Survey and Characterization of Jingmen Tick Virus Variants

We obtained a Jingmen tick virus (JMTV) isolate, following inoculation of a tick pool with detectable Crimean-Congo hemorrhagic fever virus (CCHFV) RNA. We subsequently screened 7223 ticks, representing 15 species in five genera, collected from various regions in Anatolia and eastern Thrace, Turkey. Moreover, we tested specimens from various patient cohorts (n = 103), and canine (n = 60), bovine (n = 20) and avian specimens (n = 65). JMTV nucleic acids were detected in 3.9% of the tick pools, including those from several tick species from the genera Rhipicephalus and Haemaphysalis, and Hyalomma marginatum, the main vector of CCHFV in Turkey. Phylogenetic analysis supported two separate clades, independent of host or location, suggesting ubiquitous distribution in ticks. JMTV was not recovered from any human, animal or bird specimens tested. Near-complete viral genomes were sequenced from the prototype isolate and from three infected tick pools. Genome topology and functional organization were identical to the members of Jingmen group viruses. Phylogenetic reconstruction of individual viral genome segments and functional elements further supported the close relationship of the strains from Kosovo. We further identified probable recombination events in the JMTV genome, involving closely-related strains from Anatolia or China.

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Molecular characterization of small and medium segments of Crimean-Congo hemorrhagic fever virus in Turkey

Future Virology ◽

10.2217/fvl-2020-0003 ◽

2020 ◽

Vol 15 (4) ◽

pp. 247-254

Author(s):

Murat Karamese ◽

Erkan Ozmen ◽

Hakan Aydin ◽

Mehmet Ozkan Timurkan ◽

Mesud Fakirullahoglu

Keyword(s):

Infectious Disease ◽

Phylogenetic Analysis ◽

Phylogenetic Tree ◽

Molecular Characterization ◽

Hemorrhagic Fever ◽

Fever Virus ◽

Crimean Congo Hemorrhagic Fever ◽

Hemorrhagic Fever Virus ◽

Relationship Of

Aim: The objective was to investigate the genotypic relationship of S and M segments in Crimean-Congo hemorrhagic fever virus (CCHFV) by phylogenetic analysis in 25 patients from seven endemic cities in Turkey. Materials & methods: A total of 25 samples from patients with CCHF were included between 2012 and 2015. Phylogenetic tree analyses were inferred using MEGA version-6.0 and distances were calculated by Kimura’s 2-parameter. Results: Phylogenetic analysis showed that all isolated viruses (n = 25) were in the predicted clades such as clade V- Europe-1 regarding both S and M segments of the CCHFV. Conclusion: Further epidemiological, molecular and phylogenic studies should be performed in both reservoir animals/vectors and humans to determine the incidence of tick-borne infectious disease and to help to develop vaccines for prevention of the disease.

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Development and Characterization of SYBR Green I Based RT-PCR Assay for Detection of Omsk Hemorrhagic Fever Virus

Virologica Sinica ◽

10.1007/s12250-021-00389-5 ◽

2021 ◽

Author(s):

Ya-Nan Zhang ◽

Si-Qing Liu ◽

Cheng-Lin Deng ◽

Zhi-Ming Yuan ◽

Bo Zhang ◽

...

Keyword(s):

Hemorrhagic Fever ◽

Sybr Green ◽

Fever Virus ◽

Sybr Green I ◽

Rt Pcr ◽

Pcr Assay ◽

Hemorrhagic Fever Virus ◽

Omsk Hemorrhagic Fever

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Complete Genome Sequencing and Genetic Characterization of Alkhumra Hemorrhagic Fever Virus Isolated from Najran, Saudi Arabia

Intervirology ◽

10.1159/000362334 ◽

2014 ◽

Vol 57 (5) ◽

pp. 300-310 ◽

Cited By ~ 5

Author(s):

Tariq A. Madani ◽

Esam I. Azhar ◽

El-Tayeb M.E. Abuelzein ◽

Moujahed Kao ◽

Hussein M.S. Al-Bar ◽

...

Keyword(s):

Saudi Arabia ◽

Genome Sequencing ◽

Complete Genome ◽

Genetic Characterization ◽

Hemorrhagic Fever ◽

Fever Virus ◽

Complete Genome Sequencing ◽

Hemorrhagic Fever Virus

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Genetic Characterization of the M RNA Segment of Crimean Congo Hemorrhagic Fever Virus Strains, China

Emerging Infectious Diseases ◽

10.3201/eid0801.010087 ◽

2002 ◽

Vol 8 (1) ◽

pp. 50-53 ◽

Cited By ~ 51

Author(s):

Anna Papa

Keyword(s):

Genetic Characterization ◽

Hemorrhagic Fever ◽

Fever Virus ◽

Crimean Congo Hemorrhagic Fever ◽

Hemorrhagic Fever Virus ◽

Virus Strains

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Description of new chiactine-bearing sponges provides insights into the higher classification of Calcaronea (Porifera: Calcarea)

Zootaxa ◽

10.11646/zootaxa.4615.2.1 ◽

2019 ◽

Vol 4615 (2) ◽

pp. 201

Author(s):

ADRIANA ALVIZU ◽

JOANA R. XAVIER ◽

HANS TORE RAPP

Keyword(s):

New Species ◽

Phylogenetic Reconstruction ◽

Molecular Data ◽

Phylogenetic Study ◽

Two New Species ◽

Close Relationship ◽

New Material ◽

Relationship Of ◽

The Antarctic

A recent phylogenetic study revealed a close relationship between chiactine-bearing (family Achramorphidae, order Leucosolenida) and pugiole-bearing (order Baerida) calcaronean sponges as well as new putative taxa within Achramorphidae. In this study, we present a revision of chiactine-bearing sponges based on morphological re-examination of type material and recently collected specimens, in addition to new molecular data for the ribosomal 18S and C-region of the 28S. We provide re-descriptions for all known chiactine-bearing species, and further describe two new species from the Antarctic (Achramorpha antarctica sp. nov. and Megapogon schiaparellii sp. nov.) and two new species and a new genus from the Nordic Seas (Achramorpha ingolfi sp. nov. and Sarsinella karasikensis gen. nov. sp. nov.). The new phylogenetic reconstruction based on ribosomal 18S and C-region of the 28S confirms previous findings about the close relationship of some members of Baerida and the family Achramorphidae of the order Leucosolenida. However, new material and the addition of molecular data from the type species of both taxa would be required to formally propose changes at (sub-)ordinal levels within the classification of Calcaronean sponges.

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Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5

Virus Research ◽

10.1016/j.virusres.2010.08.023 ◽

2011 ◽

Vol 155 (1) ◽

pp. 61-68 ◽

Cited By ~ 21

Author(s):

Kentaro Yoshii ◽

Manabu Igarashi ◽

Kimihito Ito ◽

Hiroaki Kariwa ◽

Michael R. Holbrook ◽

...

Keyword(s):

Cdna Clone ◽

Hemorrhagic Fever ◽

Infectious Cdna Clone ◽

Fever Virus ◽

Hemorrhagic Fever Virus ◽

Omsk Hemorrhagic Fever

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Isolation and Molecular Characterization of Tick-Borne Relapsing Fever Borrelia Infecting Ornithodoros (Pavlovskyella) verrucosus Ticks Collected in Ukraine

The Journal of Infectious Diseases ◽

10.1093/infdis/jiz500 ◽

2019 ◽

Vol 221 (5) ◽

pp. 804-811 ◽

Cited By ~ 1

Author(s):

Serhii Filatov ◽

Aparna Krishnavajhala ◽

Brittany A Armstrong ◽

Alexander R Kneubehl ◽

Nathan C Nieto ◽

...

Keyword(s):

Unknown Etiology ◽

Bacterial Disease ◽

Relapsing Fever ◽

Health Practitioners ◽

Ribosomal Ribonucleic Acid ◽

Close Relationship ◽

Public Health Practitioners ◽

Relationship Of ◽

Insufficient Knowledge

Abstract Background Tick-borne relapsing fever (TBRF) is a neglected zoonotic bacterial disease known to occur on 5 continents. We report a laboratory-acquired case of TBRF caused by Borrelia caucasica, which is endemic in Ukraine and transmitted by Ornithodoros verrucosus ticks. Methods We isolated spirochetes and characterized them by partially sequencing the 16s ribosomal ribonucleic acid (rrs), flagellin (flaB), and deoxyribonucleic acid gyrase (gyrB) genes and conducting a phylogenetic analysis. Results These analyses revealed a close relationship of Ukrainian spirochetes with the Asian TBRF species, Borrelia persica. The taxonomic and nomenclature problems related to insufficient knowledge on the spirochetes and their vectors in the region are discussed. Conclusions Although these findings enhance our understanding of species identities for TBRF Borrelia in Eurasia, further work is required to address the neglected status of TBRF in this part of the world. Public health practitioners should consider TBRF and include the disease into differential diagnosis of febrile illnesses with unknown etiology.

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Characterization of Bacillus anthracis-Like Bacteria Isolated from Wild Great Apes from Côte d'Ivoire and Cameroon

Journal of Bacteriology ◽

10.1128/jb.00303-06 ◽

2006 ◽

Vol 188 (15) ◽

pp. 5333-5344 ◽

Cited By ~ 104

Author(s):

Silke R. Klee ◽

Muhsin Özel ◽

Bernd Appel ◽

Christophe Boesch ◽

Heinz Ellerbrok ◽

...

Keyword(s):

Bacillus Anthracis ◽

Protective Antigen ◽

Normal Growth ◽

Growth Conditions ◽

Penicillin G ◽

Molecular Features ◽

Close Relationship ◽

Resistance To Penicillin ◽

Relationship Of

ABSTRACT We present the microbiological and molecular characterization of bacteria isolated from four chimpanzees and one gorilla thought to have died of an anthrax-like disease in Côte d'Ivoire and Cameroon. These isolates differed significantly from classic Bacillus anthracis by the following criteria: motility, resistance to the gamma phage, and, for isolates from Cameroon, resistance to penicillin G. A capsule was expressed not only after induction by CO2 and bicarbonate but also under normal growth conditions. Subcultivation resulted in beta-hemolytic activity and gamma phage susceptibility in some subclones, suggesting differences in gene regulation compared to classic B. anthracis. The isolates from Côte d'Ivoire and Cameroon showed slight differences in their biochemical characteristics and MICs of different antibiotics but were identical in all molecular features and sequences analyzed. PCR and Southern blot analyses confirmed the presence of both the toxin and the capsule plasmid, with sizes corresponding to the B. anthracis virulence plasmids pXO1 and pXO2. Protective antigen was expressed and secreted into the culture supernatant. The isolates possessed variants of the Ba813 marker and the SG-749 fragment differing from that of classic B. anthracis strains. Multilocus sequence typing revealed a close relationship of our atypical isolates with both classic B. anthracis strains and two uncommonly virulent Bacillus cereus and Bacillus thuringiensis isolates. We propose that the newly discovered atypical B. anthracis strains share a common ancestor with classic B. anthracis or that they emerged recently by transfer of the B. anthracis plasmids to a strain of the B. cereus group.

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