scholarly journals In pursuit of sensitivity: Lessons learned from viral nucleic acid detection and quantification on the raindance ddPCR platform

Methods ◽  
2021 ◽  
Author(s):  
Samuel Long
Keyword(s):  
Nucleic Acid ◽  
Lessons Learned ◽  
Nucleic Acid Detection ◽  
Viral Nucleic Acid ◽  
Detection And Quantification

The application of viral nucleic acid detection and lung CT in COVID-19 diagnosis

2020 ◽  
Author(s):  
Rui Hu
Keyword(s):  
Nucleic Acid ◽  
Clinical Symptoms ◽  
Virus Disease ◽  
False Negative ◽  
False Negative Rate ◽  
Diagnostic Methods ◽  
Nucleic Acid Detection ◽  
Viral Nucleic Acid ◽  
Early Medical Intervention ◽  
Lung Ct

The Corona Virus Disease 2019 (COVID-19) has the characteristics of fast propagation speed and strong pathogenicity and has attracted wide attention of people, medical workers, and researchers around the world. Accurate, rapid, and timely screening and diagnosis of COVID-19 is of great significance to control the development of the epidemic situation and save the lives of patients. Currently, the detection of viral nucleic acid and lung CT is the main screening and diagnostic methods of COVID-19. Nucleic acid detection has the advantages of fast, strong specificity and high sensitivity, but there is a certain false-negative rate. CT result of lung examination is visual, but it is not typical due to the uncertain time of clinical symptoms and the early medical intervention. Therefore, the diagnosis of COVID-19 should include a combination of epidemiological history, clinical symptoms, imaging, and laboratory tests.

A fully-integrated and automated testing device for PCR-free viral nucleic acid detection in whole blood

Lab on a Chip ◽  
2018 ◽  
Vol 18 (13) ◽  
pp. 1928-1935 ◽  
Author(s):  
Wenhan Liu ◽  
Jagotamoy Das ◽  
Adam H. Mepham ◽  
Carine R. Nemr ◽  
Edward H. Sargent ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Nucleic Acid ◽  
Disease Diagnosis ◽  
Automated Testing ◽  
Nucleic Acid Detection ◽  
Nucleic Acid Testing ◽  
Testing Device ◽  
Viral Nucleic Acid ◽  
Fully Integrated ◽  
Integrated Devices

Integrated devices for automated nucleic acid testing (NAT) are critical for infectious disease diagnosis to be performed outside of centralized laboratories.

scholarly journals Comparison of throat swabs and sputum specimens for viral nucleic acid detection in 52 cases of novel coronavirus (SARS-Cov-2) infected pneumonia (COVID-19)

2020 ◽  
Author(s):  
Chenyao Lin ◽  
Jie Xiang ◽  
Mingzhe Yan ◽  
Hongze Li ◽  
Shuang Huang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Detection Efficiency ◽  
Nucleic Acid Detection ◽  
Sample Collection ◽  
Pcr Assay ◽  
Viral Nucleic Acid ◽  
Detection Rates ◽  
Qrt Pcr ◽  
Positive Rate ◽  
Novel Coronavirus

AbstractBackgroundIn December 2019, a novel coronavirus (SARS-CoV-2) infected pneumonia (COVID-19) occurred in Wuhan, China. Diagnostic test based on real-time reverse transcription polymerase chain reaction assay (qRT-PCR) was the main means of confirmation, and sample collection was mostly throat swabs, which was easy to miss the diagnosis. It is necessary to seek specimen types with higher detection efficiency and accuracy.MethodsPaired specimens of throat swabs and sputum were obtained from 54 cases, and RNA was extracted and tested for 2019-nCoV (equated with SARS-CoV-2) by qRT-PCR assay.ResultsThe positive rates of 2019-nCoV from sputum specimens and throat swabs were 76.9% and 44.2%, respectively. Sputum specimens showed a significantly higher positive rate than throat swabs in detecting viral nucleic acid using qRT-PCR assay (P=0.001).ConclusionsThe detection rates of 2019-nCoV from sputum specimens are significantly higher than throat swabs. We suggest that sputum would benefit for the detection of 2019-nCoV in patients who produce sputum. The results can facilitate the selection of specimens and increase the accuracy of diagnosis.

scholarly journals A Research on the Results of Viral Nucleic Acid Tests and CT Imaging Variation of Patients with COVID-19

2020 ◽  
Author(s):  
Meng Xu ◽  
Xun Liu ◽  
Chuhong Su ◽  
Yuping Zeng ◽  
Jinqian Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Recovery Period ◽  
Ct Imaging ◽  
Nucleic Acid Detection ◽  
Test Results ◽  
Viral Nucleic Acid ◽  
Nucleic Acid Test ◽  
Acid Test ◽  
Pulmonary Ct ◽  
Nucleic Acid Tests

AbstractBackgroundCoronavirus disease 2019 (COVID-19) has become a global health problem. We aim to investigate the changes in the results of viral nucleic acid tests on pharyngeal swabs and feces of patients with COVID-19 and CT imaging of lungs as the disease progresses.MethodsSeven patients with COVID-19 in the third affiliated hospital of Sun Yat-sen University Yuedong Hospital were retrospectively enrolled with clinical features, including imaging staging, and performance characteristics of viral nucleic acid test results of pharyngeal swabs and feces. The dynamic changes of these features were observed during hospitalization, and therapeutic effect and prognosis of patients were evaluated.ResultsThe results of seven cases with COVID-19 were positive for viral nucleic acid tests on pharyngeal swabs early after the onset of symptoms, and then turned negative; while the results of viral nucleic acid tests on feces were persistently positive in the mid-term clinical treatment and recovery period. And the viral nucleic acid test results were capricious in three cases. Pulmonary CT imaging showed characteristic changes in early, advanced and recovery phases.ConclusionThe application of viral nucleic acid detection and pulmonary CT imaging can be used for screening of suspected cases. Fecal nucleic acid test should be recommended as the reference of discharge standard, in order to minimize the risk of transmission from digestive tract.

Advances in Nucleic Acid Detection and Quantification

2009 ◽  
Vol 37 (2) ◽  
pp. e1-e4 ◽  
Author(s):  
Ian C. Kavanagh ◽  
Simon C. Baker
Keyword(s):  
Nucleic Acid ◽  
Molecular Biology ◽  
Single Molecule ◽  
Single Cells ◽  
Genetic Research ◽  
Detection Methods ◽  
Nucleic Acid Detection ◽  
British Library ◽  
Introductory Session ◽  
Detection And Quantification

The last decade has seen many changes in molecular biology at the bench, as we have moved away from a primary goal of cataloguing genes and mRNA towards techniques that detect and quantify nucleic acid molecules even within single cells. With the invention of the polymerase chain reaction (PCR), a nucleic acid sequence could now be amplified to generate a large number of identical copies, and this launched a new era in genetic research. PCR has developed in parallel to fluorescent hybridization probing to provide low-, medium- and high-throughput detection methods. However, PCR and hybridization detection have significant drawbacks as long-term solutions for routine research and diagnostics assays. Therefore many novel methods are being developed independently, but as yet no one technique has emerged as a clear replacement for PCR, microarrays or even sequencing. In order to examine the technological horizon in this area, around 90 delegates assembled at Hinxton Hall, Cambridge, U.K. on 28 and 29 October 2008 for a Biochemical Society/Wellcome Trust Focused Meeting sponsored by Thermo Fisher Scientific and the British Library. The title of the meeting was ‘Advances in Nucleic Acid Detection and Quantification’, and the primary aim was to bring together scientists from different disciplines who nevertheless are trying to develop reliable methods for the quantification or detection of RNA and DNA molecules. This meant that physical and organic chemists, microbial ecologists and clinicians appeared alongside molecular biologists. An introductory session on general nucleic acid detection technologies was initiated with a fascinating insight into single-molecule, singlecell hybridization from Professor Sir Edwin Southern. This served as an ideal base for sessions on single-cell molecular biology and an examination of current applications of emerging technology. This issue of Biochemical Society Transactions contains some of the papers prepared by speakers at the meeting, and highlights not only how PCR and microarrays are already being replaced, but also which methods are likely to replace them.

scholarly journals Estimation of conjunctival swab and nasopharyngeal swab specimens for viral nucleic acid detection in Coronavirus disease 2019 patients to compare the viral load

2021 ◽  
Vol 4 ◽  
pp. 2
Author(s):  
Jaya Kaushik ◽  
Vikas Marwah ◽  
Ankita Singh ◽  
Y. V. K. Chaitanya ◽  
Rajeev Mohan Gupta ◽  
...  
Keyword(s):  
Viral Load ◽  
Nucleic Acid ◽  
Statistical Correlation ◽  
Nucleic Acid Detection ◽  
Nasopharyngeal Swab ◽  
Rt Pcr ◽  
Viral Nucleic Acid ◽  
Ocular Manifestations ◽  
Conjunctival Swab ◽  
Polymerase Chain

Objectives: The purpose of the study was to detect the presence of viral ribonucleic acid of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in conjunctival swab along with nasopharyngeal swab specimens of Coronavirus disease 2019 (COVID-19) patients. Material and Methods: Thirty COVID-19 patients with at least one sample positive for real-time reverse transcription-polymerase chain reaction for SARS-CoV-2 in nasopharyngeal swab with the presence or absence of ocular manifestations were included in the study. The conjunctival swab along with nasopharyngeal swab of each patient was collected and sent to microbiology lab for evaluation and analysis of viral nucleic acid to assess the viral load. Results: Out of 30 patients, 21 patients (70%) were males and the remaining nine patients (30%) were females. Mean age of the patients in the study was 44.80 ± 15.37 years. One patient had conjunctivitis as ocular manifestation. Two (6.7%) out of 30 patients were positive for RT-PCR SARS-CoV-2 in the conjunctival swab. There was no statistical correlation between nasopharyngeal swab and conjunctival swab positivity using Pearson’s correlation coefficient (r) = 0.010; P = 0.995 (>0.05). Conclusion: The results of the study revealed that SARS-CoV-2 can also be detected in conjunctival swabs of confirmed cases of COVID-19 patients. Although, in comparison to nasopharyngeal and throat swabs the rate of detection of SARS-CoV-2 in conjunctival swabs is relatively less, still diligent care and precautions should be practiced during the ophthalmic evaluation of COVID-19 patients.

Nucleic acid detection and quantification in the developing world

2009 ◽  
Vol 37 (2) ◽  
pp. 419-423 ◽  
Author(s):  
Jim Huggett ◽  
Clare Green ◽  
Alimuddin Zumla
Keyword(s):  
Nucleic Acid ◽  
Developing World ◽  
Rapid Diagnosis ◽  
Nucleic Acid Amplification ◽  
Resource Poor Setting ◽  
Nucleic Acid Detection ◽  
Relevant Research ◽  
The West ◽  
Resource Poor ◽  
Detection And Quantification

Techniques using nucleic acid amplification have not had the same amount of impact on research and clinical diagnosis in the developing world as that observed in the West. This is unsurprising when the costs and infrastructure required to perform nucleic acid amplification are considered. Despite this, nucleic acid amplification is being increasingly used in both research and diagnosis in countries such as Zambia and Tanzania. Scientific research in the developing world is made possible through the support and development of the necessary laboratory infrastructure and the establishment of special transport for the reagents and samples. This has enabled world-leading country-relevant research to be performed by local scientists on subjects ranging from rapid diagnosis of infectious diseases to measuring the RNA gene expression in an immune response. Concomitantly, the challenge presented by the need for tests that are more appropriate for a resource-poor setting has led to a number of newer methodologies for nucleic acid detection, which can be tailored to be performed in the field without the need for training in molecular biology. As nucleic acid amplification techniques become both simpler and cheaper, their impact is likely to play an increasingly crucial role in research and diagnosis in the developing world.

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