scholarly journals Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2413
Author(s):  
Martina Pavletić ◽  
Marija Mazor ◽  
Mate Lerga ◽  
Tatjana Mileta ◽  
Jelena Železnjak ◽  
...  

During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.

Author(s):  
Anna Burak ◽  
Justyna Antoniewicz ◽  
Elżbieta Malinowska ◽  
Katarzyna Cierzniakowska

Abstract Introduction. Hospital emergency departments (ED) are places of the highest aggression rates towards staff. In Poland, in the case of violent behavior personnel can exercise the right to legal protection reserved to public officials. To be able to exercise it, personnel should document the course of violent behavior they were subject to. Aim. The aim of the study was to examine whether the staff of ED document violent behavior they are subject to in the workplace and to answer the question: what factors are they taking into account while reporting or not violent behavior incidents. Methods. The study was conducted among medical personnel of six hospital ED in Poland. A total of 282 health care professionals took part in the study. The diagnostic survey method was applied. The data was compiled using Statistica PL and Microsoft Excel software. Results. 43.3% of respondents always document cases of violent behavior and 5.3% never do it. The personnel document aggression mainly for the purpose of their own safety - 44.8%. 40% of respondents who do not document acts of aggression consider it pointless. Conclusions. Cases of aggression are registered in medical records by nearly every second person of medical professional. The reasons for documenting cases of aggression include: concern for own safety, procedures in force in the unit, fear of further consequences.


2020 ◽  
Vol 37 (2) ◽  
pp. 190-200
Author(s):  
Anna Burak ◽  
Katarzyna Cierzniakowska ◽  
Aleksandra Popow

Aim: To assess the incidence of diagnoses related to alcohol use in the population of homeless people admitted to hospital emergency departments (EDs). Material and method: Data were analysed from three hospitals concerning stays of homeless people in three EDs in Bydgoszcz, Poland, in 2013–2015; 3133 stays were identified. The data were compiled using Microsoft Excel and Statistica 10 statistical software. Results: At the time of admission to EDs, 31% of homeless people were considered to be under the influence of alcohol. Diagnoses related to alcohol use accounted for 25% of all diagnoses. The average blood alcohol concentration in the patients was 2.97 per mille. The average blood alcohol concentration in the group of men was significantly higher than that in the group of women ( p = 0.015). The average length of stay in the ED of patients under the influence of alcohol was significantly longer ( p < 0.0001) than among sober patients. Conclusions: Homeless people under the influence of alcohol account for a third of the population of homeless patients admitted to hospital emergency departments, while alcohol-related ICD-10 diagnoses account for a fourth of all diagnoses in these patients. Homeless patients under the influence of alcohol stay longer in hospital emergency departments than do sober homeless people, which may translate into more frequent acts of aggression towards medical personnel. In Poland there are no systemic ED-level solutions as regards dealing with homeless patients for whom alcohol dependence is in many cases a reality.


1986 ◽  
Vol 2 (1-4) ◽  
pp. 116-117
Author(s):  
Attila Csepanyi ◽  
Mihaly Sebestyen

The first emergency department in Hungary was established in Hetenyi Geza County Hospital in 1976. It was organized as part of a research program commissioned by the Ministry of Health. Initially, the hospital had 1400 beds which have now increased to 2,000.The Emergency Department is located on the ground floor of a new building and occupies 1,100 square meters of surface. The operation of the department is divided into three main areas.


2015 ◽  
Vol 28 (6) ◽  
pp. 807-815 ◽  
Author(s):  
E. S. J. van Gils-van Rooij ◽  
C. J. Yzermans ◽  
S. M. Broekman ◽  
B. R. Meijboom ◽  
G. P. Welling ◽  
...  

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