Development of the quantitative test methods to determine the number of flavonoids in flowering shoots of semi-soft hawthorn

2020 ◽  
pp. 28-34
Author(s):  
Vladimir Kurkin ◽  
Olga Pravdivtseva ◽  
Ilnur Shaikhutdinov ◽  
Anastasiya Kretova ◽  
Nadezhda Volkova

Flowers, fruits and shoots of various genus Hawthorn (Crataegus L.) species are used in domestic and foreign medical practice as cardiotonic agents. It is also possible to use hawthorn raw material for the prevention of cardiovascular system diseases among medical and pharmaceutical personnel. We consider semi-soft hawthorn Crataegus submollis Sarg. to be a prospective raw material species as its flowering shoots are chemically similar to the red-haw hawthorn flowers. We developed the quantitative test method to determine the number of flavonoids expressed as hyperoside in flowering shoots of semi-soft hawthorn using differential spectrophotometry with an analytical wavelength of 412 nm.

2004 ◽  
Vol 87 (2) ◽  
pp. 429-434
Author(s):  
Norman Miner ◽  
Valerie Harris ◽  
Sara Stumph ◽  
Amanda Cobb ◽  
Jennifer Ortiz

Abstract Tests were conducted by a Task Force on Disinfectant Test Methods that was appointed to investigate controversies regarding the accuracy of AOAC test methods for disinfectants as presented in AOAC's Official Methods of Analysis, Chapter 6. The general principles for new and improved AOAC tests are discussed, and a disinfectant test using microbes labeled onto a polyester fiber surface is described. The quantitative test measures the survival of test microbes as a function of exposure time as well as the exposure conditions required to kill 6 log10 of the test microbes. The time required was similar to that for the kinetics of the kill of Bacillus subtilis-labeled cylinders as tested by methods of the AOAC Sporicidal Test 966.04.


TAPPI Journal ◽  
2009 ◽  
Vol 8 (6) ◽  
pp. 24-28
Author(s):  
CORY JAY WILSON ◽  
BENJAMIN FRANK

TAPPI test T811 is the specified method to ascertain ECT relative to box manufacturer’s certification compliance of corrugated fiberboard under Rule 41/ Alternate Item 222. T811 test sample heights were derived from typical board constructions at the time of the test method’s initial development. New, smaller flute sizes have since been developed, and the use of lighter weight boards has become more common. The T811 test method includes sample specifications for typical A-flute, B-flute, and C-flute singlewall (and doublewall and triplewall) structures, but not for newer thinner E-flute or F-flute structures. This research explores the relationship of ECT sample height to measured compressive load, in an effort to determine valid E-flute and F-flute ECT sample heights for use with the T811 method. Through this process, it identifies challenges present in our use of current ECT test methods as a measure of intrinsic compressive strength for smaller flute structures. The data does not support the use of TAPPI T 811 for ECT measurement for E and F flute structures, and demonstrates inconsistencies with current height specifi-cations for some lightweight B flute.


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