Faculty Opinions recommendation of Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.

Abstract Motivation Transcription factors (TFs) are key regulators of gene expression, and can activate or repress multiple target genes, forming regulatory units, or regulons. Understanding downstream effects of these regulators includes evaluating how TFs cooperate or compete within regulatory networks. Here we present RTNduals, an R/Bioconductor package that implements a general method for analyzing pairs of regulons. Results RTNduals identifies a dual regulon when the number of targets shared between a pair of regulators is statistically significant. The package extends the RTN (Reconstruction of Transcriptional Networks) package, and uses RTN transcriptional networks to identify significant co-regulatory associations between regulons. The Supplementary Information reports two case studies for TFs using the METABRIC and TCGA breast cancer cohorts. Availability and implementation RTNduals is written in the R language, and is available from the Bioconductor project at http://bioconductor.org/packages/RTNduals/. Supplementary information Supplementary data are available at Bioinformatics online.

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Mutational Analyses of Multiple Target Genes in Histologically Heterogeneous Gastric Cancer with Microsatellite Instability

Japanese Journal of Cancer Research ◽

10.1111/j.1349-7006.1998.tb00525.x ◽

1998 ◽

Vol 89 (12) ◽

pp. 1284-1291 ◽

Cited By ~ 12

Author(s):

Ying Wang ◽

Kazuya Shinmura ◽

Rong-Jun Guo ◽

Jun Isogaki ◽

Dong-Yu Wang ◽

...

Keyword(s):

Gastric Cancer ◽

Microsatellite Instability ◽

Target Genes ◽

Multiple Target

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Systems Pharmacological Approach to the Effect of Bulsu-san Promoting Parturition

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/7236436 ◽

2017 ◽

Vol 2017 ◽

pp. 1-15 ◽

Cited By ~ 5

Author(s):

Su Yeon Suh ◽

Won G. An

Keyword(s):

Analytical Approach ◽

Target Genes ◽

Clinical Applications ◽

Oriental Medicine ◽

Biological Processes ◽

Gene Ontology Analysis ◽

Multiple Target ◽

Pathway Network ◽

Target Network ◽

Compound Target

Bulsu-san (BSS) has been commonly used in oriental medicine for pregnant women in East Asia. The purpose of this research was to elucidate the effect of BSS on ease of parturition using a systems-level in silico analytic approach. Research results show that BSS is highly connected to the parturition related pathways, biological processes, and organs. There were numerous interactions between most compounds of BSS and multiple target genes, and this was confirmed using herb-compound-target network, target-pathway network, and gene ontology analysis. Furthermore, the mRNA expression of relevant target genes of BSS was elevated significantly in related organ tissues, such as those of the uterus, placenta, fetus, hypothalamus, and pituitary gland. This study used a network analytical approach to demonstrate that Bulsu-san (BSS) is closely related to the parturition related pathways, biological processes, and organs. It is meaningful that this systems-level network analysis result strengthens the basis of clinical applications of BSS on ease of parturition.

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Protein kinase Msk1 physically and functionally interacts with the KMT2A/MLL1 methyltransferase complex and contributes to the regulation of multiple target genes

Epigenetics & Chromatin ◽

10.1186/s13072-016-0103-3 ◽

2016 ◽

Vol 9 (1) ◽

Cited By ~ 15

Author(s):

Maaike Wiersma ◽

Marianne Bussiere ◽

John A. Halsall ◽

Nil Turan ◽

Robert Slany ◽

...

Keyword(s):

Protein Kinase ◽

Target Genes ◽

Multiple Target

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The MicroRNA Family Both in Normal Development and in Different Diseases: The miR-17-92 Cluster

BioMed Research International ◽

10.1155/2019/9450240 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 8

Author(s):

Xiaodan Bai ◽

Shengyu Hua ◽

Junping Zhang ◽

Shixin Xu

Keyword(s):

Small Molecules ◽

Target Genes ◽

Normal Development ◽

Neurological Diseases ◽

Multiple Target ◽

Cellular Processes ◽

Microrna Family ◽

Mirna Clusters ◽

And Function

An increasing number of research studies over recent years have focused on the function of microRNA (miRNA) molecules which have unique characteristics in terms of structure and function. They represent a class of endogenous noncoding single-strand small molecules. An abundance of miRNA clusters has been found in the genomes of various organisms often located in a polycistron. The miR-17-92 family is among the most famous miRNAs and has been identified as an oncogene. The functions of this cluster, together with the seven individual molecules that it comprises, are most related to cancers, so it would not be surprising that they are considered to have involvement in the development of tumors. The miR-17-92 cluster is therefore expected not only to be a tumor marker, but also to perform an important role in the early diagnosis of those diseases and possibly also be a target for tumor biotherapy. The miR-17-92 cluster affects the development of disease by regulating many related cellular processes and multiple target genes. Interestingly, it also has important roles that cannot be ignored in disease of the nervous system and circulation and modulates the growth and development of bone. Therefore, it provides new opportunities for disease prevention, clinical diagnosis, prognosis, and targeted therapy. Here we review the role of the miR-17-92 cluster that has received little attention in relation to neurological diseases, cardiac diseases, and the development of bone and tumors.

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