scholarly journals Performance of an Automated Zika IgG Immunoassay in the Detection of Zika IgG Specific Antibodies—A Validation Approach in Samples from Prevalence Areas and Non-Endemic Countries

2020 ◽  
Vol 5 (2) ◽  
pp. 97
Author(s):  
Tina Laengin ◽  
Stephanie Augenstein ◽  
Elke Stadlbauer ◽  
Heike Girgnhuber ◽  
Mario Gloeck ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Cross Reactivity ◽  
Acute Stage ◽  
Igg Antibodies ◽  
Specific Antibodies ◽  
Diagnostic Samples ◽  
Diagnostic Sensitivity And Specificity ◽  
Blood Screening ◽  
Important Marker

The diagnosis of Zika virus infection is complicated and includes testing for nucleic acids and IgM and IgG antibodies, depending on the stage of infection. Zika IgG is an important marker of infection after the acute stage; however, IgG assays can lack specificity due to the similarities between Zika and other flaviviruses. In this study, the diagnostic sensitivity and specificity of the Elecsys® Zika IgG assay were assessed in 496 samples from Zika endemic regions, and specificity only was assessed in 1685 blood screening and diagnostic samples from Zika non-endemic regions. Cross-reactivity was also assessed against a panel of 202 potentially cross-reacting samples. The performance of the Elecsys® Zika IgG assay was compared with the anti-Zika virus ELISA IgG. In the samples from the Zika endemic regions, the Elecsys® Zika IgG assay had 92.88% (95% confidence interval 89.42–95.48) sensitivity and 100% specificity and in the samples from Europe the Elecsys® Zika IgG assay specificity was ≥99.62%. The Elecsys® Zika IgG assay was highly specific in samples from both prevalent and non-endemic regions.

scholarly journals Ability To Serologically Confirm Recent Zika Virus Infection in Areas with Varying Past Incidence of Dengue Virus Infection in the United States and U.S. Territories in 2016

2017 ◽  
Vol 56 (1) ◽  
Author(s):  
Nicole P. Lindsey ◽  
J. Erin Staples ◽  
Krista Powell ◽  
Ingrid B. Rabe ◽  
Marc Fischer ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Virus Infection ◽  
Dengue Virus ◽  
Zika Virus ◽  
Denv Infection ◽  
The United States ◽  
American Samoa ◽  
Cross Reactivity ◽  
Immunoglobulin M ◽  
Positive Results

ABSTRACTCross-reactivity within flavivirus antibody assays, produced by shared epitopes in the envelope proteins, can complicate the serological diagnosis of Zika virus (ZIKAV) infection. We assessed the utility of the plaque reduction neutralization test (PRNT) to confirm recent ZIKAV infections and rule out misleading positive immunoglobulin M (IgM) results in areas with various levels of past dengue virus (DENV) infection incidence. We reviewed PRNT results of sera collected for diagnosis of ZIKAV infection from 1 January through 31 August 2016 with positive ZIKAV IgM results, and ZIKAV and DENV PRNTs were performed. PRNT result interpretations included ZIKAV, unspecified flavivirus, DENV infection, or negative. For this analysis, ZIKAV IgM was considered false positive for samples interpreted as a DENV infection or negative. In U.S. states, 208 (27%) of 759 IgM-positive results were confirmed to be ZIKAV compared to 11 (21%) of 52 in the U.S. Virgin Islands (USVI), 15 (15%) of 103 in American Samoa, and 13 (11%) of 123 in Puerto Rico. In American Samoa and Puerto Rico, more than 80% of IgM-positive results were unspecified flavivirus infections. The false-positivity rate was 27% in U.S. states, 18% in the USVI, 2% in American Samoa, and 6% in Puerto Rico. In U.S. states, the PRNT provided a virus-specific diagnosis or ruled out infection in the majority of IgM-positive samples. Almost a third of ZIKAV IgM-positive results were not confirmed; therefore, providers and patients must understand that IgM results are preliminary. In territories with historically higher rates of DENV transmission, the PRNT usually could not differentiate between ZIKAV and DENV infections.

scholarly journals Sensitivity and Kinetics of an NS1-Based Zika Virus Enzyme-Linked Immunosorbent Assay in Zika Virus-Infected Travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile

2017 ◽  
Vol 55 (6) ◽  
pp. 1894-1901 ◽  
Author(s):  
Yaniv Lustig ◽  
Hana Zelena ◽  
Giulietta Venturi ◽  
Marjan Van Esbroeck ◽  
Camilla Rothe ◽  
...  
Keyword(s):  
Czech Republic ◽  
Immunosorbent Assay ◽  
Zika Virus ◽  
False Negative ◽  
Cross Reactivity ◽  
Diagnostic Methods ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Kinetics Of

ABSTRACT Serological diagnosis of Zika virus is challenging due to high cross-reactivity of Zika virus with other flavivirus antibodies. Recently, a Zika NS1-based enzyme-linked immunosorbent assay (ELISA) was developed and shown to be highly specific for Zika antibody detection; however, sensitivity was evaluated for only a small number of confirmed Zika-infected patients. In this study, we measured the sensitivity and kinetics of Zika IgM and IgG antibodies using the Zika NS1-based ELISA in 105 samples from 63 returning travelers infected with Zika virus (proven by PCR or neutralization assay) from Israel, Czech Republic, Italy, Belgium, Germany, and Chile. Zika virus IgM was detected from 2 to 42 days post-symptom onset (PSO) with an overall sensitivity of 79% in the first month and 68% until 2 months PSO, while IgG antibodies were detected from 5 days to 3 years PSO with 79% sensitivity. Interestingly, significant differences in IgM sensitivity and IgM detection period were observed between Israeli and European/Chilean Zika-infected travelers, adding to the complexity of Zika infection diagnosis and suggesting that other diagnostic methods should be complemented to reduce false-negative results.

scholarly journals Development of Envelope Protein Antigens To Serologically Differentiate Zika Virus Infection from Dengue Virus Infection

2017 ◽  
Vol 56 (3) ◽  
Author(s):  
Lakshmanane Premkumar ◽  
Matthew Collins ◽  
Stephen Graham ◽  
Guei-Jiun Alice Liou ◽  
Cesar A. Lopez ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Zika Virus ◽  
Envelope Protein ◽  
Population Level ◽  
Denv Infection ◽  
Cross Reactivity ◽  
Protein Antigens ◽  
Zikv Infection ◽  
Serological Tests

ABSTRACT Zika virus (ZIKV) is an emerging flavivirus that can cause birth defects and neurologic complications. Molecular tests are effective for diagnosing acute ZIKV infection, although the majority of infections produce no symptoms at all or present after the narrow window in which molecular diagnostics are dependable. Serology is a reliable method for detecting infections after the viremic period; however, most serological assays have limited specificity due to cross-reactive antibodies elicited by flavivirus infections. Since ZIKV and dengue virus (DENV) widely cocirculate, distinguishing ZIKV infection from DENV infection is particularly important for diagnosing individual cases or for surveillance to coordinate public health responses. Flaviviruses also elicit type-specific antibodies directed to non-cross-reactive epitopes of the infecting virus; such epitopes are attractive targets for the design of antigens for development of serological tests with greater specificity. Guided by comparative epitope modeling of the ZIKV envelope protein, we designed two recombinant antigens displaying unique antigenic regions on domain I (Z-EDI) and domain III (Z-EDIII) of the ZIKV envelope protein. Both the Z-EDI and Z-EDIII antigens consistently detected ZIKV-specific IgG in ZIKV-immune sera but not cross-reactive IgG in DENV-immune sera in late convalescence (>12 weeks postinfection). In contrast, during early convalescence (2 to 12 weeks postinfection), secondary DENV-immune sera and some primary DENV-immune sera cross-reacted with the Z-EDI and Z-EDIII antigens. Analysis of sequential samples from DENV-immune individuals demonstrated that Z-EDIII cross-reactivity peaked in early convalescence and declined steeply over time. The Z-EDIII antigen has much potential as a diagnostic antigen for population-level surveillance and for detecting past infections in patients.

scholarly journals First detection of Zika virus infection in a Croatian traveler returning from Brazil, 2016

2017 ◽  
Vol 11 (08) ◽  
pp. 662-667
Author(s):  
Tatjana Vilibic-Cavlek ◽  
Ljiljana Betica-Radic ◽  
Giulietta Venturi ◽  
Claudia Fortuna ◽  
Stjepan Djuricic ◽  
...  
Keyword(s):  
Zika Virus ◽  
High Titer ◽  
Disease Onset ◽  
Negative Control ◽  
Cross Reactivity ◽  
Immunofluorescence Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Grade ◽  
Igg Antibodies ◽  
Zikv Infection

In the last few years, several imported cases of Zika virus (ZIKV) infection were reported in European countries. We report the first imported ZIKV infection case in a Croatian traveler returning from Brazil. The patient presented with a low-grade fever, pruritic rash, general weakness, myalgia, arthralgia and edema of the legs and recovered completely within a week. ZIKV infection was confirmed by detection of IgM/IgG antibodies using enzyme-linked immunosorbent assay (ELISA) and confirmed by plaque-reduction neutralization test (PRNT). ZIKV IgM antibodies cross-reacted with dengue virus (DENV), West Nile virus (WNV) and tick-borne encephalitis virus (TBEV) in ELISA. In indirect immunofluorescence assay (IFA), IgM cross-reactivity was found only with DENV-3.  ZIKV IgG antibodies cross-reacted with DENV in both ELISA and IFA. PRNT for DENV was negative. Control serology performed on days 64 and 98 after disease onset showed a decline in cross-reactive heterologous DENV IgG antibodies compared to persistently high titer of homologous ZIKV IgG antibodies.

Cross reactivity of commercial anti-dengue immunoassays in patients with acute Zika virus infection

2017 ◽  
Vol 89 (8) ◽  
pp. 1477-1479 ◽  
Author(s):  
Alvina Clara Felix ◽  
Nathalia C. Santiago Souza ◽  
Walter M. Figueiredo ◽  
Angela A. Costa ◽  
Marta Inenami ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Cross Reactivity ◽  
Zika Virus Infection

scholarly journals Serological cross-reactivity using a SARS-CoV-2 ELISA test in acute Zika virus infection, Colombia

2020 ◽  
Vol 101 ◽  
pp. 191-193 ◽  
Author(s):  
Álvaro A. Faccini-Martínez ◽  
Ricardo Rivero ◽  
Evelin Garay ◽  
Alejandra García ◽  
Salim Mattar ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Elisa Test ◽  
Cross Reactivity ◽  
Zika Virus Infection
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