High Occurrence of Neospora caninum Antibodies in Buffaloes from the Central, Thailand

Neospora caninum é um protozoário cujos hospedeiros definitivos são canídeos domésticos e silvestres, e hospedeiros intermediários são os herbívoros. Objetivou-se determinar a prevalência de anticorpos IgG anti-N. caninum em cães de zona rural do município de Sousa, Paraíba. Foram analisadas amostras de 98 cães de zona rural, através da Reação de Imuno fluorescência Indireta no ponto de corte 1:50. A frequência de soro positividade foi de 9,18% (9/98). A titulação de anticorpos variou de 50 a 200, predominando o título 1:50. Em 66,6% (4/6) das propriedades avaliadas havia pelo menos um cão soropositivo. O contato com bovinos foi considerado fator de risco (Odds ratio=15,25) para a infecção, demonstrando maior risco de contato com tecidos contaminados. Conclui-se que os cães de zona rural do município de Sousa estão expostos ao N. caninum.

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Seroprevalence of Antibodies to Neospora caninum in Urban and Rural Dogs in north-west Italy

Zoonoses and Public Health ◽

10.1111/j.1863-2378.2007.01033.x ◽

2007 ◽

Vol 54 (3-4) ◽

pp. 135-139 ◽

Cited By ~ 13

Author(s):

E. Ferroglio ◽

M. Pasino ◽

F. Ronco ◽

A. Benà ◽

A. Trisciuoglio

Keyword(s):

Neospora Caninum ◽

North West

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Spatial distance between sites of sampling associated with genetic variation among Neospora caninum in aborted bovine foetuses from northern Italy

Parasites & Vectors ◽

10.1186/s13071-020-04557-6 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Luca Villa ◽

Pavlo Maksimov ◽

Christine Luttermann ◽

Mareen Tuschy ◽

Alessia L. Gazzonis ◽

...

Keyword(s):

Genetic Distance ◽

Neospora Caninum ◽

Geographic Distance ◽

Rare Event ◽

Northern Italy ◽

Spatial Distance ◽

Italian Regions ◽

Bovine Abortion

Abstract Background Neospora caninum, a coccidian protozoan, represents an important cause of bovine abortion. Available N. caninum strains show considerable variation in vitro and in vivo, including different virulence in cattle. To which extent sexual recombination, which is possible in the intestines of domestic dogs and closely related carnivores as definitive hosts, contributes to this variation is not clear yet. Methods Aborted bovine foetuses were collected between 2015 and early 2019 from Italian Holstein Friesian dairy herds suffering from reproductive problems. A total of 198 samples were collected from 165 intensive farms located in Lombardy, northern Italy. N. caninum samples were subjected to multilocus-microsatellite genotyping using ten previously established microsatellite markers. In addition to our own data, those from a recent study providing data on five markers from other northern Italian regions were included and analysed. Results Of the 55 samples finally subjected to genotyping, 35 were typed at all or 9 out of 10 loci and their individual multilocus-microsatellite genotype (MLMG) determined. Linear regression revealed a statistically significant association between the spatial distance of the sampling sites with the genetic distance of N. caninum MLMGs (P < 0.001). Including data from this and a previous North Italian study into eBURST analysis revealed that several of N. caninum MLMGs from northern Italy separate into four groups; most of the samples from Lombardy clustered in one of these groups. Principle component analysis revealed similar clusters and confirmed MLMG groups identified by eBURST. Variations observed between MLMGs were not equally distributed over all loci, but predominantly observed in MS7, MS6A, or MS10. Conclusions Our findings confirm the concept of local N. caninum subpopulations. The geographic distance of sampling was associated with the genetic distance as determined by microsatellite typing. Results suggest that multi-parental recombination in N. caninum is a rare event, but does not exclude uniparental mating. More comprehensive studies on microsatellites in N. caninum and related species like Toxoplasma gondii should be undertaken, not only to improve genotyping capabilities, but also to understand possible functions of these regions in the genomes of these parasites.

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Salmonella in Captive Reptiles and Their Environment—Can We Tame the Dragon?

Microorganisms ◽

10.3390/microorganisms9051012 ◽

2021 ◽

Vol 9 (5) ◽

pp. 1012

Author(s):

Magdalena Zając ◽

Magdalena Skarżyńska ◽

Anna Lalak ◽

Renata Kwit ◽

Aleksandra Śmiałowska-Węglińska ◽

...

Keyword(s):

Public Health ◽

Antimicrobial Resistance ◽

Detection Rate ◽

Large Scale ◽

Isolation And Identification ◽

Epidemiological Analysis ◽

High Occurrence ◽

Salmonella Serovars ◽

Floor Surfaces

Reptiles are considered a reservoir of a variety of Salmonella (S.) serovars. Nevertheless, due to a lack of large-scale research, the importance of Reptilia as a Salmonella vector still remains not completely recognized. A total of 731 samples collected from reptiles and their environment were tested. The aim of the study was to assess the prevalence of Salmonella in exotic reptiles kept in Poland and to confirm Salmonella contamination of the environment after reptile exhibitions. The study included Salmonella isolation and identification, followed by epidemiological analysis of the antimicrobial resistance of the isolates. Implementation of a pathway additional to the standard Salmonella isolation protocol led to a 21% increase in the Salmonella serovars detection rate. The study showed a high occurrence of Salmonella, being the highest at 92.2% in snakes, followed by lizards (83.7%) and turtles (60.0%). The pathogen was also found in 81.2% of swabs taken from table and floor surfaces after reptile exhibitions and in two out of three egg samples. A total of 918 Salmonella strains belonging to 207 serovars and serological variants were obtained. We have noted the serovars considered important with respect to public health, i.e., S. Enteritidis, S. Typhimurium, and S. Kentucky. The study proves that exotic reptiles in Poland are a relevant reservoir of Salmonella.

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Neospora caninum and/or Toxoplasma gondii Seroprevalence: Vaccination against PCV2 and Muscle Enzyme Activity in Seropositive and Seronegative Pigs

Microorganisms ◽

10.3390/microorganisms9051097 ◽

2021 ◽

Vol 9 (5) ◽

pp. 1097

Author(s):

Labrini V. Athanasiou ◽

Vasileios G. Papatsiros ◽

Victoria M. Spanou ◽

Eleni G. Katsogiannou ◽

Anna Dedousi

Keyword(s):

Enzyme Activity ◽

Toxoplasma Gondii ◽

Neospora Caninum ◽

Human Infection ◽

Porcine Circovirus ◽

Muscle Enzyme ◽

Blood Samples ◽

Zoonotic Pathogen ◽

Muscle Enzyme Activity ◽

Immunofluorescence Antibody

Neospora caninum and Toxoplasma gondii affect both humans and animals worldwide. To investigate their seroprevalence and differences in seropositivity between pigs vaccinated and unvaccinated against porcine circovirus 2 (PCV2), as well as differences in muscle enzyme activity between seropositive and seronegative pigs, blood samples were collected from 380 sows. Antibodies against T. gondii and N. caninum were detected by an indirect immunofluorescence antibody (IFA) assay, while the activities of creatine kinase (CK) and aspartate aminotransferase (AST) were biochemically assessed. Out of the 364 sows finally included in the study, 4.4%, 3.5%, and 0.5% were seropositive to T. gondii, N. caninum, or both. A significantly higher percentage of seropositivity against T. gondii and/or N. caninum in PCV2 unvaccinated pigs compared with vaccinated pigs was observed. Increased serum activities of CK and AST were detected in 71.43% and 100% of only against T. gondii (T+) and 63.64% and 90.91% of only against N. caninum (N+) seropositive sows, respectively, and were significantly higher compared to seronegative animals. T. gondii and N. caninum seropositivity, especially in presumed immunocompromised pigs, and the evidence of muscle damage highlight their importance as a zoonotic pathogen and animal model of human infection, respectively.

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