Faculty Opinions recommendation of Identification of Root-Secreted Compounds Involved in the Communication Between Cucumber, the Beneficial Bacillus amyloliquefaciens, and the Soil-Borne Pathogen Fusarium oxysporum.

2017 ◽  
Author(s):  
Ben Lugtenberg
Keyword(s):  
Fusarium Oxysporum ◽  
Bacillus Amyloliquefaciens

scholarly journals Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7

2017 ◽  
Vol 35 (No. 2) ◽  
pp. 113-121
Author(s):  
Wang Junhua ◽  
Zhao Shuangzhi ◽  
Qiu Jiying ◽  
Zhou Qingxin ◽  
Li Xiaoyong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Molecular Mass ◽  
Fusarium Oxysporum ◽  
Bacillus Amyloliquefaciens ◽  
Fungicidal Activity ◽  
Pathogenic Fungi ◽  
Antifungal Activities ◽  
Minimum Fungicidal Concentration ◽  
Antimicrobial Substances ◽  
Wide Range

Bacillus amyloliquefaciens NCPSJ7 could secrete extracellular antimicrobial substances, showing potent antifungal activities. An active peptide AFP3 was isolated from the fermentation supernatant. After chromatography, the purified peptide was tested for the fungicidal activity, molecular mass, and stability. The results indicated that the peptide with a molecular mass of around 3.3 kDa, showed discernible inhibition of the pathogen Fusarium oxysporum f. sp. niveum with the minimum fungicidal concentration of 31 µg/ml. It also exhibited excellent inhibition of some representative pathogenic fungi at a low concentration. Moreover, the peptide remained active at a wide range of temperatures and pH. Ion Na<sup>+</sup> may even increase the antifungal activities. At the same time, the peptide could well tolerate the treatment with trypsin. Electron microscopy was used to investigate the effect of the peptide on the pathogens. The peptide inhibited the growth of pathogens by disrupting the integrity of the hyphal membranes, resulting in their lysis. The potent fungicidal activities and stability made the peptide be a candidate for a biopreservative.

scholarly journals Identification of Root-Secreted Compounds Involved in the Communication Between Cucumber, the Beneficial Bacillus amyloliquefaciens, and the Soil-Borne Pathogen Fusarium oxysporum

2017 ◽  
Vol 30 (1) ◽  
pp. 53-62 ◽  
Author(s):  
Yunpeng Liu ◽  
Lin Chen ◽  
Gengwei Wu ◽  
Haichao Feng ◽  
Guishan Zhang ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Root System ◽  
Root Exudates ◽  
Bacillus Amyloliquefaciens ◽  
Root Colonization ◽  
Plant Root ◽  
Root Exudation ◽  
Soil Borne Pathogens ◽  
Split Root System ◽  
Cucumber Roots

Colonization of plant growth–promoting rhizobacteria (PGPR) is critical for exerting their beneficial effects on the plant. Root exudation is a major factor influencing the colonization of both PGPR and soil-borne pathogens within the root system. However, the tripartite interaction of PGPR, plant roots, and soil-borne pathogens is poorly understood. We screened root exudates for signals that mediate tripartite interactions in the rhizosphere. In a split-root system, we found that root colonization of PGPR strain Bacillus amyloliquefaciens SQR9 on cucumber root was significantly enhanced by preinoculation with SQR9 or the soil-borne pathogen Fusarium oxysporum f. sp. cucumerinum, whereas root colonization of F. oxysporum f. sp. cucumerinum was reduced upon preinoculation with SQR9 or F. oxysporum f. sp. cucumerinum. Root exudates from cucumbers preinoculated with SQR9 or F. oxysporum f. sp. cucumerinum were analyzed and 109 compounds were identified. Correlation analysis highlighted eight compounds that significantly correlated with root colonization of SQR9 or F. oxysporum f. sp. cucumerinum. After performing colonization experiments with these chemicals, raffinose and tryptophan were shown to positively affect the root colonization of F. oxysporum f. sp. cucumerinum and SQR9, respectively. These results indicate that cucumber roots colonized by F. oxysporum f. sp. cucumerinum or SQR9 increase root secretion of tryptophan to strengthen further colonization of SQR9. In contrast, these colonized cucumber roots reduce raffinose secretion to inhibit root colonization of F. oxysporum f. sp. cucumerinum.

scholarly journals An Endophytic Strain of Bacillus amyloliquefaciens Suppresses Fusarium oxysporum Infection of Chinese Wolfberry by Altering Its Rhizosphere Bacterial Community

2022 ◽  
Vol 12 ◽  
Author(s):  
Constantine Uwaremwe ◽  
Liang Yue ◽  
Yun Wang ◽  
Yuan Tian ◽  
Xia Zhao ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Fusarium Oxysporum ◽  
Root Rot ◽  
Bacillus Amyloliquefaciens ◽  
Fungal Pathogens ◽  
Metagenomic Sequencing ◽  
Lycium Barbarum ◽  
Root Rot Disease ◽  
Rot Disease ◽  
Biocontrol Strain

Root rot disease is a serious infection leading to production loss of Chinese wolfberry (Lycium barbarum). This study tested the potential for two bacterial biological control agents, Bacillus amyloliquefaciens HSB1 and FZB42, against five fungal pathogens that frequently cause root rot in Chinese wolfberry. Both HSB1 and FZB42 were found to inhibit fungal mycelial growth, in vitro and in planta, as well as to promote the growth of wolfberry seedlings. In fact, a biocontrol experiment showed efficiency of 100% with at least one treatment involving each biocontrol strain against Fusarium oxysporum. Metagenomic sequencing was used to assess bacterial community shifts in the wolfberry rhizosphere upon introduction of each biocontrol strain. Results showed that HSB1 and FZB42 differentially altered the abundances of different taxa present and positively influenced various functions of inherent wolfberry rhizosphere bacteria. This study highlights the application of biocontrol method in the suppression of fungal pathogens that cause root rot disease in wolfberry, which is useful for agricultural extension agents and commercial growers.

Multifaceted benefits of Bacillus amyloliquefaciens strain FBZ24 in the management of wilt disease in tomato caused by Fusarium oxysporum f. sp. lycopersici

2018 ◽  
Vol 103 ◽  
pp. 92-101 ◽  
Author(s):  
K. Elanchezhiyan ◽  
U. Keerthana ◽  
K. Nagendran ◽  
S.R. Prabhukarthikeyan ◽  
K. Prabakar ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Bacillus Amyloliquefaciens ◽  
Wilt Disease

scholarly journals Antifungal Activity of Bacillus amyloliquefaciens NJN-6 Volatile Compounds against Fusarium oxysporum f. sp. cubense

2012 ◽  
Vol 78 (16) ◽  
pp. 5942-5944 ◽  
Author(s):  
Jun Yuan ◽  
Waseem Raza ◽  
Qirong Shen ◽  
Qiwei Huang
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Volatile Compounds ◽  
Spore Germination ◽  
Bacillus Amyloliquefaciens ◽  
Fungal Growth ◽  
Content Type

ABSTRACTBacillus amyloliquefaciensNJN-6 produces volatile compounds (VOCs) that inhibit the growth and spore germination ofFusarium oxysporumf. sp.cubense. Among the total of 36 volatile compounds detected, 11 compounds completely inhibited fungal growth. The antifungal activity of these compounds suggested that VOCs can play important roles over short and long distances in the suppression ofFusarium oxysporum.

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