OVARIAN MASQUERADES: A COMPARATIVE STUDY OF FROZEN SECTION REPORTING OF OVARIAN MASSES WITH FINAL HISTOPATHOLOGICAL DIAGNOSIS AT A TERTIARY CANCER CENTRE: A TWO YEAR STUDY.

Introduction: Intraoperative assessment of ovarian neoplasms on Frozen section (FS) as epithelial and non-epithelial types is crucial for appropriate surgical management. Accurate categorization is important as treatment plan includes cystectomy for benign tumors, extensive staging procedures for ovarian carcinomas and oophorectomy or limited surgical staging for borderline tumors especially in younger patients to preserve fertility. Objectives: To study the accuracy of ovarian FS interpretation with reference to nal histopathological (HPE) diagnosis on parafn embedded sections after analyzing by morphology and immunohistochemistry (IHC) wherever necessary. Materials and Methods:Aretrospective analysis of 170 cases of FS study on ovarian masses during the period of two years (2017, 2018) was done at a tertiary oncology center. Results: Of the total 170 cases on frozen, 74 cases were benign, 27 cases borderline and 69 cases malignant. On nal HPE 73 cases were benign, 20 cases borderline and 77 cases malignant neoplasms. The sensitivity and specicity were 95.89%, 95.87% in benign tumors, 80%, 92.66% in borderline and 88.3%, 98.92% in malignant tumors respectively. The positive and negative predictive values were 94.59%, 96.87% in benign lesions, 59.25%, 97.20% in borderline, 98.55%, and 91.08% in malignant neoplasms respectively. Conclusions:In our study there was a good concordance between frozen and nal histopathology in the diagnosis of benign and malignant ovarian neoplasms. Ovarian FS study is a reliable tool for intraoperative decision making regarding the extent of surgery

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Histopathological diagnosis of ovarian mass

Journal of Pathology of Nepal ◽

10.3126/jpn.v8i1.19448 ◽

2018 ◽

Vol 8 (1) ◽

pp. 1261-1264

Author(s):

Shahali Shadab ◽

Tadayon Tadayon

Keyword(s):

Malignant Tumors ◽

Histopathological Examination ◽

Age Groups ◽

Germ Cell Tumors ◽

Cystic Teratoma ◽

Mature Cystic Teratoma ◽

Benign Tumors ◽

Histopathological Diagnosis ◽

Hospital Data ◽

Ovarian Masses

Background: Ovarian cysts are common forms of gynecological problems that can be range from physiological cysts to highly aggressive neoplastic lesions. The purpose of this study was to investigate prevalence and frequency of different histopathological patterns of ovarian lesions and their correlation with various parameters in Ahvaz, Iran.Materials and Methods: This is the retrospective study of patients with the ovarian masses at Ahvaz Imam Khomeini Hospital from 2010 - 2015. The relevant clinical details about the patient were retrieved from hospital data. Clinical characteristics of patients such as patient's age, presenting signs and symptoms, histopathological diagnosis, mass type, mass subtype, size of cysts and ovary which is involved were noted. Results: Two hundred sixty seven specimens of ovarian tumor obtained for histopathological examination. Of these, 163(61.0%) were tumor like, 96(36.0%) were benign tumor and 8(3.0%) were malignant. The most common tumor like conditions was Corpus luteum cyst (43.4% cases), among benign and malignant tumors, mature cystic teratoma (17.2% of total) and Epithelial tumors (n=4) were most common. There is a statistically significant positive relation between age and various ovarian masses. (P= 0.002). Histopathological diagnosis wasn't correlated with ovarian involvement.Conclusion: Benign tumors are more common than malignant tumors in all age groups. Germ cell tumors followed by surface epithelial cell tumors are the commonest tumor. Mature cystic teratoma was the most common tumor. Unilaterality is more frequently seen in ovarian tumors and various tumors are seen in various age groups.

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Parotid Tumors: Fine-Needle Aspiration and/or Frozen Section

Otolaryngology ◽

10.1016/j.otohns.2008.09.013 ◽

2008 ◽

Vol 139 (6) ◽

pp. 811-815 ◽

Cited By ~ 63

Author(s):

Peter Zbären ◽

Dominique Guélat ◽

Heinz Loosli ◽

Edouard Stauffer

Keyword(s):

Sensitivity And Specificity ◽

Fine Needle Aspiration ◽

Malignant Tumors ◽

Frozen Section ◽

Needle Aspiration ◽

Benign Tumors ◽

Malignant Neoplasms ◽

Tumor Type ◽

Fine Needle ◽

Parotid Tumors

Objective The purpose of this study was to analyze and compare the value of fine-needle aspiration cytology (FNAC) and frozen section (FS) analysis in the assessment of parotid gland tumors. Study Design Chart review and cross-sectional analysis. Subjects and Methods FNAC and FS analysis of 110 parotid tumors, 68 malignancies and 42 benign tumors, were analyzed and compared with the final histopathologic diagnosis. Results The accuracy, sensitivity, and specificity of FNAC in detecting malignant tumors were 79 percent, 74 percent, and 88 percent, respectively. On FS analysis, the accuracy, sensitivity, and specificity in detecting malignant tumors were 94 percent, 93 percent, and 95 percent, respectively. The histologic tumor type was correctly diagnosed by FNAC and FS in 27 of 42 (64%) and 39 of 42 (93%) benign tumors, respectively, and in 24 of 68 (35%) and 49 of 68 (72%) malignant neoplasms, respectively. Conclusion The current analysis showed a superiority of FS compared with FNAC regarding the diagnosis of malignancy and tumor typing. FNAC alone is not prone to determine the surgical management of parotid malignancies.

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Role of imprint cytology in the diagnosis of ovarian neoplasms

Indian Journal of Pathology and Oncology ◽

10.18231/j.ijpo.2021.064 ◽

2021 ◽

Vol 8 (3) ◽

pp. 320-326

Author(s):

Archana Shivamurthy ◽

Padmapriya Jaiprakash

Keyword(s):

Germ Cell ◽

Ovarian Neoplasms ◽

Malignant Tumors ◽

Germ Cell Tumors ◽

Malignant Neoplasms ◽

Histopathological Diagnosis ◽

Imprint Cytology ◽

Tumor Type ◽

Secondary Tumors

Primary ovarian neoplasms constitute a heterogenous group of benign and malignant tumors of epithelial, sex cord–stromal, mesenchymal and germ cell origin. Secondary tumors constitute a minority. The management of benign and malignant ovarian neoplasms varies and it is here that imprint cytology plays a crucial role in diagnosis. It provides a rapid intraoperative diagnosis which will decide further treatment course for the patient. Imprint cytology of ovarian neoplasms is simple, inexpensive, challenging and provides a rapid diagnosis with excellent cellular details. The aim of the present study is to determine the role of imprint cytology in the diagnosis of ovarian neoplasms. A retrospective study was conducted in the department of pathology. A total of 53 cases were included in the study. In 48 cases, the imprint cytology findings correlated with the histopathological diagnosis. 5 cases did not correlate. Epithelial ovarian tumors accounted for the majority, (85%) followed by germ cell tumors (9.4%) and sex cord stromal tumors (5.6%). The sensitivity and specificity were 93.75% and 100% respectively. Thus imprint cytology is an effective cytological method in the diagnosis of ovarian neoplasms. Knowledge of specific cytological features for each tumor type helps in accurate diagnosis which in turn is valuable in immediate appropriate treatment and management of patients with benign or malignant neoplasms.

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p53, c-erbB2, and PCNA Status in Benign, Proliferative, and Malignant Ovarian Surface Epithelial Neoplasms

Archives of Pathology & Laboratory Medicine ◽

10.5858/1999-123-0310-pceaps ◽

1999 ◽

Vol 123 (4) ◽

pp. 310-316

Author(s):

Michael B. Anreder ◽

Scott M. Freeman ◽

Athir Merogi ◽

Susan Halabi ◽

Aizen J. Marrogi

Keyword(s):

Ovarian Neoplasms ◽

Malignant Tumors ◽

Malignant Potential ◽

Benign Tumors ◽

Malignant Neoplasms ◽

Proliferative Index ◽

Full Spectrum ◽

Ovarian Surface ◽

Low Malignant Potential ◽

Ovarian Surface Epithelial

Abstract Low malignant potential tumors of the ovary are believed to behave in a manner intermediate to their benign and malignant counterparts. However, recent evidence suggests these lesions are in fact benign and better classified as proliferative. Based on our previous work and evaluating p53, c-erbB2, and PCNA status in a full spectrum of ovarian surface epithelial tumors, with emphasis on low malignant potential tumors, we tested this hypothesis. Immunohistochemical stains with monoclonal antibodies were used on 75 archival ovarian neoplasms. The results demonstrated anti-p53 reactivity in 30 carcinomas (40%), 2 of which were proliferative, and no reactivity in the benign tumors. Overexpression of c-erbB2 was seen in 31 malignant neoplasms (64.5%), 4 of which were proliferative (22.1%), and none in benign tumors. The PCNA proliferative index showed means of 42.8%, 22.8%, and 14.9% with benign, low malignant potential, and malignant tumors, respectively. Predicting immunoreactivity in carcinomas for anti-PCNA (Student t test), anti-p53, and anti–c-erbB2 (Pearson χ2 test) versus a lack of immunoreactivity in proliferative tumors indicate P values of .001, <.001, and <.001, respectively. These data show significant differences in the expression of these markers in ovarian tumors and suggest a possible role for these oncogenes as supplemental tools in diagnostic pathology. Further, our findings also support the designation of proliferative as opposed to the current nomenclature of low malignant potential tumors.

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A cytohistological study of p53 overexpression in ovarian neoplasms

South Asian Journal of Cancer ◽

10.4103/2278-330x.103711 ◽

2012 ◽

Vol 01 (02) ◽

pp. 59-62 ◽

Cited By ~ 2

Author(s):

Monisha Choudhury ◽

Seema Goyal ◽

Mukta Pujani ◽

Meenu Pujani

Keyword(s):

Ovarian Neoplasms ◽

Malignant Tumors ◽

Biological Significance ◽

Diagnostic Value ◽

Ovarian Tumors ◽

Benign Tumors ◽

Histopathological Diagnosis ◽

Imprint Cytology ◽

P53 Overexpression ◽

P53 Expression

Abstract Background: The present study was undertaken to evaluate the diagnostic accuracy of imprint cytology in ovarian neoplasms, investigate the biological significance of p53 expression in malignant ovarian tumors and correlate it with histological type, grade and stage of tumor. Material and Methods: A total of 50 cases including 25 prospective and 25 retrospective cases were studied. Imprint cytology was performed on 25 ovarian tumors and compared with histopathological diagnosis. p53 immunohistochemistry was performed on all 50 cases. Results: On immunohistochemistry, all the benign tumors were negative for p53 while 42% of primary ovarian malignant tumors were positive. p53 expression was found to have a diagnostic value in differentiating benign from malignant tumors. p53 overexpression did not show any significant correlation with prognostic factors as stage of disease, grade of differentiation and type of tumor. Conclusion: The present study confirms the importance of p53 tumor suppressor gene expression as documented by immunohistochemistry in the differentiation of malignant and benign ovarian tumors.

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Evaluation of a step-by-step approach to frozen section diagnosis in ovarian masses

Asian Journal of Medical Sciences ◽

10.3126/ajms.v12i12.39635 ◽

2021 ◽

Vol 12 (12) ◽

pp. 133-139

Author(s):

Ashumi Gupta ◽

Neelam Jain

Keyword(s):

Sensitivity And Specificity ◽

Malignant Tumors ◽

Frozen Section ◽

Tertiary Care ◽

False Negative ◽

Significant Proportion ◽

False Negative Rate ◽

Accurate Diagnosis ◽

Care Hospital ◽

Ovarian Masses

Background: Ovarian cancer forms a significant proportion of cancer-related mortality in females. It is often detected late due to non-specific clinical presentation. Radiology and tumor markers may indicate an ovarian mass. However, exact diagnosis requires pathological evaluation, which may not be possible before surgery. Intraoperative frozen section (FS) is, therefore, an important modality for the diagnosis of ovarian masses. Aims and Objectives: This study was conducted to study step-by-step approach along with diagnostic utility and accuracy of intraoperative FS in diagnosis of ovarian masses. Materials and Methods: Retrospective comparative analysis was done to determine the diagnostic accuracy of FS as compared to routine histopathology in the pathology department of a tertiary care hospital. Diagnostic categorization was done into benign, borderline, and malignant. Overall accuracy, sensitivity, and specificity of FS technique were calculated. Results: Out of 51 cases, FS analysis yielded accurate diagnosis in 94.1% of ovarian masses. Intraoperative FS had a sensitivity of 94.7%, specificity of 96.9%, 3.1% false-positive rate, and 5.3% false-negative rate in malignant tumors. In benign lesions, FS had 91.7% sensitivity and 100% specificity. FS had 75% sensitivity and 96.4% specificity in cases of borderline tumors. Conclusion: FS is a fairly accurate technique for intraoperative evaluation of ovarian masses. It can help in deciding the extent of surgery. It distinguishes benign and malignant tumors in most cases with high sensitivity and specificity. A methodical approach is useful in determining accurate diagnosis on FS diagnosis.

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HISTOPATHOLOGICAL SPECTRUM OF SKIN LESIONS: A TWO YEAR RETROSPECTIVE STUDY.

10.36106/ijsr/6201091 ◽

2021 ◽

pp. 26-29

Author(s):

Shruti Shemawat ◽

Sakshi Apurva ◽

D.P Soni ◽

Saurabh Soni

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Relative Frequency ◽

Malignant Tumors ◽

Skin Lesions ◽

Benign Tumors ◽

Histopathological Diagnosis ◽

Neoplastic Lesions ◽

Skin Biopsies ◽

Age And Sex

INTRODUCTION: The skin being largest organ of the body has vast spectrum of disorders which can be difcult to diagnose correctly solely on the basis of clinical features. Hence histopathological examination is necessary to categorise skin lesions. The aim was to study relative frequency of various skin lesions and distribution of these lesions according to age and sex. METHODS: This is a retrospective descriptive hospital based study. The skin biopsies samples which came in the duration of two years from January 2019 to December 2020 at the Department of Pathology, Sardar Patel Medical college and associated group of hospitals, Bikaner, Rajasthan were taken in this study. All skin biopsies that showed denite histopathological diagnosis were included. After proper xing and staining procedures these lesions were examined under light microscopy and categorized as non-neoplastic and neoplastic. Relative frequency of various lesions, distribution of lesions according to age and sex was analyzed. The data collected was tabulated, interpreted and compared with other similar studies. RESULTS: Out of 346 patients, incidence of neoplastic lesions 259 (74.9%) were higher than non-neoplastic lesions 87(25.1%). Males were affected more compared to females with male to female ratio 1.45:1. Non-neoplastic lesions were mostly caused because of infectious etiologies among which leprosy was the most common infection. Keratinocytic tumors 99(52.2%) constituted most common type of neoplastic lesion. Benign tumors 191(73.7%) outnumbered malignant tumors 68(26.3%). The cases of benign tumors were seen more in younger population while that of malignant tumors were seen in older age groups. Among the keratinocytic type of malignant skin tumors squamous cell carcinoma (63.5%) was the most common variant which was followed by basal cell carcinoma 19(36.5%). Male predominance was observed in both squamous cell carcinoma and basal cell carcinoma. CONCLUSION: A wide heterogenesity of skin lesions was observed in the present study . These skin lesions were mostly affecting age group of 10-30 years. Inspite of extensive programmes and research, leprosy and tuberculosis remains a rampant cause of infectious non-neoplastic skin lesions. Sometimes ignorance by patient for a very small appearing skin lesions becomes life threatening. Hence early clinician consultation with proper examination and accurate histopathological diagnosis becomes the mainstay in early treatment and recovery.

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Accuracy of Intraoperative Examination in Central Nervous System Lesions: A Study of 133 Cases

Acta Cytologica ◽

10.1159/000495175 ◽

2019 ◽

Vol 63 (3) ◽

pp. 224-232

Author(s):

Ludmila Barbosa de Souza Balsimelli ◽

Jamille Costa de Oliveira ◽

Flora Ávila Adorno ◽

Clarissa Almeida Brites ◽

Giuliano Stefanello Bublitz ◽

...

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Frozen Section ◽

Final Diagnosis ◽

World Health ◽

Histopathological Diagnosis ◽

Low Grade ◽

Predictive Values ◽

Health Organization ◽

Cns Lesions

Objective: Intraoperative examination is a highly valuable tool for the evaluation of central nervous system (CNS) lesions, helping the neurosurgeon to determine the best surgical management. This study aimed to evaluate the accuracy and to analyze the diagnostic disagreements and pitfalls of the intraoperative examinations through correlation with the final histopathological diagnosis in CNS lesions. Study Design: Retrospective analysis of intraoperative examination of CNS lesions and their final diagnosis obtained during 16 consecutive years. All diagnoses were reviewed and classified according to World Health Organization (WHO) grading for CNS tumors. Squash was performed in 119 cases, while frozen section and both methods were done in 7 cases each. Results: Among the 133 intraoperative examinations considered, 114 (85.7%) presented concordance and 19 (14.3%) diagnostic disagreement when compared with subsequent histopathological examinations. The sensitivity and specificity for the detection of neoplasia in intraoperative examination was 98 and 94%, respectively. The positive and negative predictive values were 99 and 88%, respectively. The accuracy for neoplastic and nonneoplastic disease was 85.7%. Disagreements were more frequent among low-grade (WHO grades I and II) neoplasms and nonmalignant cases. Conclusions: Our results showed good accuracy of the intraoperative assessments for diagnosis of CNS lesions, particularly in high-grade (grades III and IV) lesions and metastatic neoplasms.

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Diagnostic accuracy of computed tomography to identify adenomas among adrenal incidentalomas in an endocrinological population

Acta Endocrinologica ◽

10.1530/eje-17-1056 ◽

2018 ◽

Vol 178 (5) ◽

pp. 439-446 ◽

Cited By ~ 8

Author(s):

M Marty ◽

D Gaye ◽

P Perez ◽

C Auder ◽

M L Nunes ◽

...

Keyword(s):

Computed Tomography ◽

Malignant Tumors ◽

Correlation Coefficients ◽

University Hospital ◽

Benign Tumors ◽

Adrenal Incidentalomas ◽

Reference Standard ◽

Quantitative Ct ◽

Endocrine Society ◽

Predictive Values

Context The recent recommendations of the European Endocrine Society states that the performance of computed tomography (CT) to characterize ‘true' adrenal incidentalomas (AIs) remains debatable. Objective To determine relevant thresholds for usual CT parameters for the diagnosis of benign tumors using robust reference standard among a large series of ‘true’ AIs recruited in an endocrinological setting. Design Retrospective study of 253 AIs in 233 consecutive patients explored in a single university hospital: 183 adenomas, 33 pheochromocytomas, 23 adrenocortical carcinomas, 5 other malignant tumors and 9 other benign tumors. Reference standard was histopathology in 118 AIs, biological diagnosis of pheochromocytoma in 2 AIs and size stability after at least 1 year of follow-up in 133 AIs. Methods Sensitivity, specificity and positive and negative predictive values were estimated for various thresholds of size, unenhanced attenuation (UA), relative and absolute wash-out (RPW, APW) of contrast media. 197 scans were reviewed independently in a blinded fashion by two expert radiologists to assess inter-observer reproducibility of measurements. Results Criteria associated with a 100% positive predictive value for the diagnosis of benign AI were: a combination of size and UA: 30 mm and 20 HU or 40 mm and 15 HU, respectively; RPW >53%; and APW >78%. Non-adenomatous AIs with rapid contrast wash-out were exclusively benign pseudocysts and pheochromocytomas, suggesting that classical thresholds of 60% and 40% for APW and RPW, respectively, can be safely used for patients with normal metanephrine values. Inter-observer reproducibility of all parameters was excellent (intra-class correlation coefficients: 0.96–0.99). Conclusions Our study, the largest conducted in AIs recruited in an endocrinological setting, suggests safe thresholds for quantitative CT parameters to avoid false diagnoses of benignity.

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Spontaneous Tumors and Non-Neoplastic Proliferative Lesions in Pet Degus (Octodon degus)

Veterinary Sciences ◽

10.3390/vetsci7010032 ◽

2020 ◽

Vol 7 (1) ◽

pp. 32

Author(s):

Tanja Švara ◽

Mitja Gombač ◽

Alessandro Poli ◽

Jožko Račnik ◽

Marko Zadravec

Keyword(s):

Malignant Tumors ◽

Single Case ◽

Case Reports ◽

Hepatocellular Adenoma ◽

Benign Tumors ◽

Malignant Neoplasms ◽

Octodon Degus ◽

Hind Limbs ◽

Neoplastic Lesion ◽

Veterinary Faculty

In recent years, degus (Octodon degus), rodents native to South America, have been becoming increasingly popular as pet animals. Data about neoplastic diseases in this species are still sparse and mainly limited to single-case reports. The aim of this study was to present neoplastic and non-neoplastic proliferative changes in 16/100 pet degus examined at the Veterinary Faculty University of Ljubljana from 2010 to 2015 and to describe the clinic-pathological features of these lesions. Twenty different lesions of the integumentary, musculoskeletal, genitourinary and gastrointestinal systems were diagnosed: amongst these were 13 malignant tumors, six benign tumors, and one non-neoplastic lesion. Cutaneous fibrosarcoma was the most common tumor (7/16 degus). It was detected more often in females (6/7 degus) and lesions were located mainly in hind limbs. The gastrointestinal tract was frequently affected, namely with two malignant neoplasms - an intestinal lymphoma and a mesenteric mesothelioma, four benign tumors – two biliary cystadenomas, an oral squamous papilloma and a hepatocellular adenoma, and a single non-neoplastic proliferative lesion. In one animal, two organic systems were involved in neoplastic lesions.

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