Characterization of Coagulase Negative Staphylococci Isolated from Dairy Goats in Germany

N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signals in Gram-negative bacteria. Many genes encoding AHL-degrading enzymes have been cloned and characterized in various microorganisms. Coagulase-negative staphylococci (CNS) are present on the skin of animals and are considered low-virulent species. The AHL-lactonase gene homologue, ahlS, was present in the genomes of the CNS strains Staphylococcus carnosus, Staphylococcus haemolyticus, Staphylococcus saprophyticus, and Staphylococcus sciuri. We cloned the candidate ahlS homologue from six CNS strains into the pBBR1MCS5 vector. AhlS from the CNS strains showed a higher degrading activity against AHLs with short acyl chains compared to those with long acyl chains. AhlS from S. sciuri was expressed and purified as a maltose-binding protein (MBP) fusion. Pseudomonas aeruginosa is an opportunistic pathogen that regulates several virulence factors such as elastase and pyocyanin by quorum-sensing systems. When MBP-AhlS was added to the culture of P. aeruginosa PAO1, pyocyanin production and elastase activity were substantially reduced compared to those in untreated PAO1. These results demonstrate that the AHL-degrading activity of AhlS from the CNS strains can inhibit quorum sensing in P. aeruginosa PAO1.

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An Overview of Specific Pathogens in Goat Mastitis

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Veterinary Medicine ◽

10.15835/buasvmcn-vm:2019.0025 ◽

2019 ◽

Vol 76 (2) ◽

pp. 118

Author(s):

Cristiana Ștefania NOVAC ◽

Sanda ANDREI ◽

Nicodim Iosif FIȚ

Keyword(s):

Somatic Cell Count ◽

Goat Milk ◽

Subclinical Mastitis ◽

Clinical Mastitis ◽

Economic Consequences ◽

The Other ◽

Aetiological Agent ◽

Dairy Goats ◽

Coagulase Negative Staphylococci ◽

Intramammary Infections

Goat milk ranks fourth in terms of global milk production and lately it has become increasingly popular among consumers. Unfortunately, mastitis is one of the most common diseases that affects dairy goats, with serious economic consequences and food safety matters. The prevalence of clinical mastitis is lower than 5% and the main aetiological agent is S. aureus. On the other hand, the prevalence of subclinical mastitis is between 5-30%, with coagulase negative staphylococci (CNS) representing the most often isolated microorganisms.The aim of this paper is to highlight the main aspects regarding the aetiology of goat mastitis, as well as the importance of the milk somatic cell count (MSCC) in the diagnosis process. Although the inflammation of the mammary gland in goats is not as frequently diagnosed compared to cow mastitis, there are several aspects worth discussing in order to fully understand the pathogenesis of intramammary infections.

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Characterization of methicillin-resistant coagulase-negative staphylococci in milk from cows with mastitis in Brazil

Antonie van Leeuwenhoek ◽

10.1007/s10482-014-0185-5 ◽

2014 ◽

Vol 106 (2) ◽

pp. 227-233 ◽

Cited By ~ 20

Author(s):

Nathalia C. C. Silva ◽

Felipe F. Guimarães ◽

Marcela de P. Manzi ◽

Elena Gómez-Sanz ◽

Paula Gómez ◽

...

Keyword(s):

Coagulase Negative Staphylococci ◽

Methicillin Resistant

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Characterization of biofilm producing methicillin resistant coagulase negative Staphylococci from India

Acta Microbiologica et Immunologica Hungarica ◽

10.1556/030.2021.01538 ◽

2021 ◽

Keyword(s):

Biofilm Formation ◽

Resistance Rate ◽

Coagulase Negative Staphylococci ◽

Methicillin Resistant ◽

Antimicrobial Susceptibility Pattern ◽

Healthcare Settings ◽

Staphylococcus Capitis ◽

Staphylococcus Epidermis ◽

Staphylococcus Hominis

Abstract Methicillin-resistant coagulase-negative staphylococci (MR-CoNS) cause infectious diseases due to their potential to form biofilm and further colonization in hospital materials. This study evaluated the antibiotic susceptible phenotypes, biofilm-producing ability, and biofilm-associated genes (mecA, icaAD, bap, cna, and fnbA). Biofilm formation was detected through Congo red agar (CRA) method and MTP method. The presence of biofilm and associated genes in MR-CoNS were detected by PCR. A total of 310 (55.95%) isolates produced the biofilm. Among these isolates, Staphylococcus haemolyticus (34.83%), Staphylococcus epidermis (31.93%), Staphylococcus capitis (16.77%), Staphylococcus cohnii (10.96%), and Staphylococcus hominis (5.48%) were identified. The antimicrobial susceptibility pattern of CoNS isolates indicated resistance to cefoxitin (100%), erythromycin (94.8%), ciprofloxacin (66.7%), sulfamethoxazole/trimethoprim (66.7%), gentamicin (66.12%), and clindamycin (62.9%). Resistance rate to mupirocin was 48.5% in S. epidermidis and 38.9% in S. haemolyticus isolates. All isolates were sensitive to vancomycin and linezolid. The prevalence rates of icaAD, bap, fnbA, and cna were 18.06%, 12.5%, 47.4%, and 27.4%, respectively. icaAD and bap genes were detected in 18.06% and 12.5% of MR-CoNS isolates. fnbA and cna genes were detected in 47.41% and 27.41% of MRCoNS isolates. icaAD positive strains exhibited a significant increase in the biofilm formation compared with those that lacked icaAD (0.86 (0.42, 1.39) versus 0.36 (0.14, 0.75), respectively; P < 0.001). In conclusion, the majority of MR-CoNS isolates were biofilm producers, and S. capitis, which possessed icaAD genes, ranked as the great biofilm producer than other Staphylococcus. The study’s findings are important to form a strategy to control biofilm formation as an alternative strategy to counter the spread of MR-CoNS in healthcare settings.

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Antimicrobial resistance and genetic characterization of coagulase-negative staphylococci from bovine mastitis milk samples in Korea

Journal of Dairy Science ◽

10.3168/jds.2019-17028 ◽

2019 ◽

Vol 102 (12) ◽

pp. 11439-11448 ◽

Cited By ~ 2

Author(s):

Su-Jeong Kim ◽

Dong Chan Moon ◽

Seung-Chun Park ◽

Hee Young Kang ◽

Seok Hyeon Na ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Genetic Characterization ◽

Bovine Mastitis ◽

Coagulase Negative Staphylococci ◽

Milk Samples

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Genotypic Characterization of Coagulase-negative Staphylococci Isolated from Sheep Milk in Slovakia

Acta Veterinaria Brno ◽

10.2754/avb201079020269 ◽

2010 ◽

Vol 79 (2) ◽

pp. 269-275 ◽

Cited By ~ 6

Author(s):

Ivana Pilipčincová ◽

Mangesh Bhide ◽

Eva Dudriková ◽

Milan Trávniček

Keyword(s):

Molecular Characterization ◽

Gel Electrophoresis ◽

Biochemical Characterization ◽

Pulse Field ◽

Coagulase Negative Staphylococci ◽

Pulse Field Gel Electrophoresis ◽

Milk Samples ◽

Sheep Milk ◽

Epidemiological Situation

Hitherto very few reports are available presenting identification and molecular characterization of the coagulase negative staphylococci (CNS) from sheep milk in the subclinical stage of mastitis. Furthermore, very scanty data are available on the epidemiological status of CNS in different Slovak provinces. Milk samples from 54 sheep farms located in eastern Slovak region were screened. A total 240 CNS were identified with series of biochemical testes (STAPH-API) and subjected further for genotyping with the help of pulse field gel electrophoresis (PFGE). The most frequently occurring CNS species according the biochemical characterization were:S. epidermidis(36.3 %),S. caprae(21.3 %),S. hominis(6.6 %),S. chromogenes(6.3 %),S. xylosus(5.8 %),S. warneri(5.0 %) andS. capitis(4.6 %). Further PFGE-based characterization of these isolates revealed six pulsotypes of theS. epidermidis, two ofS. caprae, three ofS. chromogenes, nine ofS. hominis, five ofS. capitisand seven ofS. xylosus. These results contribute to knowledge of the epidemiological situation of the CNS from the subclinical form of mastitis in Slovakia.

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