ◽

Head Blight ◽

First Report ◽

Fusarium Poae

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Fusarium Species Pathogenic to Barley and Their Associated Mycotoxins

Plant Disease ◽

10.1094/pdis.1999.83.7.667 ◽

1999 ◽

Vol 83 (7) ◽

pp. 667-674 ◽

Cited By ~ 92

Author(s):

B. Salas ◽

B. J. Steffenson ◽

H. H. Casper ◽

B. Tacke ◽

L. K. Prom ◽

...

Keyword(s):

South Dakota ◽

Fusarium Head Blight ◽

Fusarium Graminearum ◽

Fusarium Species ◽

Red River ◽

Limited Extent ◽

Head Blight ◽

Red River Valley ◽

Fusarium Poae ◽

Respective Species

Epidemics of Fusarium head blight (FHB) occurred on barley in Minnesota, North Dakota, and South Dakota from 1993 to 1998. The Red River Valley region was most severely impacted by the disease based on assessments of FHB severity in grain samples harvested from commercial fields. Fusarium graminearum was the primary pathogen causing these FHB epidemics. It comprised from 62 to 64% of all Fusarium species isolated from infected kernels from 1994 to 1996. Fusarium poae (range of isolation 13 to 20%),F. sporotrichioides (10 to 17%), and F. avenaceum (6 to 10%) also were isolated from barley kernels and were likely involved in causing some FHB infection, but to a very limited extent. All four Fusarium species were pathogenic on barley in inoculation tests conducted in both the greenhouse and the field. Mycotoxin screens were performed on barley spikes inoculated with the respective species in the greenhouse. Spikes infected with F. graminearum contained deoxynivalenol and 15-acetyldeoxyni-valenol; those infected with F. sporotrichioides contained T-2 toxin, HT-2 toxin, and T-2 tetraol; and those infected with F. poae contained nivalenol. Some isolates of F. poae also produced 15-acetoxyscirpenol and scirpentriol. Although F. graminearum and DON are recognized as the primary FHB pathogen and mycotoxin, respectively, in barley, the possible presence of other Fusarium species and mycotoxins should not be overlooked.

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Fusarium head blight (FHB) and Fusarium spp. on grain of spring wheat cultivars grown in Poland

Journal of Plant Protection Research ◽

10.1515/jppr-2015-0038 ◽

2015 ◽

Vol 55 (3) ◽

pp. 266-277 ◽

Cited By ~ 8

Author(s):

Leszek Lenc ◽

Grzegorz Czecholiński ◽

Dariusz Wyczling ◽

Tomasz Turów ◽

Arkadiusz Kaźmierczak

Keyword(s):

Fusarium Head Blight ◽

Spring Wheat ◽

Strong Positive Correlation ◽

Wheat Cultivars ◽

Head Blight ◽

Fusarium Equiseti ◽

Positive Correlation ◽

Fusarium Poae ◽

June July ◽

Moderately Resistant

AbstractEighteen spring wheat cultivars, recommended for commercial production in northern Poland, were assessed for Fusarium head blight (FHB) in natural non-epidemic conditions, from 2011 to 2013. Assessment was based on FHB incidence (proportion of heads with symptoms), disease severity (DS; proportion of bleached spikelets per head), proportion ofFusariumdamaged kernels (FDK), and spectrum ofFusariumspp. colonising the kernels. Fusarium head blight incidence and DS often differed significantly among cultivars and years. There was a strong positive correlation between FHB incidence and DS. Fusarium head blight incidence and DS were not correlated with the June–July temperatures, and were only occasionally correlated with the total June–July rainfall. There was a weak positive correlation between FHB incidence and proportion of FDK. There was a strong positive correlation between DS and proportion of FDK. The cultivar affected colonisation of kernels byFusariumspp.Fusarium poaewas the FHB pathogen isolated most often.Fusarium poaecolonised 6.0% of the kernels, on average, but up to 12.0% on individual cultivars. OtherFusariumspecies were less frequent:F. avenaceumin 5.6% of kernels,F. culmorumin 5.3%,F. tricinctumin 2.8%,F. graminearumin 1.5%, andF. sporotrichioidesin 1.2%.Fusarium equisetioccurred sporadically. The importance ofF. poaein the FHB complex is emphasised. All cultivars expressed ‘moderate FHB resistance’ if evaluated according to FHB incidence. Cultivars Arabella, Izera, Kandela, Monsun, Ostka Smolicka, and Struna expressed ‘moderate susceptibility’, and Bombona, Hewilla, Katoda, KWS Torridon, Łagwa, Nawra, Parabola, Radocha, SMH 87, Trappe, Tybalt, and Waluta expressed ‘susceptibility’ if evaluated by the proportion of FDK. Cultivars differed within the ‘moderately resistant’, ‘moderately susceptible’, and ‘susceptible’ categories. Cultivars Arabella, Izera, Kandela, Monsun, Ostka Smolicka, and Struna were the most promising and their resistance traits may be useful in FHB management.

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Fusarium head blight (FHB) and Fusarium populations in grain of winter wheat grown in different cultivation systems

Journal of Plant Protection Research ◽

10.1515/jppr-2015-0013 ◽

2015 ◽

Vol 55 (1) ◽

pp. 94-109 ◽

Cited By ~ 11

Author(s):

Leszek Lenc

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Production Systems ◽

Fusarium Species ◽

Cropping System ◽

Common Species ◽

Head Blight ◽

Fusarium Poae ◽

Conventional Systems ◽

Wheat Kernels

Abstract Fusarium head blight (FHB) incidence, and colonisation of grain by Fusarium species on winter wheat grown in organic, integrated, and conventional systems as well as in monoculture, were studied locally in Poland, from 2002 to 2010. Fusarium head blight incidence differed throughout the study years. It was found to occur the most where rainfall was highest and where rainfall was the most prolonged before, during, and after flowering of wheat. Fusarium head blight incidence was generally less on wheat grown organically than on wheat grown in other systems. In some years, FHB was noted more in monocultures than in other systems. Fusarium poae was the most common species of FHB populations in wheat kernels, followed by F. avenaceum and F. tricinctum. Other species which occurred more rarely or sporadically were: F. culmorum, F. equiseti, F. graminearum, F. langsethiae, F. oxysporum, and F. sporotrichioides. There were found to be significant effects of the cropping system on grain colonisation by Fusarium in some years. There was a positive correlation between FHB incidence and number of kernels colonised and damaged by Fusarium, in all four systems. Inferences were drawn concerning the effects of different procedures in different production systems and the possible value for controlling FHB

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Comparing Genetic Variation within Red Winter Wheat Populations with and without Image-Based Optical Sorter Selection

Al-Qadisiyah Journal For Agriculture Sciences (QJAS) (P-ISSN 2077-5822 E-ISSN 2617-1479) ◽

10.33794/qjas.vol9.iss2.82 ◽

2019 ◽

Vol 9 (2) ◽

pp. 266-277

Author(s):

Hussein M. Khaeim ◽

Anthony Clark ◽

Tom Pearson ◽

Dr. David Van Sanford

Keyword(s):

Genetic Variation ◽

Fusarium Head Blight ◽

Heading Date ◽

Fusarium Head Blight Resistance ◽

Gibberella Zeae ◽

Mass Selection ◽

Head Blight ◽

Red Winter Wheat ◽

Two Populations ◽

Head Scab

Head scab is historically a devastating disease affecting not just all classes of wheat but also barley and other small grains around the world. Fusarium head blight (FHB), or head scab, is caused most often by Fusarium graminearum (Schwabe), (sexual stage – Gibberella zeae) although several Fusarium spp. can cause the disease. This study was conducted to determine the effect of mass selection for FHB resistance using an image-based optical sorter. lines were derived from the C0 and C2 of two populations to compare genetic variation within populations with and without sorter selection. Our overall hypothesis is that sorting grain results in improved Fusarium head blight resistance. Both of the used wheat derived line populations have genetic variation, and population 1 has more than population 17. They are significantly different from each other for fusarium damged kernel (FDK), deoxynivalenol (DON), and other FHB traits. Although both populations are suitable to be grown for bulks, population 1 seems better since it has more genetic variation as well as lower FDK and DON, and earlier heading date. Lines within each population were significantly different and some lines in each population had significantly lower FDK and DON after selection using an optical sorter. Some lines had significant reduction in both FDK and DON, and some others had either FDK or DON reduction. Lines of population 1 that had significant reduction, were more numerous than in population 17, and FDK and DON reduction were greater.

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