Faculty Opinions recommendation of AIRE recruits P-TEFb for transcriptional elongation of target genes in medullary thymic epithelial cells.

2007 ◽  
Author(s):  
Jin-Xiong She
Keyword(s):  
Epithelial Cells ◽  
Target Genes ◽  
Thymic Epithelial Cells ◽  
Transcriptional Elongation ◽  
Medullary Thymic Epithelial Cells

scholarly journals AIRE Recruits P-TEFb for Transcriptional Elongation of Target Genes in Medullary Thymic Epithelial Cells

2007 ◽  
Vol 27 (24) ◽  
pp. 8815-8823 ◽  
Author(s):  
Irena Oven ◽  
Naděžda Brdičková ◽  
Jiri Kohoutek ◽  
Tomaž Vaupotič ◽  
Mojca Narat ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Target Genes ◽  
Ectopic Expression ◽  
Lymphocytic Infiltration ◽  
Salivary Protein ◽  
Thymic Epithelial Cells ◽  
Transcriptional Elongation ◽  
Medullary Thymic Epithelial Cells

ABSTRACT AIRE is a transcriptional activator that directs the ectopic expression of many tissue-specific genes in medullary thymic epithelial cells, which plays an important role in the negative selection of autoreactive T cells. However, its mechanism of action remains poorly understood. In this study, we found that AIRE regulates the step of elongation rather than initiation of RNA polymerase II. For these effects, AIRE bound and recruited P-TEFb to target promoters in medullary thymic epithelial cells. In these cells, AIRE activated the ectopic transcription of insulin and salivary protein 1 genes. Indeed, by chromatin immunoprecipitation, we found that RNA polymerase II was already engaged on these promoters but was unable to elongate in the absence of AIRE. Moreover, the genetic inactivation of cyclin T1 from P-TEFb abolished the transcription of AIRE-responsive genes and led to lymphocytic infiltration of lacrimal and salivary glands in the CycT1−/− mouse. Our findings reveal critical steps by which AIRE regulates the transcription of genes that control central tolerance in the thymus.

scholarly journals Lymphotoxin β Receptor Regulates the Development of CCL21-Expressing Subset of Postnatal Medullary Thymic Epithelial Cells

2013 ◽  
Vol 190 (10) ◽  
pp. 5110-5117 ◽  
Author(s):  
Enkhsaikhan Lkhagvasuren ◽  
Mie Sakata ◽  
Izumi Ohigashi ◽  
Yousuke Takahama
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Medullary Thymic Epithelial Cells ◽  
Β Receptor

scholarly journals Foxn1 Regulates Lineage Progression in Cortical and Medullary Thymic Epithelial Cells But Is Dispensable for Medullary Sublineage Divergence

PLoS Genetics ◽  
2011 ◽  
Vol 7 (11) ◽  
pp. e1002348 ◽  
Author(s):  
Craig S. Nowell ◽  
Nicholas Bredenkamp ◽  
Stéphanie Tetélin ◽  
Xin Jin ◽  
Christin Tischner ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Medullary Thymic Epithelial Cells

scholarly journals Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Beth Lucas ◽  
Andrea J. White ◽  
Emilie J. Cosway ◽  
Sonia M. Parnell ◽  
Kieran D. James ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Inkt Cells ◽  
Medullary Thymic Epithelial Cells

Ultrastructure of medullary thymic epithelial cells of autoimmune regulator (Aire)‐deficient mice

2009 ◽  
Vol 88 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Živana Milićević ◽  
Novica M Milićević ◽  
Martti Laan ◽  
Pärt Peterson ◽  
Kai Kisand ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Autoimmune Regulator ◽  
Medullary Thymic Epithelial Cells ◽  
Deficient Mice

scholarly journals NF-κB2 Is Required for the Control of Autoimmunity by Regulating the Development of Medullary Thymic Epithelial Cells

2006 ◽  
Vol 281 (50) ◽  
pp. 38617-38624 ◽  
Author(s):  
Baochun Zhang ◽  
Zhe Wang ◽  
Jane Ding ◽  
Pärt Peterson ◽  
William T. Gunning ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Medullary Thymic Epithelial Cells
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