Faculty Opinions recommendation of Charge shielding prevents aggregation of supercharged GFP variants at high protein concentration.

Two routes by which interstitial pulmonary edema liquid may leave the lung during recovery are reabsorption into the pulmonary circulation and clearance by lung lymphatics. We hypothesized that reabsorption of edema liquid of low protein concentration into the pulmonary circulation would be greater than reabsorption of edema liquid of high protein concentration because of the greater protein osmotic gradient in the former. On the basis of previous studies, lymph flow should contribute minimally to the recovery. In 22 in situ perfused sheep lungs with lymph fistulas, we produced approximately 100 g of osmotic or hydrostatic edema (low protein) or increased leakiness edema by calcium depletion (high protein). To induce reabsorption, we changed the perfusate from low- (1% albumin, osmotic pressure = 4 cmH2O) to high-protein (7% albumin, osmotic pressure = 22 cmH2O) solution in the osmotic group, decreased capillary pressure from 29 +/- 9 to 11 +/- 6 cmH2O in the hydrostatic group, or reversed leakiness by adding CaCl2 to the perfusate in the increased leakiness group. Reabsorption occurred only during recovery from osmotic (40 +/- 22% of filtered liquid) and hydrostatic (15 +/- 11%) edema. Total lung lymph flow during recovery from osmotic, hydrostatic, or increased leakiness edema was 4.9 +/- 3.4, 4.3 +/- 3.4, or 3.5 +/- 1.9 g, respectively. We conclude that during recovery from pulmonary edema interstitial liquid is reabsorbed into the circulation in inverse proportion to its protein concentration. We confirm that only a small fraction of the interstitial edema liquid is cleared by the lymphatics during recovery from any type of edema.

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Optimized procedure for renaturation of recombinant human bone morphogenetic protein-2 at high protein concentration

Biotechnology and Bioengineering ◽

10.1002/bit.10906 ◽

2004 ◽

Vol 85 (6) ◽

pp. 601-609 ◽

Cited By ~ 66

Author(s):

Luis Felipe Vallejo ◽

Ursula Rinas

Keyword(s):

Bone Morphogenetic Protein ◽

Protein Concentration ◽

High Protein ◽

Human Bone ◽

Bone Morphogenetic Protein 2 ◽

Morphogenetic Protein ◽

High Protein Concentration ◽

Human Bone Morphogenetic Protein ◽

Recombinant Human Bone

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Studies on colostral and milk whey proteins in the sow 2. The effect of delayed suckling on colostrum and milk whey proteins

Animal Science ◽

10.1017/s0003356100026982 ◽

1969 ◽

Vol 11 (3) ◽

pp. 345-349 ◽

Cited By ~ 4

Author(s):

F. J. Bourne

Keyword(s):

Whey Protein ◽

Protein Concentration ◽

Whey Proteins ◽

High Protein ◽

Milk Whey ◽

Protein Levels ◽

High Protein Concentration

SUMMARYColostral whey protein levels do not change during the first 4 hr from the start of parturition when suckling is prevented. Both early and late-born piglets returned to the sow at the end of this period are able to suck colostrum of a high protein concentration. Removal of piglets from the sow and the presence of an attendant did not affect the farrowing process.

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Effects of substitution of tricaproin for tallow and of protein concentration in milk substitutes on nitrogen and energy balance in the preruminant lamb

British Journal Of Nutrition ◽

10.1079/bjn19880125 ◽

1988 ◽

Vol 60 (3) ◽

pp. 525-538

Author(s):

Bernard Aurousseau

Keyword(s):

Milk Fat ◽

Protein Concentration ◽

Coconut Oil ◽

Metabolizable Energy ◽

High Protein ◽

N Balance ◽

Total N ◽

Protein Requirements ◽

High Protein Concentration ◽

Milk Substitute

1. Results of 138 nitrogen balance studies from experiments with forty male Limousin and nineteen male Ile de France preruminant lambs were used to estimate total N requirements and to assess the effects of dietary tricaproin inclusion, protein concentration, lysine and methionine supplementation and the age of lambs on protein retention. In addition, energy balances were obtained in twenty-four of the Limousin lambs from birth up to 3 weeks of age by means of a slaughter technique.2. For milk substitute unsupplemented with amino acids, tricaproin inclusion increased N balance slightly (P < 0.001) when a medium-protein concentration (260 g/kg dry matter (DM)) was used, in 2-week-old lambs (+4.3%) and in 5-week-old Limousin lambs (+5.3%), or very markedly when a high-protein concentration (320 g/kg DM) was used, in 2-week-old lambs (+14.5%) and in 5-week-old lambs (+18.6%). Protein requirements decreased from 300 g/kg DM in 3-week-old lambs to 270 g/kg DM in 5-week-old lambs for the control milk containing tallow-coconut oil mixture (0.67:0.33 by wt) and was increased above 300 g/kg DM at all ages in the case of experimental milk containing tallow-coconut oil-tricaproin mixture (0.33:0.33:0.33, by wt).3. For milk containing either a medium- or a high-protein concentration and supplemented with lysine and methionine, inclusion of tricaproin increased N balance (P < 0.01) to a similar extent (+8.5 up to +14.3 %) in 1 to 2- and 3-week-old Limousin lambs. An increase in N concentration in the milk had no effect in 1 - and 2-week-old lambs, but led to a decreased N balance in 3-week-old lambs. Protein requirements decreased from 310 g/kg DM in 1-week-old lambs to 190 g/kg DM in 3-week-old lambs for the milk containing tallow-coconut oil or from 300 g/kg DM in 2-week-old lambs to 210 g/kg DM in 3-week-old lambs for the milk containing tallow-coconut oil-tricaproin.4. For the Ile de France lambs given milk supplemented with lysine and methionine, tricaproin inclusion did not alter N balance. Protein requirements decreased from 370 g/kg DM in 2-week-old lambs to 270 g/kg DM in 4-week-old lambs for milk containing both kinds of fat mixtures.5. Between birth and 3 weeks of age, efficiency of metabolizable energy (ME) utilization in the Limousin lambs was not dependent on the nature of the milk fat or the protein concentration of the diet (ME efficiency for tissue deposition varying from 0.672 to 0.698) whilst ME efficiency for protein deposition was 0.52 and ME efficiency for lipid deposition was close to 1; daily maintenance expenditure was 553 kJ/kg body-weight0.75

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Unilateral subdural-peritoneal shunting for bilateral chronic subdural hematomas in infancy

Journal of Neurosurgery ◽

10.3171/jns.1985.63.1.0134 ◽

1985 ◽

Vol 63 (1) ◽

pp. 134-137 ◽

Cited By ~ 22

Author(s):

Nobuhiko Aoki ◽

Hiroshi Mizutani ◽

Hideaki Masuzawa

Keyword(s):

Protein Concentration ◽

Low Pressure ◽

High Protein ◽

Content Type ◽

Shunt Tube ◽

Subdural Hematomas ◽

High Protein Concentration ◽

Fine Print

✓ The authors present the cases of three infants with bilateral chronic subdural hematomas (SDH's) (effusions). Communication between the hematoma cavities was confirmed by an injection of air or metrizamide into one of the cavities. In all three cases, both SDH's (effusions) were successfully treated by placement of a unilateral subdural-peritoneal shunt without any untoward consequences. It is stressed that the cavities of bilateral chronic SDH's (effusions) may communicate, and that in such cases unilateral subdural-peritoneal shunting is sufficient to eradicate the SDH's on both sides. In addition, subdural fluid, even with a high protein concentration, may be successfully eliminated by an internal shunt using a shunt tube with a large internal caliber and a low-pressure valve.

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