scholarly journals Characterization of Lectin from Colpomenia Sinuosa and Effect of Physico Chemical Parameters on Haemagglutination Activity

Lectin is a protein which has the ability to bind carbohydrates and named as haemagglutinin. Lectins with specific carbohydrate specificity have been purified from various plant tissues and other organisms and exploited extensively in many aspects of biochemistry and biomedicine. Similar to land plants, lectins from marine algae appear to be useful in some biological applications. Although several studies on lectins from marine algae have been reported till date, few lectins from algae have been characterized in detail. The present study was focused on the lectin isolated from C.sinuosa. The algal lectin has high sugar specificity with N-acetylglucosamine and higher enzyme activity with trypsin. This lectin was identified as CaCl2 dependent – ‘C’ type lectin and was sensitive to EDTA. Higher H.A titre value was observed with CaCl2 and the lower with MnCl2 and ZnCl2 . Significant lectin activity was observed between pH 7 to 8 and temperature between 20 to 40 O C

2015 ◽  
Vol 88 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Igor Jerković ◽  
Carlo Ignazio Giovanni Tuberoso ◽  
Goran Baranović ◽  
Zvonimir Marijanović ◽  
Marina Kranjac ◽  
...  

Food Control ◽  
2002 ◽  
Vol 13 (4-5) ◽  
pp. 329-336 ◽  
Author(s):  
Alexandre J. Cichoscki ◽  
Eunice Valduga ◽  
Alice T. Valduga ◽  
Marı́a E. Tornadijo ◽  
José M. Fresno

2018 ◽  
Vol 3 (02) ◽  
pp. 150-157
Author(s):  
Asad Amir ◽  
Neelesh Kapoor ◽  
Hirdesh Kumar ◽  
Mohd. Tariq ◽  
Mohd. Asif Siddiqui

Sandalwood is a commercially and culturally important plant species belonging to the family Santalaceae and the genus Santalum. In Indian sandalwood is renowned for its oil, which is highly rated for its sweet, fragrant, persistent aroma and the fixative property which is highly demanded by the perfume industry. For better production and varieties, requires to understanding the functions of proteins, their analysis and characterization of proteins sequences and their structures, their localizations in cell and their interaction with other functional partner. Due to limited number of in silico studies on sandalwood, in the present study we have performed in silico analysis by characterization of sandalwood proteins. Total 23 proteins were obtained and characterization using UniProtKB, identifying their physico-chemical parameters using ProtParam tool and prediction of their secondary structure elements using GOR of all 23 proteins.


2005 ◽  
Vol 107 (3) ◽  
pp. 158-166 ◽  
Author(s):  
José Luis Bernal ◽  
Juan José Jiménez ◽  
María Jesús del Nozal ◽  
Laura Toribio ◽  
María Teresa Martín

2020 ◽  
Vol 14 (7) ◽  
pp. 2452-2462
Author(s):  
Ali Mohamed Abdou Salam ◽  
Ngansoumana Ba ◽  
Ismaïla Ndour ◽  
Seyni Sane ◽  
Modou Thiaw ◽  
...  

Au Sénégal, peu d’études sur le plan planctonique ont été réalisées pour l’évaluation de l’efficacité des Aires Marines Protégées. Dans cette perspective, la caractérisation de la flore phytoplanctonique et sa distribution spatio-temporelle à l’intérieur et à l’extérieur de l’AMP de Bamboung a été réalisée en avril et en septembre 2016. Des prélèvements d’échantillons d’eau à l’aide d’un filet à plancton de 20 μm de mailles ainsi que des mesures de paramètres physico-chimiques ont été faits dans chaque station. L’analyse des données environnementales a montré l’existence d’un effet intersaison avec des valeurs plus élevées en avril excepté la température. Par ailleurs, un effet d’inter-sites a été observé uniquement en septembre pour la température, la salinité, la conductivité et l’oxygène dissout. En termes de diversité phytoplanctonique, en avril, 49 espèces réparties dans 5 classes ont été identifiées dans l’AMP contre 47 espèces et 5 classes identifiées à Diomboss et 42 espèces et 5 classes à Sangako. En septembre, 57 espèces réparties dans 5 classes ont été répertoriées dans l’AMP contre 54 espèces et 4 classes à Diomboss, et 51 espèces réparties dans 4 classes identifiées à Sangako. Les résultats seuls de la richesse spécifique du phytoplancton ne permettent pas d’apporter une contribution sur l’évaluation de l’efficacité de l’AMP de Bamboung. Mots clés : Phytoplancton, dynamique, composition taxonomique, AMP, Bamboung.   English Title: Characterization of the phytoplankton flora in the Protected Marine Area (MPA) of Bamboung and two surrounding sites (Senegal) In Senegal, few planktonic data exist in the Marine Protected Areas (AMP). In this perspective, the characterization of phytoplankton flora and its spatial and temporal distribution within and outside the Bamboung AMP was carried out during in April and in september. Water samples were taken using a 20 μm mesh plankton net and measurements of physico-chemical parameters were made at each station. The analysis of environmental data showed the existence of an inter-site effect during the two-month sampling period. In April, 49 species in 5 classes were identified in the AMP, compared to 47 species and 5 classes identified in Diomboss and 42 species and 5 classes in Sangako. In september, 57 species distributed in 5 classes were recorded in the AMP compared to 54 species distributed in 4 classes in Diomboss and 51 species and 4 classes identified in Sangako. The results of the specific richness of phytoplankton alone do not make it possible to make a contribution to the evaluation of the effectiveness of Bamboung's MPA.Keywords: Phytoplankton, dynamics, taxonomic composition, AMP; Bamboung.


e-Polymers ◽  
2004 ◽  
Vol 4 (1) ◽  
Author(s):  
Rocco Angelone ◽  
Claudia Castè ◽  
Valter Castelvetro ◽  
Francesco Ciardelli ◽  
Arturo Colligiani ◽  
...  

Abstract Synthesis and electrooptical analysis of polysiloxane and poly-N-vinyl derivatives containing indolyl groups are reported. The indole group and some of its methyl derivatives have been taken into account in order to evaluate their behaviour, with respect to that of the widely employed carbazole group, when used as photoconductive centres attached to a polymer chain. The obtained data show that photoconductivity and traps formation mechanism can be inferred as functions of the physico-chemical parameters (electric dipole moment and ionization potential) of the different groups and polymers involved. To this end such parameters have been carefully computed for a series of pyrrole, indole and carbazole derivatives. The 2,3-dimethylindole derivative appears to be particularly promising due to its electrooptical behaviour in the red absorption region where measurements have been accomplished and are shown to be consistent with the theoretical predictions.


1988 ◽  
Vol 53 (5) ◽  
pp. 1094-1101 ◽  
Author(s):  
Ernest Šturdík ◽  
Štefan Baláž ◽  
Edita Ďurčová ◽  
Marián Antalík ◽  
Pavol Sulo ◽  
...  

The characterization of relations between the assumed activity-determining physico-chemical parameters (partition coefficients, dissociation constants, reactivity to thiol compounds) of o-, m-, and p-substituted phenylhydrazonopropanedinitriles and their uncoupling effect on oxidative phosphorylation in mitochondria isolated from rat liver, Paracoccus denitrificans bacteria and animal leukemia P 388 cells have shown that from the viewpoint of action mechanism the concept of proton-translocating (pH-gradient disturbing) effect is more plausible than the modification of the corresponding membrane proteins. The dependence of the activity of the compounds studied on their physicochemical characteristics is represented by a saturation curve with a plateau starting at log P 5-6 when the uncoupling effect is plotted versus lipophilicity and by a bell-shaped curve having a maximum at pKa 4·5-5·5 with respect to basicity.


Sign in / Sign up

Export Citation Format

Share Document