Characterization of methicillin-resistant coagulase-negative staphylococci in milk from cows with mastitis in Brazil

2014 ◽  
Vol 106 (2) ◽  
pp. 227-233 ◽  
Author(s):  
Nathalia C. C. Silva ◽  
Felipe F. Guimarães ◽  
Marcela de P. Manzi ◽  
Elena Gómez-Sanz ◽  
Paula Gómez ◽  
...  
Keyword(s):  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant

Characterization of biofilm producing methicillin resistant coagulase negative Staphylococci from India

2021 ◽  
Keyword(s):  
Biofilm Formation ◽  
Resistance Rate ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Antimicrobial Susceptibility Pattern ◽  
Healthcare Settings ◽  
Staphylococcus Capitis ◽  
Staphylococcus Epidermis ◽  
Staphylococcus Hominis

Abstract Methicillin-resistant coagulase-negative staphylococci (MR-CoNS) cause infectious diseases due to their potential to form biofilm and further colonization in hospital materials. This study evaluated the antibiotic susceptible phenotypes, biofilm-producing ability, and biofilm-associated genes (mecA, icaAD, bap, cna, and fnbA). Biofilm formation was detected through Congo red agar (CRA) method and MTP method. The presence of biofilm and associated genes in MR-CoNS were detected by PCR. A total of 310 (55.95%) isolates produced the biofilm. Among these isolates, Staphylococcus haemolyticus (34.83%), Staphylococcus epidermis (31.93%), Staphylococcus capitis (16.77%), Staphylococcus cohnii (10.96%), and Staphylococcus hominis (5.48%) were identified. The antimicrobial susceptibility pattern of CoNS isolates indicated resistance to cefoxitin (100%), erythromycin (94.8%), ciprofloxacin (66.7%), sulfamethoxazole/trimethoprim (66.7%), gentamicin (66.12%), and clindamycin (62.9%). Resistance rate to mupirocin was 48.5% in S. epidermidis and 38.9% in S. haemolyticus isolates. All isolates were sensitive to vancomycin and linezolid. The prevalence rates of icaAD, bap, fnbA, and cna were 18.06%, 12.5%, 47.4%, and 27.4%, respectively. icaAD and bap genes were detected in 18.06% and 12.5% of MR-CoNS isolates. fnbA and cna genes were detected in 47.41% and 27.41% of MRCoNS isolates. icaAD positive strains exhibited a significant increase in the biofilm formation compared with those that lacked icaAD (0.86 (0.42, 1.39) versus 0.36 (0.14, 0.75), respectively; P < 0.001). In conclusion, the majority of MR-CoNS isolates were biofilm producers, and S. capitis, which possessed icaAD genes, ranked as the great biofilm producer than other Staphylococcus. The study’s findings are important to form a strategy to control biofilm formation as an alternative strategy to counter the spread of MR-CoNS in healthcare settings.

The epidemiology and molecular characterization of methicillin-resistant staphylococci sampled from a healthy Jordanian population

2013 ◽  
Vol 141 (11) ◽  
pp. 2384-2391 ◽  
Author(s):  
A. G. AL-BAKRI ◽  
H. AL-HADITHI ◽  
V. KASABRI ◽  
G. OTHMAN ◽  
A. KRIEGESKORTE ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Nasal Carriage ◽  
Control Measures ◽  
Coagulase Negative Staphylococci ◽  
Carriage Rate ◽  
Methicillin Resistant ◽  
Infection Control Measures ◽  
Type Iv ◽  
Encoding Genes

SUMMARYThe prevalence of natural carriage and molecular epidemiology of methicillin-resistantStaphylococcus aureus(MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolates in a Jordanian community were investigated. The MRSA nasal carriage rate in 227 healthy volunteers was 7·5% and the majority (81%) of MRSA harboured the resistance element SCCmectype IVe and were of a novelspatype t9519 (76%); other significantspagene types were t223 (14·7%) and t044 (5·9%). All MRSA isolates were susceptible to other classes of antibiotics, and tested positive for at least three virulence factor encoding genes, but only two harboured thepvlgene. MR-CoNS carriage was 54·2% and these isolates were characterized by single, double and untypable SCCmecelements, withStaphylococcus epidermidisSCCmectype IVa predominating. Of eight subjects with nasal co-colonization of MR-CoNS + MRSA, three shared SCCmectype IV in both groups of organisms. This is the first report of methicillin-resistant staphylococci carriage in a Jordanian community and its findings are important for epidemiological study and infection control measures of these organisms.

scholarly journals Characterization of a Novel Arginine Catabolic Mobile Element (ACME) and Staphylococcal Chromosomal CassettemecComposite Island with Significant Homology to Staphylococcus epidermidis ACME Type II in Methicillin-Resistant Staphylococcus aureus Genotype ST22-MRSA-IV

2011 ◽  
Vol 55 (5) ◽  
pp. 1896-1905 ◽  
Author(s):  
Anna C. Shore ◽  
Angela S. Rossney ◽  
Orla M. Brennan ◽  
Peter M. Kinnevey ◽  
Hilary Humphreys ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Mobile Element ◽  
Type I ◽  
Type Ii ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Content Type ◽  
Arginine Catabolic Mobile Element

ABSTRACTThe arginine catabolic mobile element (ACME) is prevalent among methicillin-resistantStaphylococcus aureus(MRSA) isolates of sequence type 8 (ST8) and staphylococcal chromosomal cassettemec(SCCmec) type IVa (USA300) (ST8-MRSA-IVa isolates), and evidence suggests that ACME enhances the ability of ST8-MRSA-IVa to grow and survive on its host. ACME has been identified in a small number of isolates belonging to other MRSA clones but is widespread among coagulase-negative staphylococci (CoNS). This study reports the first description of ACME in two distinct strains of the pandemic ST22-MRSA-IV clone. A total of 238 MRSA isolates recovered in Ireland between 1971 and 2008 were investigated for ACME using a DNA microarray. Twenty-three isolates (9.7%) were ACME positive, and all were either MRSA genotype ST8-MRSA-IVa (7/23, 30%) or MRSA genotype ST22-MRSA-IV (16/23, 70%). Whole-genome sequencing and comprehensive molecular characterization revealed the presence of a novel 46-kb ACME and staphylococcal chromosomal cassettemec(SCCmec) composite island (ACME/SCCmec-CI) in ST22-MRSA-IVh isolates (n= 15). This ACME/SCCmec-CI consists of a 12-kb DNA region previously identified in ACME type II inS. epidermidisATCC 12228, a truncated copy of the J1 region of SCCmectype I, and a complete SCCmectype IVh element. The composite island has a novel genetic organization, with ACME located withinorfXand SCCmeclocated downstream of ACME. One PVL locus-positive ST22-MRSA-IVa isolate carried ACME located downstream of SCCmectype IVa, as previously described in ST8-MRSA-IVa. These results suggest that ACME has been acquired by ST22-MRSA-IV on two independent occasions. At least one of these instances may have involved horizontal transfer and recombination events between MRSA and CoNS. The presence of ACME may enhance dissemination of ST22-MRSA-IV, an already successful MRSA clone.

Molecular Characterization of Methicillin-Resistant Coagulase-Negative Staphylococci from a Neonatal Intensive Care Unit

2002 ◽  
Vol 23 (8) ◽  
pp. 447-451 ◽  
Author(s):  
Isabel Bogado ◽  
Adriana Limansky ◽  
Emma Sutich ◽  
Patricia Marchiaro ◽  
Marta Marzi ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Molecular Characterization ◽  
Neonatal Intensive Care ◽  
Genomic Diversity ◽  
Phenotypic Characterization ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant

Objective:To evaluate clonal dissemination of methicillin-resistant coagulase-negative staphylococci (CNS).Setting:Neonatal intensive care unit of a 180-bed, university-affiliated general hospital.Patients:Neonates admitted to the neonatal intensive care unit between March 1999 and October 2000, from whom CNS were isolated as a unique pathogen. Patients from other wards from whom epidemiologically unrelated staphylococci strains were obtained served as control-patients.Methods:Conventional methods were used for phenotypic characterization of CNS. Methicillin resistance was determined bymecA polymerase chain reaction (PCR) amplification. Genotypic characterization was done by random amplification of DNA with degenerated primers (RAPD) and repetitive element sequence-based PCR (rep-PCR).Results:Forty methicillin-resistant CNS isolates obtained from neonates were characterized asStaphylococcus epidermidis(33),S. hominis(5),S. warneri(1), andS. auricularis(1). Both RAPD and rep-PCR indicated the presence of 4 different clones among the 33S. epidermidisisolates. In turn, the 4 randomly selected, epidemiologically unrelated methicillin-resistant CNS strains obtained from control-patients showed 3 new profiles by RAPD and 2 by rep-PCR, which differed from the corresponding patterns mentioned earlier. Persistence ofS. hominisin a neonate could be assessed by both genotypic techniques.Conclusions:The molecular characterization of the methicillin-resistant CNS studied indicated dissemination of one particular methicillin-resistant CNS clone among the neonates in the ward studied. Although RAPD showed a superior power to discriminate among methicillin-resistant CNS isolates, both RAPD and rep-PCR detected intraspecific and interspecific genomic diversity.

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