Avaliação da capacidade inibitória de ruta graveolens sobre candida albicans / Evaluation of the inhibitory capacity of ruta graveolens on candida albicans

The problem of drug resistance in terms of antifungal therapy, unknown until a few years ago, is assuming increasing importance. Particularly in immunosuppressed patients and subject to chemotherapy and radiotherapy. In the last years the use of essential oils as approach to improving the effectiveness of antifungal agents and reducing the antibiotic resistant has been proposed. Our research aimed to evaluate the antifungal activity of Colombian essential oil of Ruta graveolens (REO) against clinical strains of Candida albicans, Candida parapsilopsis, C. glabrata and Candida tropicalis. The data obtained showed that Candida tropicalis and Candida albicans were most sensible strains showing minimum inhibitory concentrations (MIC) of 0.5 and 1.0 µg/ml of REO. The Time Kill Kinetics assay demonstrated that REO showed fungicide effect against C. tropicalis and fungistatic effect against C. albicans. In addition, the 40% of the biofilm formed by C. albicans was eradicated using 1% of REO after 1 hour of exposure.

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Ruta graveolens, Pelargonium graveolens E Hibiscus cannabinus COMO INIBIDORES NATURAIS DO CRESCIMENTO DE Candida albicans

Revista UNINGÁ ◽

10.46311/2318-0579.58.euj4124 ◽

2021 ◽

Vol 58 (1) ◽

pp. eUJ4124

Author(s):

Camila Mara dos Reis ◽

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Letícia Maria Soares Azevedo ◽

Marisa Cristina da Fonseca Casteluber ◽

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...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Conventional Medicine ◽

Hibiscus Cannabinus ◽

In Vivo Studies ◽

Diffusion Method ◽

Ruta Graveolens ◽

Simultaneous Treatment ◽

Pelargonium Graveolens

Candidiasis is an infection caused by fungi of the genus Candida, Candida albicans being the species that most causes the disease in Brazil. The Fluconazole is the conventional medicine used as a treatment of these infections; however, it causes many adverse reactions in the patient and its continued use can induce the resistance of the pathogen. The medicinal plants can be very effective as an alternative treatment of diseases and have been used in folk medicine for years. In this study, it was evaluated the antifungal activity of extracts of Ruta graveolens, Pelargonium graveolens and Hibiscus cannabinus against the fungal development of Candida albicans, comparing the efficiency of these extracts to Fluconazole. The extracts were prepared in the concentration of 1g of the plant for each 5 mL of alcohol 70%. The data were obtained using the agar diffusion method. The minimum inhibitory concentration test (MIC) was performed, and showed that R. graveolens was able of inhibiting 100% of the pathogen when using 100 mg.ml-1 concentration. From the results obtained, it was possible to conclude that R. graveolens was the most effective extract when compared to Fluconazole, suggesting that this extract can be used as an alternative to conventional treatment to improve the efficiency of current treatments. The extracts of P. graveolens and H. cannabinus also presented antifungal activity but in smaller proportion than Fluconazole. The data from this study suggests that R. graveolens extract can be tested in future in vivo studies with the objective of proposing its use in alternative or simultaneous treatment of the synthetic drug used.

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Atividade antimicrobiana in vitro de produtos vegetais em otite externa aguda

Revista Brasileira de Otorrinolaringologia ◽

10.1590/s0034-72992008000100019 ◽

2008 ◽

Vol 74 (1) ◽

pp. 118-124 ◽

Cited By ~ 10

Author(s):

Janaina Cândida Rodrigues Nogueira ◽

Margareth de Fátima Melo Diniz ◽

Edeltrudes O. Lima

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Candida Krusei ◽

Ruta Graveolens ◽

Cymbopogon Citratus ◽

Cultura Popular ◽

Plectranthus Amboinicus

Otite externa aguda é a inflamação do conduto auditivo externo, e plantas medicinais podem ser utilizadas, na cultura popular, para seu tratamento. OBJETIVO: Avaliar atividade antimicrobiana in vitro de Aleolanthus suaveolens, Caryophyllus aromaticus, Cymbopogon citratus, Matricaria chamomila, Pithecellobium avaremotemo, Plectranthus amboinicus e Ruta graveolens sobre agentes etiológicos de otite externa. CASUÍSTICA E MÉTODOS: A concentração inibitória mínima de extratos e óleos destas plantas foi obtida em amostras de otite externa. RESULTADOS: Staphylococcus aureus em 10 culturas, Pseudomonas aeruginosa em 8, Pseudomonas aeruginosa e Staphylococcus aureus, em associação, em 5 culturas e Candida albicans e Candida krusei em 4 culturas. P. aeruginosa foi resistente a todos os extratos e óleos essenciais testados; os extratos de A. suaveolens, P. avaremotemo e de R. graveolens foram inativos, o óleo essencial de C. aromaticus e M. chamomila foram ativos contra 3 cepas de S. aureus e as cepas de Candida; Sete das cepas de S. aureus foram sensíveis ao extrato de P. amboinicus, mas o óleo não mostrou atividade, 4 cepas de S.aureus e as cepas de Candida foram sensíveis ao óleo essencial de R. graveolens. CONCLUSÃO: Algumas plantas apresentaram resultados satisfatórios, dependendo do agente etiológico, porém se faz necessário estudos mais detalhados, para melhorar o aproveitamento destas plantas.

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