scholarly journals Molecular genetic analysis of West Nile virus variants circulating in European Russia between 2010 and 2019

2021 ◽  
Vol 98 (3) ◽  
pp. 308-318
Author(s):  
A. A. Baturin ◽  
G. A. Tkachenko ◽  
M. L. Ledeneva ◽  
L. V. Lemasova ◽  
O. S. Bondareva ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Molecular Genetic Analysis ◽  
Diagnostic Methods ◽  
European Russia ◽  
The South ◽  
West Nile ◽  
Genetic Lineages ◽  
Lineage 2 ◽  
Viral Lineage ◽  
South Of European Russia

Aim. A study of the prevalence of West Nile virus (WNV) genetic lineages and genovariants in the south of European Russia between 2010 and 2019.Materials and methods. The study was carried out on 311 WNV containing biological samples from patients, vectors and reservoirs of infection. WNV typing was carried out using reverse transcription and real-time polymerase chain reaction with designed three pairs of primers and three probes and by the sequencing of the 277 bp WNV genome region corresponding to the 5'-untranslated region and locus of the polyprotein gene encoding the capsid protein C. Sequencing results were analyzed using the Nucleotide BLAST software (NCBI).Results. As a result of typing, out of 311 WNV RNA isolates taken for the study, 15 (4.82%) were assigned to lineage 1 (from Astrakhan and Volgograd regions, Krasnodar and Stavropol Territories, Republic of Tatarstan), 285 (91.64%) to lineage 2 (from Astrakhan, Volgograd, Voronezh, Kursk, Lipetsk, Penza, Rostov and Saratov regions, Krasnodar and Stavropol Territories, Republics of Kalmykia and Crimea), and 11 (3.54%) to lineage 4 (from the Volgograd region, Republics of Kalmykia and Crimea). The predominance of viral lineage 2 was demonstrated. The identified isolates of the viral lineage 1 belonged to the «Astrakhan» variant, isolates of lineage 2 belonged to «Russian» and «European» variants. Previously uncommon WNV variants of lineages 1 and 4 were also found.Conclusion. Lineage 2 of WNV prevailed in the south of European Russia in the last decade. The «Russian» variant is most common and its area is expanding. The circulation of various WNV genetic lineages in Russia indicates the need for further study of their spread and improving diagnostic methods and test systems for identifying and differentiating pathogen strains.

scholarly journals First International Proficiency Study on West Nile Virus Molecular Detection

2006 ◽  
Vol 52 (10) ◽  
pp. 1851-1854 ◽  
Author(s):  
Matthias Niedrig ◽  
Sonja Linke ◽  
Herve Zeller ◽  
Christian Drosten
Keyword(s):  
West Nile Virus ◽  
Molecular Detection ◽  
Probit Analysis ◽  
Strain Identification ◽  
External Quality Control ◽  
West Nile ◽  
External Quality ◽  
Genetic Lineages ◽  
Lineage 2 ◽  
Positive Results

Abstract Background: West Nile virus (WNV) molecular detection is being conducted by a growing number of laboratories, but the degree of proficiency may vary between them. External quality control is needed. Methods: We have conducted an international quality assurance study on WNV molecular detection. Participating laboratories tested noninfectious samples inactivated by heat and gamma irradiation. Participants received 7 coded lyophilized samples containing WNV of genetic lineages 1a, 1b, and 2 at 2600 to 18 000 000 RNA copies/mL, 3 samples containing heterologous flaviviruses, and 2 negative samples. Results: Thirty laboratories participated. The average laboratory achieved 50% detection probability from 7762 copies/mL onward (probit analysis; 95% CI = 1174–24547 copies/mL). Lineages 1a and 1b were detected with equal efficiencies, but the lineage 2 strain (Ug37) was detected at significantly lower rates. Only 27% of participants were able to detect the 6 samples containing ≥1.8 × 104 copies/mL. Three laboratories generated false-positive results in negative samples. Six of 30 laboratories reported correct strain identification in 3 samples containing non-WNV flaviviruses. We observed a significant positive correlation between the capability of detecting non-WNV flaviviruses and detecting WNV lineage 2. Conclusions: Most participants showed good performance in detecting lineage 1 WNV, the predominant virus in the Northern Hemisphere. The inability of some laboratories to detect even highly concentrated lineage 2 WNV downgraded the overall outcome. The lineage 2 material received through this study will provide laboratories with the necessary template for improving their assays. Such material is otherwise hard to obtain.

Zoya Il'inichna Glezer – life and scientific achievements

2019 ◽  
pp. 318-323
Author(s):  
Zinaida V. Pushina ◽  
Galina V. Stepanova ◽  
Ekaterina L. Grundan
Keyword(s):  
Western Siberia ◽  
Kara Sea ◽  
European Russia ◽  
Great Contribution ◽  
The South ◽  
The Creation ◽  
The 1960S ◽  
Scientific Achievements ◽  
South Of European Russia

Zoya Ilyinichna Glezer is the largest Russian micropaleontologist, a specialist in siliceous microfossils — Cenozoic diatoms and silicoflagellates. Since the 1960s, she systematically studied Paleogene siliceous microfossils from various regions of the country and therefore was an indispensable participant in the development of unified stratigraphic schemes for Paleogene siliceous plankton of various regions of the USSR. She made a great contribution to the creation of the newest Paleogene schemes in the south of European Russia and Western Siberia, to the correlations of the Paleogene deposits of the Kara Sea.

scholarly journals Contrasted Epidemiological Patterns of West Nile Virus Lineages 1 and 2 Infections in France from 2015 to 2019

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 908 ◽  
Author(s):  
Cécile Beck ◽  
Isabelle Leparc Goffart ◽  
Florian Franke ◽  
Gaelle Gonzalez ◽  
Marine Dumarest ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
West Nile Virus ◽  
Invasive Disease ◽  
Northern Italy ◽  
Breaking Point ◽  
Organ Donors ◽  
West Nile ◽  
Organ Donations ◽  
History Of ◽  
Lineage 2

Since 2015, annual West Nile virus (WNV) outbreaks of varying intensities have been reported in France. Recent intensification of enzootic WNV circulation was observed in the South of France with most horse cases detected in 2015 (n = 49), 2018 (n = 13), and 2019 (n = 13). A WNV lineage 1 strain was isolated from a horse suffering from West Nile neuro-invasive disease (WNND) during the 2015 episode in the Camargue area. A breaking point in WNV epidemiology was achieved in 2018, when WNV lineage 2 emerged in Southeastern areas. This virus most probably originated from WNV spread from Northern Italy and caused WNND in humans and the death of diurnal raptors. WNV lineage 2 emergence was associated with the most important human WNV epidemics identified so far in France (n = 26, including seven WNND cases and two infections in blood and organ donors). Two other major findings were the detection of WNV in areas with no or limited history of WNV circulation (Alpes-Maritimes in 2018, Corsica in 2018–2019, and Var in 2019) and distinct spatial distribution of human and horse WNV cases. These new data reinforce the necessity to enhance French WNV surveillance to better anticipate future WNV epidemics and epizootics and to improve the safety of blood and organ donations.

Emergence of West Nile Virus Lineage-2 in Resident Corvids in Istanbul, Turkey

2021 ◽  
Author(s):  
Ozge Erdogan Bamac ◽  
Utku Y. Cizmecigil ◽  
Asli Mete ◽  
Aysun Yilmaz ◽  
Ozge Aydin ◽  
...  
Keyword(s):  
West Nile Virus ◽  
West Nile ◽  
Lineage 2

scholarly journals West Nile virus lineage 2 in Romania, 2015–2016: co-circulation and strain replacement

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Ani Ioana Cotar ◽  
Elena Fălcuță ◽  
Sorin Dinu ◽  
Adriana Necula ◽  
Victoria Bîrluțiu ◽  
...  
Keyword(s):  
West Nile Virus ◽  
West Nile ◽  
Lineage 2

scholarly journals Lineage 2 West Nile Virus as Cause of Fatal Neurologic Disease in Horses, South Africa

2009 ◽  
Vol 15 (6) ◽  
pp. 877-884 ◽  
Author(s):  
Marietjie Venter ◽  
Stacey Human ◽  
Dewald Zaayman ◽  
Gertruida H. Gerdes ◽  
June Williams ◽  
...  
Keyword(s):  
South Africa ◽  
West Nile Virus ◽  
Neurologic Disease ◽  
West Nile ◽  
Lineage 2

scholarly journals New records of megachilid bees (Hymenoptera: Megachilidae) from the North Caucasus and the south of European Russia

2020 ◽  
Vol 16 (2) ◽  
pp. 225-331
Author(s):  
A.V. Fateryga ◽  
◽  
M.Yu. Proshchalykin ◽  
Keyword(s):  
New Records ◽  
Distinct Species ◽  
European Part ◽  
European Russia ◽  
North Caucasus ◽  
The South ◽  
The North ◽  
The Family ◽  
European Part Of Russia ◽  
South Of European Russia

New data on 22 species of bees of the family Megachilidae from the North Caucasus and the south of European Russia are reported. Six species are new to Russia: Hoplitis curvipes (Morawitz, 1871), Osmia cinerea Warncke, 1988, O. ligurica Morawitz, 1868, O. cyanoxantha Pérez, 1879, Protosmia glutinosa (Giraud, 1871), and Coelioxys mielbergi Morawitz, 1880. Hoplitis turcestanica (Dalla Torre, 1896), sp. resurr. is treated as a distinct species, not a junior synonym of H. caularis (Morawitz, 1875). Megachile albocristata Smith, 1853 and M. alborufa Friese, 1911 are listed instead of previously recorded M. lefebvrei (Lepeletier de Saint-Fargeau, 1841) and M. pyrenaica (Lepeletier de Saint-Fargeau, 1841), respectively. Fourteen new regional records are reported: seven species are new to the North Caucasus, five ones are new to the south of European Russia, and two species are new to the European part of Russia as a whole. The numbers of megachilid bee species currently known in Russia, the North Caucasus, and the south of European Russia are 217, 130, and 71, respectively. The lectotype of Osmia proxima Morawitz, 1875 is designated.

Sign in / Sign up

Export Citation Format

Share Document