ABSTRACTLegionella pneumophilagenes encoding LapA, LapB, and PlaC were identified as the most highly upregulated type II secretion (T2S) genes during infection ofAcanthamoeba castellanii, although these genes had been considered dispensable on the basis of the behavior of mutants lacking eitherlapAandlapBorplaC. AplaCmutant showed even higher levels oflapAandlapBtranscripts, and alapA lapBmutant showed heightening ofplaCmRNA levels, suggesting that the role of the LapA/B aminopeptidase is compensatory with respect to that of the PlaC acyltransferase. Hence, we made double mutants and found thatlapA plaCmutants have an ~50-fold defect during infection ofA. castellanii. These data revealed, for the first time, the importance of LapA in any sort of infection; thus, we purified LapA and defined its crystal structure, activation by another T2S-dependent protease (ProA), and broad substrate specificity. When the amoebal infection medium was supplemented with amino acids, the defect of thelapA plaCmutant was reversed, implying that LapA generates amino acids for nutrition. Since the LapA and PlaC data did not fully explain the role of T2S in infection, we identified, via proteomic analysis, a novel secreted protein (NttD) that promotes infection ofA. castellanii. AlapA plaC nttDmutant displayed an even greater (100-fold) defect, demonstrating that the LapA, PlaC, and NttD data explain, to a significant degree, the importance of T2S. LapA-, PlaC-, and NttD-like proteins had distinct distribution patterns within and outside theLegionellagenus. LapA was notable for having as its closest homologue anA. castellaniiprotein.IMPORTANCETransmission ofL. pneumophilato humans is facilitated by its ability to grow inAcanthamoebaspecies. We previously documented that type II secretion (T2S) promotesL. pneumophilainfection ofA. castellanii. Utilizing transcriptional analysis and proteomics, double and triple mutants, and crystal structures, we defined three secreted substrates/effectors that largely clarify the role of T2S during infection ofA. castellanii. Particularly interesting are the unique functional overlap between an acyltransferase (PlaC) and aminopeptidase (LapA), the broad substrate specificity and eukaryotic-protein-like character of LapA, and the novelty of NttD. Linking LapA to amino acid acquisition, we defined, for the first time, the importance of secreted aminopeptidases in intracellular infection. Bioinformatic investigation, not previously applied to T2S, revealed that effectors originate from diverse sources and distribute within theLegionellagenus in unique ways. The results of this study represent a major advance in understandingLegionellaecology and pathogenesis, bacterial secretion, and the evolution of intracellular parasitism.