scholarly journals Nitrogen Removal Characteristics and Comparison of the Microbial Community Structure in Different Anaerobic Ammonia Oxidation Reactors

Water ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 230 ◽  
Author(s):  
Liqiu Zhang ◽  
Wei Lv ◽  
Shugeng Li ◽  
Zhongxuan Geng ◽  
Hainan Yao
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Nitrogen Removal ◽  
Microbial Community Structure ◽  
Ammonia Oxidation ◽  
Settling Tank ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Start Up ◽  
Anaerobic Ammonia Oxidation

Nitrogen removal characteristics and the comparison of the microbial community structure were investigated in different anaerobic ammonia oxidation (Anammox) reactors: an anaerobic sequencing batch reactor (ASBR) and a biofilter reactor. The Anammox systems were inoculated with sludge from the second settling tank of a wastewater treatment plant in Guangzhou, China. After successful start up of Anammox, the microbial community structure of different Anammox reactors were studied through high-throughput sequencing. The results showed that anaerobic ammonium oxidation in the ASBR reactor could successfully start up after 134 days, while Anammox in the biofilter could start up after 114 days. In both systems, total nitrogen removal was at 80% after more than 200 days of operation. The diversity of denitrifying microorganisms was high in both reactors, with Planctomycetes as the main taxa. Anammox bacteria belonging to the genera Candidatus Anammoxoglobus and Kuenenia, were dominant in the ASBR, while all three genera of Candidatus, Anammoxoglobus, Kuenenia, and Brocadia, could be detected in the biofilter reactor. Therefore, the biofilter starts up faster than the ASBR, and contains richer species, which makes it more suitable to domesticate Anammox bacteria.

scholarly journals Anaerobic Ammonia Oxidation in the Presence of Nitrogen Oxides (NOx) by Two Different Lithotrophs

2002 ◽  
Vol 68 (11) ◽  
pp. 5351-5357 ◽  
Author(s):  
Ingo Schmidt ◽  
Cristian Hermelink ◽  
Katinka van de Pas-Schoonen ◽  
Marc Strous ◽  
Huub J. op den Camp ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Ammonia Oxidation ◽  
Mixed Population ◽  
Natural Environments ◽  
Ammonia Oxidizers ◽  
Oxidation Activity ◽  
Anaerobic Ammonia Oxidation ◽  
N Compounds

ABSTRACT The anaerobic ammonia-oxidizing activity of the planctomycete Candidatus “Brocadia anammoxidans” was not inhibited by NO concentrations up to 600 ppm and NO2 concentrations up to 100 ppm. B. anammoxidans was able to convert (detoxify) NO, which might explain the high NO tolerance of this organism. In the presence of NO2, the specific ammonia oxidation activity of B. anammoxidans increased, and Nitrosomonas-like microorganisms recovered an NO2-dependent anaerobic ammonia oxidation activity. Addition of NO2 to a mixed population of B. anammoxidans and Nitrosomonas induced simultaneous specific anaerobic ammonia oxidation activities of up to 5.5 mmol of NH4 + g of protein−1 h−1 by B. anammoxidans and up to 1.5 mmol of NH4 + g of protein−1 h−1 by Nitrosomonas. The stoichiometry of the converted N compounds (NO2 −/NH3 ratio) and the microbial community structure were strongly influenced by NO2. The combined activity of B. anammoxidans and Nitrosomonas-like ammonia oxidizers might be of relevance in natural environments and for technical applications.

Research Progress on the Anammox Technology

2011 ◽  
Vol 347-353 ◽  
pp. 2015-2020 ◽  
Author(s):  
Feng Xun Tan ◽  
Min Huang ◽  
Dao Ji Wu ◽  
Zhao Liang Zhu
Keyword(s):  
Nitrogen Removal ◽  
Ammonia Oxidation ◽  
Carbon Sources ◽  
Research Progress ◽  
Biological Nitrogen Removal ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Physiological And Biochemical Characteristics ◽  
Anaerobic Ammonia Oxidation ◽  
Biological Nitrogen

With the continuing phenomenon of eutrophication, the pollution of nitrogen has caused wide concern. Lack of organic carbon sources is always considered to be a key problem for nitrogen removal efficiency and cost when conventional biological nitrogen removal process was used to the treatment of ammonium-rich wastewater, but the anaerobic ammonium oxidation（ANAMMOX）can solve this problem .Anammox has so many advantages that it has good prospects in the biological nitrogen removal in wastewater. Anammox bacteria grows slowly, has a long generation time, and is susceptible to the environmental conditions. DO, Temperature, pH and organic can impact the process of anammox, so it is necessary to take careful methods to cultivate Anammox biomass. Depending on the purpose of improve water quality, appropriate treatment reactors and seed sludge should be selected to start ANAMMON process. The discovery of anaerobic ammonia oxidation, the reaction mechanism, influence application, the enrichment of anammox bacteria, physiological and biochemical characteristics of anammox bacteria, the start of anaerobic ammonia oxidation are reviewed in this paper.

scholarly journals Achieving fast start-up of anammox process by promoting the growth of anammox bacteria with FeS addition

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Chunzhen Zou ◽  
Beibei Guo ◽  
Xuming Zhuang ◽  
Liying Ren ◽  
Shou-Qing Ni ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal ◽  
Nitrite Reductase ◽  
Removal Rate ◽  
Anammox Bacteria ◽  
Gene Encoding ◽  
Start Up ◽  
Fast Start ◽  
The Difference ◽  
Anammox Process

Abstract The effects of FeS on nitrogen removal performance and microbial community of anammox process were studied. During the start-up period, the removal efficiencies of nitrite and total nitrogen were significantly improved by FeS. The addition of FeS increased the content of iron ions in the reactor and promoted the synthesis of heme c, which was involved in the formation of various enzymes. Compared with the control, the abundance of anammox bacteria in the FeS reactor was increased by 29%, and the expression level of the nirS gene (encoding cd1 type nitrite reductase containing heme) was nearly doubled. The content of nitrite reductase (ammonia-forming) in the community was increased by 26.4%. The difference in functional bacteria and enzyme contents in the microbial community resulted in a difference in nitrogen removal rate (NRR) between the two reactors. High-throughput results indicated that FeS increased the richness and diversity of microbial community and enhanced the metabolic function of the microbial community. The addition of FeS did not change the dominant position of Ca. Kuenenia in both reactors. But the relative abundance of heterotrophic denitrifying bacteria was reduced with FeS, which may be related to the inhibition effect of S2− produced by FeS.

Microbial community structure and occurrence of diverse autotrophic ammonium oxidizing microorganisms in the anammox process

2010 ◽  
Vol 61 (11) ◽  
pp. 2723-2732 ◽  
Author(s):  
H. Bae ◽  
Y.-C. Chung ◽  
J.-Y. Jung
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Fragment Length Polymorphism ◽  
Amoa Gene ◽  
Continuous Operation ◽  
Anaerobic Sludge ◽  
Anammox Bacteria ◽  
Ammonia Oxidizing Bacteria ◽  
Anammox Process

The enrichment of anaerobic ammonium oxidizing (anammox) bacteria using an upflow anaerobic sludge bioreactor was successfully conducted for 400 days of continuous operation. The bacterial community structure of anammox bioreactor included Proteobacteria (42%), Chloroflexi (22%), Planctomycetes (20%), Chlorobi (7%), Bacteroidetes (5%), Acidobacteria (2%), and Actinobacteria (2%). All clones of Planctomycetes were affiliated with the anammox bacteria, Planctomycete KSU-1 (AB057453). The presence and diversity of ammonia oxidizing bacteria (AOB) and archaea (AOA) were identified by terminal restriction fragment length polymorphism (T-RFLP) based on the amoA gene sequences. The AOB in anammox bioreactor were affiliated with the Nitrosomonas europaea cluster. The T-RFLP result of AOA showed the diverse microbial community structure of AOA with three terminal restriction fragments (T-RFs).

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