scholarly journals Evaluation of nutritional amendments and fungicides against brown leaf spot of rice

2021 ◽  
pp. 86-94
Author(s):  
Fiza Mohsin ◽  
Safdar Ali ◽  
Muhammad Usman Ghani ◽  
Waqas Ashraf ◽  
Muhammad Ahmad Zeshan ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Disease Severity ◽  
Leaf Spot ◽  
Maximum Growth ◽  
Colony Growth ◽  
Growth Enhancement ◽  
Growth Reduction ◽  
Causal Organism ◽  
Helminthosporium Oryzae

Helminthosporium oryzae, the causal organism of bacterial leaf spot (BLS) has brought a tremendous loss of rice crop in the field. Rice seedlings of four commercial varieties (Nayab basmati, Kiran 434, Super basmati, and Pak 386) were screened against BLS in the field by using a randomized complete block design (RCBD). The present experiment was encompassed the evaluation of fermented farmyard manure, leaf manure, Boron, and NPK solution against BLS. The effect of different fungicides (Score, Flumax, Melodyduo, and Polyram) was also checked on the mycelial growth of Helminthosporium oryzae in-vitro and in-vivo. The results showed that Pak 386 was highly resistant with 17.22% disease severity and Super Basmati was found moderately resistant with 22.31% disease severity; variety Kisan 434 was found moderately susceptible (24.15%) and Nayab Basmati (24.82%) was susceptible. Among all NPK solutions showed the best results in growth enhancement, plant vigor as well as reduction in disease severity followed by FFYM, boron and leaf manure. Under in-vitro conditions, all the fungicides performed best at 150 ppm in terms of colony growth reduction. The maximum growth inhibition was recorded in Score followed by Flumax, Melodyduo, and Polyram. Score gave a significant reduction in BLS disease severity and maximized the agronomic attributes. The fungicides performed best at 150 ppm in terms of colony growth reduction. The maximum growth inhibition was recorded in Score followed by Flumax, Melodyduo, and Polyram. Score gave a significant reduction in BLS disease severity and maximized the agronomic attributes. Keywords: HBV, HCV, ICT, PCR, Risk factors, Mansehra

In-vitro and greenhouse management of banded leaf and sheath blight (BLSB) of maize, at Rampur, Chitwan, Nepal

2020 ◽  
Vol 5 (6) ◽  
Author(s):  
GC Sagar ◽  
Manandahar HK ◽  
Shrestha S ◽  
Bhusal K
Keyword(s):  
Growth Inhibition ◽  
Disease Severity ◽  
Block Design ◽  
Maximum Growth ◽  
Separate Experiment ◽  
Root Weight ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Pathology Laboratory

The research included in-vitro experiments on the effect of the fungal antagonist (Trichoderma spp.) and fungicide (carbendazim) against BLSB pathogen (Rhizoctonia solani); and greenhouse experiment for the management of BLSB using antagonists (Trichoderma and Pseudomonas) and a fungicide (carbendazim) in different combinations. The in-vitro and greenhouse experiments were done at the plant pathology laboratory and greenhouse of Agriculture and Forestry University, Rampur, Chitwan. Of the isolates of Trichoderma evaluated against the BLSB pathogen in dual culture, the Trichoderma isolated from between healthy and diseased maize plants gave the maximum growth inhibition percent of the pathogen at 24 (19.86±0.52), 48 (51.63±0.40) and 72hours (72.78±1.11). Carbendazim at 10 ppm completely inhibited the growth of both the pathogen and Trichoderma on PDA medium, while at 1 ppm, the growth inhibition of the pathogen was (54.22±0.89) percent and of Trichoderma was (50.22±3.11) percent. The management experiment in a glasshouse with seven treatments was done in a randomized complete block design with three replications. Carbendazim alone gave the highest disease control (59.73%) with the lowest disease severity (28.67±0.67) and AUDPC per day (803.33±33.33). A combination of treatments alternately with Trichoderma and carbendazim also had a significant effect on reducing disease severity. In a separate experiment, Trichoderma with and without pathogen gave significantly higher shoot length, root length, shoot weight and root weight when compared with untreated control.

Management of collar rot disease in chickpea by Trichoderma species

2020 ◽  
Vol 7 (03) ◽  
Author(s):  
PREM PANDEY ◽  
G. C. SAGAR ◽  
SUNDARMAN SHRESTHA2 ◽  
HIRAKAJI MANANDHAR ◽  
RITESH K. YADAV ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Mycelial Growth ◽  
Sclerotium Rolfsii ◽  
Maximum Growth ◽  
Pot Experiment ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Collar Rot ◽  
Rot Disease

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).

scholarly journals Chemical and biological control of Fusarium species involved in garlic dry rot at early crop stages

2021 ◽  
Author(s):  
Letizia Mondani ◽  
Giorgio Chiusa ◽  
Paola Battilani
Keyword(s):  
Growth Inhibition ◽  
Fusarium Species ◽  
Maximum Growth ◽  
Severity Index ◽  
Field Application ◽  
Dry Rot ◽  
Great Capacity ◽  
Chemical Products

AbstractThe aim of the study was to test in vitro and in vivo the efficacy of triazoles and biocontrol agents (BCAs) against Fusarium proliferatum and F. oxysporum, the former signaled as the main causal agent of garlic dry rot and the latter also involved. In vitro trials were organized using potato dextrose agar with added chemicals or BCAs inoculated with selected F. proliferatum and F. oxysporum. Garlic cloves were dipped before sowing in suspensions prepared with the fungicides showing the best performances in vitro; then they were dipped in Fusaria suspension before sowing. In in vitro trials, the maximum Fusaria growth inhibition was performed by Propiconazole + Prochloraz (100%), followed by Tebuconazole (88.9%). BCAs showed great capacity to control Fusaria, with a maximum growth inhibition of 80% (Trichoderma harzianum + T. gamsii). In vivo bacterial BCAs showed a similar capacity to control F. proliferatum and F. oxysporum compared to chemical products (mean of severity index 18.6% and 11.7%, respectively). In vivo results confirmed the in vitro performances, except for Trichoderma, which had the worst performances in vivo. Therefore, the results are preliminary but promising for future field application.

scholarly journals Alternaria leaf spot of broccoli caused by Alternaria alternata in Bangladesh

2021 ◽  
Author(s):  
Sayma T. Nira ◽  
Md. Farhad Hossain ◽  
Nur Uddin Mahmud ◽  
Oliul Hassan ◽  
Md. Tofazzal Islam ◽  
...  
Keyword(s):  
Alternaria Alternata ◽  
Leaf Spot ◽  
Maximum Growth ◽  
Pathogenicity Test ◽  
Culture Studies ◽  
Alternaria Leaf Spot ◽  
Cultural Methods ◽  
Molecular Phylogenetic ◽  
Alternaria Sp

  This study aimed to isolate and characterise the pathogen associated with Alternaria leaf spot on broccoli and to evaluate the inhibitory effects of fungicides against it. We isolated and identified the fungal pathogen as Alternaria sp. using morphological and cultural methods. Based on the aligned sequences of the internal transcribed spacer (ITS) and molecular phylogenetic analysis by the neighbour-joining method, the isolates (Ab1 and Ab2) were confirmed as Alternaria alternata. The conidia of the isolates were dark brown, cylindrical, obclavate to muriform. The conidiophores were olivaceous brown, septate, and branched. The conidial morphology of the isolates ranged from 52.4–92.4 × 10–20 μm with 2–6 transverse and 0–3 longitudinal septa. Both isolates yielded positive results in the pathogenicity test on broccoli leaves by developing brown and circular spots with concentric rings on the leaves surrounded by yellow halos. The culture studies revealed that the maximum growth of the pathogen was obtained at 30 °C and pH 6.0. Tilt 250 WC showed the highest potential in suppressing the mycelial growth of the A. alternata in vitro at a concentration as low as 50 µg/mL. The results from this study contributed to the positive identification of the pathogen and characterised A. alternata as a destructive pathogen of broccoli which may be successfully controlled by the fungicide Tilt.  

scholarly journals EVALUATION OF DIFFERENT ESSENTIAL OILS AND BIO CONTROL AGENTS AGAINST ALTERNARIA ALTERNATA THE CAUSAL AGENT OF FRUIT ROT OF JUJUBE

2020 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Keyword(s):  
Growth Inhibition ◽  
Essential Oils ◽  
Alternaria Alternata ◽  
Colony Growth ◽  
Causal Agent ◽  
Biocontrol Agents ◽  
Fruit Rot ◽  
Pathogenicity Test ◽  
Neem Oil

This study was carried out on the evaluation of different essential oils and biocontrol agents against Alternaria alternata the causal agent of fruit rot. For the pathogenicity test against A. alternata was performed through cut and injection inoculation methods. The antifungal potential of different essential oils like turpentine, laung, neem and castus root was carried out at different doses i.e. 5, 10 and 15% by food poisoned method to find out the effective and suitable oil for the growth inhibition of A. alternata and evaluate the effect of some biocontrol agents on growth inhibition of A. alternata. The findings of this investigation stated that cut method of inoculation showed higher percentage (2.60) of rotting as compared to injection method of inoculation (2.35). Minimum colony growth of A. alternata (31.60, 21.25 and 15.16%) was examined under Laung oil at the dosage of 5, 10 and 15% followed by Neem oil (42.60, 31.60 and 21.30%), respectively. Maximum colony growth of A. alternata (62.71, 52.40 and 41.75%) was observed under Castus root oil at the dosage of 5, 10 and 15%. Zero growth of target pathogen was examined under Turpentine at 5, 10 and 15%. Under control the A. alternata showed (90 mm) colony growth. Minimum linear colony growth of A. alternata was observed for Hypoxylon Sp1 (50.31%), followed by Neurospora spp. (52.97%), Lasiodiplodia theobromae (54.7%), Chactomium subaffine (57.07%) and Fusarium sp. (65.4%). Maximum mycelial colony growth (90%) was recorded in control. Based on present investigation, Similarly, for controlling the linear colony growth of A. alternata under in vitro conditions Turpentine oil ranked 1st, Laung oil ranked 2nd, Neem oil ranked 3rd, Castus root oil ranked 4th.

scholarly journals Effect of oil cake extract and organic manure on wilt of chickpea (Cicer areitinum L.) caused by Fusarium oxysporum f. sp. ciceri in vitro

2021 ◽  
Vol 17 (2) ◽  
pp. 146-148
Author(s):  
Ashish Shrivastava
Keyword(s):  
Growth Inhibition ◽  
Fusarium Oxysporum ◽  
Maximum Growth ◽  
Inhibitory Effect ◽  
Organic Manure ◽  
Percent Reduction ◽  
Organic Manures ◽  
Neem Cake ◽  
Oil Cakes

Studies were conducted to test the effect of oil cakes and organic manures on the growth of wilt pathogen under in vitro conditions. The extract of different oil cakes and organic manures were tested against F. oxysporum f. sp. ciceri by poisoned food techniques in vitro. Least growth of pathogen was recorded in extracts of Neem cake showing excellent inhibitory effect of 70.87 percent reduction over control. Next best in order of mustard cake (65.36 %), linseed (62.99%), groundnut (53.36%) and least by other. Among the organic manures tested, vermi compost and FYM showed maximum growth inhibition of 35.95 and 30.62 percent over control, respectively.

In-Vitro Efficacy of Different Fungicides Against Leaf Spot of Marigold Caused by Alternaria alternata

2021 ◽  
Vol 1 (1) ◽  
pp. 1-4
Author(s):  
Keyword(s):  
Lower Limit ◽  
Alternaria Alternata ◽  
Leaf Spot ◽  
Disease Incidence ◽  
Colony Growth ◽  
Good Effect ◽  
Upper Limit ◽  
Disease Occurrence ◽  
Chemical Fungicides

This study was carried out to study the in-vitro efficacy of different chemical fungicides leaf spot of marigold, caused by Alternaria alternata. The results of present study showed that the disease incidence from various locations of district Hyderabad Sindh was observed Ghangra Mori 35%, TandoHyder 30%, Rahooki 25%, Moosa Khatyan20%, and Hosri 15%. Upper limit disease occurrence was observed from Ghangra Mori and lower limit sickness was recorded from Hosri. The chemical fungicide Nativo observed 43%, 25.5% and 18.5% linear colony growth of Alternaria alternata at 100, 200 and 400 ppm, respectively. Aliete observed 52, 31.5 and 21 respectively. Carbandazm observed 52.5, 29.5 and 21mm respectively and Mancozeb observed 51, 28.5 and 20mm respectively. Lower limit growth of Alternaria alternata were judge in 400 ppm instead Nativo pursuited by Mancozeb, Carbandazm and Aliete, separately. In control there was 90mm was recorded. It was concluded that chemical fungicide ‘Nativo’ were full effective to stop the disperssion of Alternaria alternata in 400 ppm. Anytime the plant infusion, Neem infusion show good effect opposite linear colony growth of Alternaria alternata on 6% solution

PEPTIDE IMUNOFAN AND MOXIFLOXACIN COMBINED EFFECTS ON TOXIGENIC STRAIN CLOSTRIDIUM DIFFICILE IN VITRO

2020 ◽  
Vol 65 (8) ◽  
pp. 516-520
Author(s):  
M. G. Shvydkaya ◽  
A. M. Zatevalov ◽  
S. D. Mitrokhin ◽  
D. T. Dzhandarova ◽  
A. Yu. Mironov
Keyword(s):  
Growth Rate ◽  
Clostridium Difficile ◽  
Growth Inhibition ◽  
Maximum Growth ◽  
Combined Effects ◽  
Toxigenic Strain

Moxifloxacin and imunofan peptide concentrations - dependent Clostridium difficile growth rate was analyzed in vitro. In the course of our study, it was revealed imunofan peptide at concentrations 0.05, 0.25 μg/ml has antimicrobial characteristics against toxigenic C. difficile strain. At the same time, with the peptide and the antibiotic combined interaction, we observed moxifloxacin concentration 0-1.5 MIC stimulates C. difficile growth, regardless of the imunofan concentration. Concentrations of maximum growth inhibition for C. difficile were also established with the combined effects peptide imunofan and antibiotic moxifloxacin - 1.5 μg/ml and 2.5 MIC, respectively.

scholarly journals In Vitro Studies on the Growth Inhibiting Potential of Some Botanical Extracts Against Macrophomina phaseolina

2021 ◽  
Vol 1 (1) ◽  
pp. 15-20
Author(s):  
Asfand Iqbal ◽  
Rabia Khursid ◽  
Nida Fatima ◽  
Muhammad Rizwan
Keyword(s):  
Growth Inhibition ◽  
Plant Extracts ◽  
Allium Sativum ◽  
Standard Dose ◽  
Macrophomina Phaseolina ◽  
Colony Growth ◽  
Maximum Diameter ◽  
Highly Effective ◽  
Dose Levels

Natural plant extracts are valuable source of numerous fungitoxic compounds that can substitute synthetic fungicides. In current studies, six plant extracts viz., Zingiber officinalis, Eucalyptus camaldulensis, Azadirachta indica, Allium cepa, Cassia fistula and Allium sativum in three dose levels standard dose (S.D), S/2 and S/3 were tested against colony growth of Macrophomina phaseolina under in vitro conditions. Among all the treatments ginger extract at its standard dose was found highly effective followed by eucalyptus and neem. Percentage colony growth inhibition of (50 %), (38 %) and (29 %) was recorded to produce by ginger, eucalyptus and ginger respectively as compared to control treatment where no growth inhibition was recorded. Extract of Allium sativum was found least effective phytochemical where pathogenic fungal growth inhibition was (24.5 %). Concentration comparison of three dose levels showed that (S.D) was highly effective where mean colony growth of M. phaseolina was (3.72 cm) followed by (4 cm) @ S/2 and maximum diameter was (4.36 cm) @ S/3 concentration.

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