DEVELOPMENT OF APPROACHES FOR DETECTION OF GENOME-INTEGRATED PROVIRAL DNA OF THE HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) IN ULTRA LOW CONCENTRATIONS USING THE CRISPR/CAS SYSTEM
Keyword(s):
For the effective functioning of supervisory and health monitoring services, it is necessary to introduce modern molecular technologies into their practice. Therefore, the task of developing new effective methods for detecting pathogen, for example HIV, based on CRISPR/CAS genome editing systems, remains urgent. In the present work, guide RNAs and specific oligonucleotides were developed for preliminary amplification of highly conserved regions of the HIV-1 genome. The developed guide RNAs make it possible to detect single copies of HIV-1 proviral DNA in vitro as part of CRISPR/CAS ribonucleoprotein complexes in biological samples after preliminary amplification.
2001 ◽
Vol 75
(15)
◽
pp. 6941-6952
◽
1997 ◽
Vol 41
(5)
◽
pp. 1082-1093
◽
2002 ◽
Vol 76
(3)
◽
pp. 1015-1024
◽
2003 ◽
Vol 77
(1)
◽
pp. 291-300
◽
2013 ◽
Vol 94
(2)
◽
pp. 354-359
◽
2007 ◽
Vol 51
(11)
◽
pp. 4036-4043
◽
1999 ◽
Vol 43
(8)
◽
pp. 1835-1844
◽
Keyword(s):
2007 ◽
Vol 51
(9)
◽
pp. 3075-3080
◽