scholarly journals Infectious Virions of Bombyx Mori Latent Virus Are Incorporated into Bombyx Mori Nucleopolyhedrovirus Occlusion Bodies

Viruses ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 316 ◽  
Author(s):  
Keita Tsukui ◽  
Chihiro Yagisawa ◽  
Shota Fujimoto ◽  
Moe Ogawa ◽  
Ryuhei Kokusho ◽  
...  
Keyword(s):  
Life Cycle ◽  
Bombyx Mori ◽  
Plant Virus ◽  
Latent Virus ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Dna Virus ◽  
Virus Family ◽  
Positive Sense ◽  
Occlusion Bodies ◽  
Almost All

The Bombyx mori latent virus (BmLV) belongs to the unassigned plant virus family Tymoviridae and contains a positive-sense, single-stranded RNA genome. BmLV has infected almost all B. mori-derived cultured cell lines through unknown routes. The source of BmLV infection and the BmLV life cycle are still unknown. Here, we examined the interaction between BmLV and the insect DNA virus Bombyx mori nucleopolyhedrovirus (BmNPV). Persistent infection with BmLV caused a slight delay in BmNPV propagation, and BmLV propagation was enhanced in B. mori larvae via co-infection with BmNPV. We also showed that BmLV infectious virions were co-occluded with BmNPV virions into BmNPV occlusion bodies. We propose a new relationship between BmLV and BmNPV.

scholarly journals Genome Sequence of a Bombyx mori Nucleopolyhedrovirus Strain with Cubic Occlusion Bodies

2012 ◽  
Vol 86 (18) ◽  
pp. 10245-10245 ◽  
Author(s):  
Ruo-Lin Cheng ◽  
Yi-Peng Xu ◽  
Chuan-Xi Zhang
Keyword(s):  
Bombyx Mori ◽  
Genome Size ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
The Other ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Content Type ◽  
Typical Species ◽  
Occlusion Bodies

Bombyx morinucleopolyhedrovirus (BmNPV) is a typical species ofBaculoviridae. The complete genome sequence of a BmNPV strain with cubic occlusion bodies is reported here. The genome of this strain consists of 127,465 nucleotides with a G+C content of 40.36% and is 97.3% and 97.5% identical to those of BmNPV strain T3 andBombyx mandarinaNPV S1, respectively. Despite the abnormal polyhedra it forms, thepolyhedringene of the BmNPV cubic strain is 100% identical to those of the other two strains. Baculovirus repeated ORFs and homologous repeat regions cause the major differences in genome size of these BmNPV isolates.

First Detection of Tobacco Mosaic Virus in Tobacco Fields in Northern Lebanon

2020 ◽  
Vol 20 ◽  
Author(s):  
Rami Obeid ◽  
Elias Wehbe ◽  
Mohamad Rima ◽  
Mohammad Kabara ◽  
Romeo Al Bersaoui ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Viral Genome ◽  
Virus Genome ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Family ◽  
Crop Fields ◽  
Wide Range ◽  
Almost All ◽  
Das Elisa

Background: Tobacco mosaic virus (TMV) is the most known virus in the plant mosaic virus family and is able to infect a wide range of crops, in particularly tobacco, causing a production loss. Objectives: Herein, and for the first time in Lebanon, we investigated the presence of TMV infection in crops by analyzing 88 samples of tobacco, tomato, cucumber and pepper collected from different regions in North Lebanon. Methods: Double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), revealed a potential TMV infection of four tobacco samples out of 88 crops samples collected. However, no tomato, cucumber and pepper samples were infected. The TMV+ tobacco samples were then extensively analyzed by RT-PCR to detect viral RNA using different primers covering all the viral genome. Results and Discussion: PCR results confirmed those of DAS-ELISA showing TMV infection of four tobacco samples collected from three crop fields of North Lebanon. In only one of four TMV+ samples, we were able to amplify almost all the regions of viral genome, suggesting possible mutations in the virus genome or an infection with a new, not yet identified, TMV strain. Conclusion: Our study is the first in Lebanon revealing TMV infection in crop fields, and highlighting the danger that may affect the future of agriculture.

scholarly journals CAD integrated automatic recognition of weld paths

2021 ◽  
Author(s):  
Tuan Anh Tran ◽  
Andrei Lobov ◽  
Tord Hansen Kaasa ◽  
Morten Bjelland ◽  
Ole Terje Midling
Keyword(s):  
Life Cycle ◽  
Spatial Analysis ◽  
Product Life Cycle ◽  
Real Life ◽  
Automatic Recognition ◽  
Integrated Method ◽  
Weld Joints ◽  
Related Information ◽  
Almost All ◽  
Sequential Assembly

AbstractIn this paper, a CAD integrated method is proposed for automatic recognition of potential weld locations in large assembly structures predominantly comprised of weld joints. The intention is to reduce the total man-hours spent on manually locating, assigning, and maintaining weld-related information throughout the product life cycle. The method utilizes spatial analysis of extracted stereolithographic data in combination with available CAD functions to determine whether the accessibility surrounding a given intersection edge is sufficient for welding. To demonstrate the method, a system is developed in Siemens NX using their NXOpen Python API. The paper presents the application of the method to real-life use cases in varying complexity in cooperation with industrial partners. The system is able to correctly recognize almost all weld lines for the parts considered within a few minutes. Some exceptions are known for particular intersection lines located deep within notched joints and geometries weldable through sequential assembly, which are left as a subject to further works.

scholarly journals Development and reproduction of Podisus distinctus (Heteroptera: Pentatomidae) fed on larva of Bombyx mori (Lepidoptera: Bombycidae)

2004 ◽  
Vol 64 (2) ◽  
pp. 237-242 ◽  
Author(s):  
M. C. Lacerda ◽  
A. M. R. M. Ferreira ◽  
T. V. Zanuncio ◽  
J. C. Zanuncio ◽  
A. S. Bernardino ◽  
...  
Keyword(s):  
Biological Control ◽  
Life Cycle ◽  
Relative Humidity ◽  
Bombyx Mori ◽  
Insect Pests ◽  
Males And Females ◽  
Heteroptera Pentatomidae ◽  
Bombyx Mori L ◽  
Chemical Products ◽  
Coleoptera Tenebrionidae

Biological control has been reducing the use of chemical products against insect pests, specially predatory Pentatomidae. Species of this group can present high variations in their life cycle as a result of their diet. Thus, the objective of this research was to study nymph development and reproduction of Podisus distinctus (Stäl, 1860) (Heteroptera: Pentatomidae) fed on Bombyx mori L., 1758 (Lepidoptera: Bombycidae) larvae (T1), compared to those fed on Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae) (T2) and Musca domestica L., 1758 (Diptera: Muscidae) larvae (T3) at a temperature of 25 ± 0.5ºC, relative humidity of 70 ± 2%, and photophase of 12 h. Predators fed on B. mori showed duration of the nymph phase (18.68 ± 1.02) similar to those fed on T. molitor (18.32 ± 1.49). Pre-oviposition and oviposition periods and number of egg masses, besides eggs and nymphs per female, were higher with B. mori (5.83 ± 2.02; 15.00 ± 7.40; 8.42 ± 1.84; 296.69 ± 154.75; and 228.55 ± 141.04, respectively) while longevity of males and females of P. distinctus was 25.76 ± 16.15 and 35.00 ± 16.15 days with T. molitor, and 20.57 ± 13.60 and 23.46 ± 12.35 days with B. mori, respectively.

scholarly journals Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

2012 ◽  
Vol 86 (13) ◽  
pp. 7345-7359 ◽  
Author(s):  
J. Xue ◽  
N. Qiao ◽  
W. Zhang ◽  
R.-L. Cheng ◽  
X.-Q. Zhang ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Transcriptome Analysis ◽  
Host Cells ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Dynamic Interactions

Characterization of the open reading frame 7a from Bombyx mori nucleopolyhedrovirus

2012 ◽  
Vol 40 (2) ◽  
pp. 865-873
Author(s):  
Yong Liu ◽  
Feng Yu ◽  
Huiling Wu ◽  
Qing Cao ◽  
Yu Wu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Open Reading Frame ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Reading Frame
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