scholarly journals Methods for Candida albicans biofilm formation on temporary soft liner/ Métodos para a formação de biofilme de Candida albicans em reembasador macio temporário

2021 ◽  
Vol 4 (5) ◽  
pp. 21447-21459
Author(s):  
Francine Sumie Morikava ◽  
Gustavo Simão Moraes ◽  
Victoria Schlumberger Cachoeira ◽  
Mayara Precoma Ramalho ◽  
Rafaela Alves da Silva ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Cell Viability ◽  
Biofilm Formation ◽  
Artificial Saliva ◽  
Storage Conditions ◽  
Degree Of Contamination ◽  
Xtt Assay ◽  
Liner Material ◽  
Soft Liner

This study evaluated methods for the contamination of a soft liner material (Softone™) with Candida albicans biofilm. Specimens were either submitted or not to pretreatment in artificial saliva in an orbital incubator, and then held suspended in different positions (horizontal or vertical) and different storage conditions (bacteriological incubator or orbital incubator) during biofilm formation. Eight conditions were tested. All specimens were immersed in C. albicans inoculum and stored in an orbital incubator at 75 rpm or in a bacteriological incubator, both at 37ºC for 90 min. Then, they were washed in PBS, and maintained in RPMI-1640 medium under the same conditions for 48 h. The degree of contamination was determined by the XTT assay. Data were submitted to ANOVA 1-factor/Tukey HSD test (α=0.05). Specimens held horizontally in an orbital incubator showed the highest cell viability, while the ones kept vertically in a bacteriological incubator had the lowest viability (p0.0001). The best condition for C. albicans biofilm formation was obtained when specimens were not submitted to pretreatment in saliva and were held horizontally in an orbital incubator.

scholarly journals Improvement of XTT assay performance for studies involving Candida albicans biofilms

2008 ◽  
Vol 19 (4) ◽  
pp. 364-369 ◽  
Author(s):  
Wander José da Silva ◽  
Jayampath Seneviratne ◽  
Nipuna Parahitiyawa ◽  
Edvaldo Antonio Ribeiro Rosa ◽  
Lakshman Perera Samaranayake ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Cell Activity ◽  
Growth Yield ◽  
Xtt Assay ◽  
Biofilm Growth ◽  
Assay Performance ◽  
Reduction Assay ◽  
Cell Layers ◽  
Biofilm Development

2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay has been used to study Candida biofilm formation. However, considering that the XTT reduction assay is dependent on cell activity, its use for evaluating mature biofilms may lead to inaccuracies since biofilm bottom cell layers tend to be relatively quiescent at later stages of biofilm formation. The aim of this study was to improve XTT reduction assay by adding glucose supplements to the standard XTT formulation. Candida albicans ATCC 90028 was used to form 24-, 48- and 72-h biofilms. The oxidative activity at 90, 180 and 270 min of incubation was evaluated. The control consisted of standard XTT formulation without glucose supplements, and was modified by the addition of 50, 100 and 200 mM of glucose. The XTT assay with 200 mM glucose showed more accurate and consistent readings correlating with biofilm development at 24, 48 and 72 h. Biofilm growth yield after 180 min incubation, when evaluated with the 200 mM glucose supplemented XTT, produced the most consistent readings on repetitive testing. It may be concluded that glucose supplementation of XTT could minimize variation and produce more accurate data for the XTT assay.

scholarly journals Influence of artificial saliva in biofilm formation of Candida albicans in vitro

2012 ◽  
Vol 26 (1) ◽  
pp. 24-28 ◽  
Author(s):  
Michelle Peneluppi Silva ◽  
José Chibebe Junior ◽  
Adeline Lacerda Jorjão ◽  
Ana Karina da Silva Machado ◽  
Luciane Dias de Oliveira ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Artificial Saliva

scholarly journals A clinical study of adherence of candida albicans to an improved NBR denture soft lining material

2015 ◽  
Vol 05 (03) ◽  
pp. 036-039
Author(s):  
Naeem Ahmed ◽  
Aruna J. Bhandari ◽  
Arti Saluja Sachdev ◽  
Farah Khan ◽  
Shitanshu Malhotra ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Clinical Study ◽  
Oral Hygiene ◽  
Controlled Environment ◽  
Liner Material ◽  
Colony Forming Units ◽  
Lining Material ◽  
Soft Liner ◽  
Material Sample

Abstract30 dentures selected for the study were disinfected using effervescent denture tablets. Then the dentures were trimmed 1.5mm from fitting surface and relined with NBR soft lining material. Sample was scraped from denture fitting surface after one month and two months duration and the changes in colony forming units per mm after each month's time were measured and marked. After one month's duration, 24 samples were reported sterile and 6 showed colonies of Candida albicans & Staphylococcus aureus. After 2 months duration, 28 out of 30 samples became positive with C. albicans & S. aureus colonies and aerobic spore bearing units. NBR soft liner material was sterile till one month of clinical usage so it can be prescribed as a safe soft lining material till one month of clinical usage in a controlled environment of oral hygiene

scholarly journals Plant-Derived Decapeptide OSIP108 Interferes with Candida albicans Biofilm Formation without Affecting Cell Viability

2014 ◽  
Vol 58 (5) ◽  
pp. 2647-2656 ◽  
Author(s):  
N. Delattin ◽  
K. De Brucker ◽  
D. J. Craik ◽  
O. Cheneval ◽  
M. Frohlich ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Cell Viability ◽  
Biofilm Formation

scholarly journals Effect of Propolis on the Adhesion Index, Morphology and Viability of Candida albicans Cells on Biofilm Formation

2021 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Dinda Oktia Maghfiroh ◽  
A.A. Santi Dewi ◽  
Anggie Diniayuningrum ◽  
Bambang Rahardjo ◽  
Nurdiana Nurdiana ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Light Microscopy ◽  
Cell Viability ◽  
Biofilm Formation ◽  
Ethanol Extract ◽  
Hyphal Growth ◽  
Colony Growth ◽  
Vulvovaginal Candidiasis ◽  
The One ◽  
Adhesion Index

About 70-75% of reproductive women have experienced vulvovaginal candidiasis at least once and 40-45% will have recurrences. Candida albicans is the most etiology of vulvovaginal candidiasis and is able to form biofilm that can lead to antifungal resistances and recurrences. One of the natural products that have an antibiofilm effect is propolis. This study expected that propolis from Lawang can be the one of antibiofilm agent candidates to antifungi resistant cases. This study aimed to know the antibiofilm effect of propolis ethanol extract to cell adhesion index, morphology, and viability of Candida albicans cell on biofilm formation. The adhesion index was counted on 100 epithelial cells under light microscopy (1000x). Morphology was seen using light microscopy (400x). Cell viability was examined by CFU assay. At 12.5% concentration of propolis ethanol extract, adhesion index decreased (p=0,000) and hyphal growth was inhibited. Colony growth decreased at 2.5% concentration and was not seen at 10% concentration of propolis ethanol extract (p=0,000). This results indicated that propolis ethanol extract can decrease adhesion index, failed the Candida albicans morphology transition from yeast to hyphal, and decreased Candida albicans cell viability on biofilm formation. Propolis ethanol extract is likely to be one of alternatives to recurrent vulvovaginal candidiasis treatment, especially caused by Candida albicans biofilm formation.

Effect of Propolis on the Adhesion Index, Morphology and Viability of Candida albicans Cells on Biofilm Formation

2021 ◽  
Author(s):  
Dinda Oktia Maghfiroh ◽  
A.A. Santi Dewi ◽  
Anggie Diniayuningrum ◽  
Bambang Rahardjo ◽  
Nurdiana Nurdiana ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Light Microscopy ◽  
Cell Viability ◽  
Biofilm Formation ◽  
Ethanol Extract ◽  
Hyphal Growth ◽  
Colony Growth ◽  
Vulvovaginal Candidiasis ◽  
The One ◽  
Adhesion Index

About 70-75% of reproductive women have experienced vulvovaginal candidiasis at least once and 40-45% will have recurrences. Candida albicans is the most etiology of vulvovaginal candidiasis and is able to form biofilm that can lead to antifungal resistances and recurrences. One of the natural products that have an antibiofilm effect is propolis. This study expected that propolis from Lawang can be the one of antibiofilm agent candidates to antifungi resistant cases. This study aimed to know the antibiofilm effect of propolis ethanol extract to cell adhesion index, morphology, and viability of Candida albicans cell on biofilm formation. The adhesion index was counted on 100 epithelial cells under light microscopy (1000x). Morphology was seen using light microscopy (400x). Cell viability was examined by CFU assay. At 12.5% concentration of propolis ethanol extract, adhesion index decreased (p=0,000) and hyphal growth was inhibited. Colony growth decreased at 2.5% concentration and was not seen at 10% concentration of propolis ethanol extract (p=0,000). This results indicated that propolis ethanol extract can decrease adhesion index, failed the Candida albicans morphology transition from yeast to hyphal, and decreased Candida albicans cell viability on biofilm formation. Propolis ethanol extract is likely to be one of alternatives to recurrent vulvovaginal candidiasis treatment, especially caused by Candida albicans biofilm formation.

scholarly journals Rifampicin induced virulence determinants increase Candida albicans biofilm formation

F1000Research ◽  
2013 ◽  
Vol 2 ◽  
pp. 106 ◽  
Author(s):  
Miriam Vogel ◽  
Martin Köberle ◽  
Holger Schäffler ◽  
Monika Treiber ◽  
Ingo B Autenrieth ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Bloodstream Infections ◽  
Surface Hydrophobicity ◽  
Tube Formation ◽  
Virulence Determinants ◽  
Xtt Assay ◽  
Rifampicin Treatment ◽  
Mdr1 Expression

Increased intravenous catheter use has been paralleled by increased bacterial and yeast bloodstream infection. Biofilm formation, which is associated with the cell surface hydrophobicity (CSH) phenotype, represents a major pathogenicity strategy ofCandida albicans, becoming especially important in the colonization of intravascular medical devices. Increasing evidence shows the induction of virulence factors inC. albicansby diverse substances. Therefore, we investigated whether rifampicin, an antibiotic shown to be capable of inducing MDR1 expression inC. albicansmay also promote the formation of a pathogenic biofilm. In response to 40 µg/mL rifampicin, an enhanced retention ofC. albicansSC5314 cells on polystyrene culture plates was observed by measuring increased metabolic activity by XTT assay, indicating induction of biofilm formation. Rifampicin treatment also induced fibronectin binding, cell hydrophobicity and germ tube formation. Furthermore, increased RNA and protein expression of CSH1p, a major mediator of CSH, was demonstrated. We conclude that exposure to rifampicin may result in upregulation of keyCandidavirulence determinants, potentially boosting pathogenicity and supporting biofilm formation. This finding gains clinical significance from the increasing popularity of rifampicin-coated catheters, which might provide an advantageous gateway forCandidabloodstream infections.

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