Household Cases Suggest That Cats Belonging to Owners with COVID-19 Have a Limited Role in Virus Transmission

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for COVID-19 and spread rapidly following its emergence in Wuhan in 2019. Although cats are, among other domestic animals, susceptible to SARS-CoV-2 infection, little is known about their epidemiological role in the dynamics of a household infection. In this study, we monitored five cats for viral shedding daily. Each cat was confined with its COVID-19 positive owners in separate households. Low loads of viral nucleic acid were found in two cats, but only one developed anti-SARS-CoV-2 antibodies, which suggests that cats have a limited role in COVID-19 epidemiology.

Download Full-text

Positive results for patients with COVID-19 discharged from hospital in Chongqing, China

10.21203/rs.3.rs-17725/v1 ◽

2020 ◽

Author(s):

Wang Deng ◽

Tian-wen Guang ◽

Mei Yang ◽

Jian-rong Li ◽

De-peng Jiang ◽

...

Keyword(s):

Nucleic Acid ◽

Severe Acute Respiratory Syndrome ◽

Hospital Discharge ◽

Clinical Characteristics ◽

Clinical Evidence ◽

Viral Nucleic Acid ◽

Positive Results ◽

Nucleic Acid Tests ◽

At Home

Abstract Since December 2019 , more than 80 thousand patients with coronavirus disease 2019 (COVID-19) has been confirmed in China. With the increasing number of recovered patients, more attention should be paid to the follow-up of patients. In the study, 576 patients with COVID-19 discharged from hospital in Chongqing, China from January 24, 2020, to March 10, 2020 were evaluated by viral nucleic acid tests for SARS-CoV-2 to determine if they could be released from quarantine at home. Among the 576 patients, 61 patients (10.6%) had positive results for severe acute respiratory syndrome coronavirus 2. We aimed to analyze the demographics, clinical characteristics and treatment of 61 patients. Criteria for hospital discharge or discontinuation of quarantine would be updated with the progress of clinical evidence and experience accumulation.

Download Full-text

The diagnosis of hepatitis C viral infection

Orvosi Hetilap ◽

10.1556/oh.2014.29972 ◽

2014 ◽

Vol 155 (26) ◽

pp. 1019-1023

Author(s):

Judit Gervain

Keyword(s):

Hepatitis C ◽

Nucleic Acid ◽

Viral Infection ◽

Structural Changes ◽

Viral Infections ◽

Transient Elastography ◽

Viral Nucleic Acid ◽

Length Of Treatment ◽

Subtype B

The successful therapy of hepatitis C viral infection requires that the illness is diagnosed before the development of structural changes of the liver. Testing is stepwise consisting of screening, diagnosis, and anti-viral therapy follow-up. For these steps there are different biochemical, serological, histological and molecular biological methods available. For screening, alanine aminotransferase and anti-HCV tests are used. The diagnosis of infection is confirmed using real-time polymerase chain reaction of the viral nucleic acid. Before initiation of the therapy liver biopsy is recommended to determine the level of structural changes in the liver. Alternatively, transient elastography or blood biomarkers may be also used for this purpose. Differential diagnosis should exclude the co-existence of other viral infections and chronic hepatitis due to other origin, with special attention to the presence of autoantibodies. The outcome of the antiviral therapy and the length of treatment are mainly determined by the viral genotype. In Hungary, most patients are infected with genotype 1, subtype b. The polymorphism type that occurs in the single nucleotide located next to the interleukin 28B region in chromosome 19 and the viral polymorphism type Q80K for infection with HCV 1a serve as predictive therapeutic markers. The follow-up of therapy is based on the quantitative determination of viral nucleic acid according to national and international protocols and should use the same method and laboratory throughout the treatment of an individual patient. Orv. Hetil., 2014, 155(26), 1019–1023.

Download Full-text

Nucleic Acid Detection of Severe Acute Respiratory Syndrome Virus 2 (SARS-CoV-2): A Research in the Field of Health Economics

SSRN Electronic Journal ◽

10.2139/ssrn.3558020 ◽

2020 ◽

Author(s):

Yanfen Zeng ◽

Min Dou ◽

Yaqian Mao ◽

Yao Li ◽

Xijun Chen ◽

...

Keyword(s):

Nucleic Acid ◽

Health Economics ◽

Severe Acute Respiratory Syndrome ◽

Respiratory Syndrome Virus ◽

Nucleic Acid Detection

Download Full-text

Non-Viral Nucleic Acid Delivery: Key Challenges and Future Directions

Current Drug Delivery ◽

10.2174/156720111795256174 ◽

2011 ◽

Vol 8 (3) ◽

pp. 235-244 ◽

Cited By ~ 129

Author(s):

Mahmoud Elsabahy ◽

Adil Nazarali ◽

Marianna Foldvari

Keyword(s):

Nucleic Acid ◽

Nucleic Acid Delivery ◽

Viral Nucleic Acid ◽

Future Directions

Download Full-text

Clinical characteristics and outcomes of COVID-19 long-term nucleic acid positive patients

Technology and Health Care ◽

10.3233/thc-212921 ◽

2021 ◽

pp. 1-10

Author(s):

Xuan Zhu ◽

Xinxin Zhu ◽

Min Wang ◽

Fang Yang ◽

Zhibing Sun ◽

...

Keyword(s):

Nucleic Acid ◽

Clinical Characteristics ◽

Clinical Symptoms ◽

Control Group ◽

Viral Shedding ◽

Imaging Characteristics ◽

And Gender ◽

Acid Test ◽

Pulmonary Ct

OBJECTIVE: This study aimed to investigate the clinical characteristics and outcomes of coronavirus disease-19 (COVID-19) long-term nucleic acid positive patients (hereinafter referred to as CLTAPs). METHODS: Patients were recruited from the Xiaogan Central Hospital between 16 January 2020 and 28 March 2020. Among the 562 cases of patients with laboratory-identified COVID-19 infection by real-time polymerase chain reaction (qtPCR), 19 cases of COVID-19 patients with more than 41 days from the first to the last time of nucleic acid test were selected as the study group, and 76 cases of age- and gender-matched COVID-19 patients were selected as the control group (hereinafter referred to as C-CLTAPs). Demographic characteristics, clinical symptoms, laboratory examination and computed tomography (CT) imaging characteristics were retrospectively analyzed. RESULTS: On admission, among the 562 cases of patients with COVID-19, there were 398 cases of ordinary COVID-19 patients, 99 cases of severe COVID-19 patients and 99 cases of critical COVID-19 patients. CLTAPs had milder clinical symptoms and longer viral shedding time in comparison to C-CLTAPs. Compared to C-CLTAPs, CLTAPs had a lower infection index at admission. CLTAPs used less oxygen therapy and a higher proportion of hydroxychloroquine treatment in comparison to C-CLTAPs. In comparison to C-CLTAPs, CLTAPs showed slower pulmonary CT progression and faster pulmonary CT absorption. CONCLUSION: In this study, out of the 562 cases, we found 19 CLTAPs. The clinical differences between CLTAPs and C-CLTAPs were compared and analyzed. We hope that these finding can provide a theoretical basis for the treatment of CLTAPs.

Download Full-text

Are RNA-Based Tests Sufficient for COVID-19 Diagnosis? An Inspiration of Three Asymptomatic Cases

Cell Transplantation ◽

10.1177/0963689720985453 ◽

2021 ◽

Vol 30 ◽

pp. 096368972098545

Author(s):

Tao Hu ◽

Xiao Liu ◽

Qinan Yin ◽

Xingting Duan ◽

Li Yan

Keyword(s):

Computed Tomography ◽

Nucleic Acid ◽

Virus Infection ◽

Severe Acute Respiratory Syndrome ◽

The Other ◽

Computed Tomography Scan ◽

Health Authorities ◽

History Of ◽

Tomography Scan ◽

Nucleic Acid Tests

In this work, we discovered a new phenomenon—asymptomatic COVID-19 infection, or covert case, during the pandemic. All the 3 patients had a history of exposure, with no symptoms, and no abnormalities were found in computed tomography scan or lab tests. Except for case 2, the other patients’ severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) nucleic acid tests were negative. But their anti-SARS-COV-2 nucleocapsid antibody showed a dynamic trend, consistent with the process of virus infection and clearance. A growing number of asymptomatic or covert cases need more attention. Lack of surveillance may lead to another outbreak. We hope to demonstrate our cases to attract the attention of governments or health authorities that covert cases should be the focus as well.

Download Full-text