Faculty Opinions recommendation of Zonula occludens-1 and -2 regulate apical cell structure and the zonula adherens cytoskeleton in polarized epithelia.

2012 ◽  
Author(s):  
Kris DeMali ◽  
Jessica Maiers
Keyword(s):  
Cell Structure ◽  
Apical Cell ◽  
Zonula Occludens ◽  
Polarized Epithelia ◽  
Zonula Occludens 1

scholarly journals Zonula occludens-1 and -2 regulate apical cell structure and the zonula adherens cytoskeleton in polarized epithelia

2012 ◽  
Vol 23 (4) ◽  
pp. 577-590 ◽  
Author(s):  
Alan S. Fanning ◽  
Christina M. Van Itallie ◽  
James M. Anderson
Keyword(s):  
Actin Cytoskeleton ◽  
Structural Changes ◽  
Cell Structure ◽  
Apical Cell ◽  
Rho Kinase ◽  
Cytoskeletal Proteins ◽  
Cell Junctions ◽  
Cortical Actin ◽  
Zonula Occludens ◽  
And Function

The structure and function of both adherens (AJ) and tight (TJ) junctions are dependent on the cortical actin cytoskeleton. The zonula occludens (ZO)-1 and -2 proteins have context-dependent interactions with both junction types and bind directly to F-actin and other cytoskeletal proteins, suggesting ZO-1 and -2 might regulate cytoskeletal activity at cell junctions. To address this hypothesis, we generated stable Madin-Darby canine kidney cell lines depleted of both ZO-1 and -2. Both paracellular permeability and the localization of TJ proteins are disrupted in ZO-1/-2–depleted cells. In addition, immunocytochemistry and electron microscopy revealed a significant expansion of the perijunctional actomyosin ring associated with the AJ. These structural changes are accompanied by a recruitment of 1-phosphomyosin light chain and Rho kinase 1, contraction of the actomyosin ring, and expansion of the apical domain. Despite these changes in the apical cytoskeleton, there are no detectable changes in cell polarity, localization of AJ proteins, or the organization of the basal and lateral actin cytoskeleton. We conclude that ZO proteins are required not only for TJ assembly but also for regulating the organization and functional activity of the apical cytoskeleton, particularly the perijunctional actomyosin ring, and we speculate that these activities are relevant both to cellular organization and epithelial morphogenesis.

Zonula occludens-1 demonstrates a unique appearance in buccal mucosa over several layers

2021 ◽  
Author(s):  
Keisuke Imafuku ◽  
Mayumi Kamaguchi ◽  
Ken Natsuga ◽  
Hideki Nakamura ◽  
Hiroshi Shimizu ◽  
...  
Keyword(s):  
Buccal Mucosa ◽  
Zonula Occludens ◽  
Zonula Occludens 1

scholarly journals PATH-04. THE BLOOD-BRAIN BARRIER IN DIFFUSE MIDLINE GLIOMA AND ITS IMPLICATIONS FOR DRUG DELIVERY

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii164-ii164
Author(s):  
Rianne Haumann ◽  
Fatma El-Khouly ◽  
Marjolein Breur ◽  
Sophie Veldhuijzen van Zanten ◽  
Gertjan Kaspers ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Blood Vessels ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Vascular Density ◽  
Zonula Occludens ◽  
Blood Brain ◽  
Zonula Occludens 1 ◽  
End Stage ◽  
Diffuse Midline Glioma

Abstract INTRODUCTION Chemotherapy has been unsuccessful for pediatric diffuse midline glioma (DMG) most likely due to an intact blood-brain barrier (BBB). However, the BBB has not been characterized in DMG and therefore its implications for drug delivery are unknown. In this study we characterized the BBB in DMG patients and compared this to healthy controls. METHODS End-stage DMG pontine samples (n=5) were obtained from the VUmc diffuse intrinsic pontine glioma (DIPG) autopsy study and age-matched healthy pontine samples (n=22) were obtained from the NIH NeuroBioBank. Tissues were stained for BBB markers claudin-5, zonula occludens-1, laminin, and PDGFRβ. Claudin-5 stains were used to determine vascular density and diameter. RESULTS In DMG, expression of claudin-5 was reduced and dislocated to the abluminal side of endothelial cells. In addition, the expression of zonula occludens-1 was reduced. The basement membrane protein laminin expression was reduced at the glia limitans in both pre-existent vessels and neovascular proliferation. PDGFRβ expression was not observed in DMG but was present in healthy pons. Furthermore, the number of blood vessels in DMG was significantly (P< 0.01) reduced (13.9 ± 11.8/mm2) compared to healthy pons (26.3 ± 14.2/mm2). Markedly, the number of small blood vessels (< 10µm) was significantly lower (P< 0.01) while larger blood vessels (> 10µm) were not significantly different (P= 0.223). The mean vascular diameter was larger for DMG 9.3 ± 9.9µm compared to 7.7 ± 9.0µm for healthy pons (P= 0.016). CONCLUSION Both the BBB and the vasculature are altered at end-stage DMG. The reduced vascular density might have implications for several drug delivery methods such as focused ultrasound and convection enhanced delivery that are being explored for the treatment of DMG. The functional effects of the structurally altered BBB remain unknown and further research is needed to evaluate the BBB integrity at end-stage DMG

scholarly journals Effects of Interleukin-13 and Montelukast on the Expression of Zonula Occludens-1 in Human Podocytes

2015 ◽  
Vol 56 (2) ◽  
pp. 426 ◽  
Author(s):  
Se Jin Park ◽  
Moin A. Saleem ◽  
Ja-Ae Nam ◽  
Tae-Sun Ha ◽  
Jae Il Shin
Keyword(s):  
Interleukin 13 ◽  
Zonula Occludens ◽  
Zonula Occludens 1

scholarly journals Vinculin directly binds zonula occludens-1 and is essential for stabilizing connexin-43-containing gap junctions in cardiac myocytes

2014 ◽  
Vol 127 (5) ◽  
pp. 1104-1116 ◽  
Author(s):  
A. E. Zemljic-Harpf ◽  
J. C. Godoy ◽  
O. Platoshyn ◽  
E. K. Asfaw ◽  
A. R. Busija ◽  
...  
Keyword(s):  
Gap Junctions ◽  
Cardiac Myocytes ◽  
Connexin 43 ◽  
Zonula Occludens ◽  
Zonula Occludens 1

scholarly journals Decreased ZO1 expression causes loss of time-dependent tight junction function in the liver of ob/ob mice

2021 ◽  
Author(s):  
Yuya Tsurudome ◽  
Nao Morita ◽  
Michiko Horiguchi ◽  
Kentaro Ushijima
Keyword(s):  
Tight Junction ◽  
Dark Period ◽  
Vital Role ◽  
Time Dependent ◽  
Signal Transduction System ◽  
Binding Ability ◽  
Protein Levels ◽  
Multiple Organs ◽  
Zonula Occludens ◽  
Zonula Occludens 1

Abstract Diabetes patients are at a high risk of developing complications related to angiopathy and disruption of the signal transduction system. The liver is one of the multiple organs damaged during diabetes. Few studies have evaluated the morphological effects of adhesion factors in diabetic liver. The influence of diurnal variation has been observed in the expression and functioning of adhesion molecules to maintain tissue homeostasis associated with nutrient uptake. The present study demonstrated that the rhythm-influenced functioning of tight junction was impaired in the liver of ob/ob mice. The tight junctions of hepatocytes were loosened during the dark period in normal mice compared to those in ob/ob mice, where the hepatocyte gaps remained open throughout the day. The time-dependent expression of zonula occludens 1 (ZO1) in the liver plays a vital role in the functioning of the tight junction. The time-dependent expression of ZO1 was nullified and its expression was attenuated in the liver of ob/ob mice. ZO1 expression was inhibited at the mRNA and protein levels. The expression rhythm of ZO1 was found to be regulated by heat shock factor (HSF)1/2, the expression of which was reduced in the liver of ob/ob mice. The DNA-binding ability of HSF1/2 was decreased in the liver of ob/ob mice compared to that in normal mice. These findings suggest the involvement of impaired expression and functioning of adhesion factors in diabetic liver complications.

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